Volume 11, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Current procedures for cultivation of with provide greater production of amebase than methods previously reported. The newer cultures lend themselves conveniently to centrifugal concentration under reduced oxygen tension. Because oxygenation is thought to be detrimental, this may enhance the vigor of amebae in inocula prepared for investigations. Despite the improved cultivation and harvesting procedures, however, showed decreased virulence for guinea pigs after prolonged association with in A-T medium .

Information is presented on the growth rates of amebae in A-T cultures from measured inocula at various intervals of incubation. Considerable growth of occurs in A-T medium if amebae are not present, but the number of trypanosomes decreases in the presence of vigorous amebic growth. did not grow when species of were substituted for in the A-T medium complex.


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