1921
Volume 9, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Summary

In attempts to develop a specifically reactive complement fixing antigen, desiccated rendered essentially lipid-free by extraction with anhydrous ether were extracted in buffered saline solution and the saline extract treated with chloroform and n-amyl alcohol under conditions considered to minimize deterioration. This yielded protein and carbohydrate antigens separable, respectively, in the gel and aqueous phase of extracts thus treated. Each showed a high degree of specific reactivity, as demonstrated in complement fixation tests of sera representing the homologous and several heterologous infections. The findings indicated that the protein fraction was the antigen of choice for diagnostic complement fixation tests.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1960.9.512
1960-09-01
2017-09-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1960.9.512
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error