Volume 9, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Hemagglutination (HA) and bentonite flocculation (BF) tests with 7 crude and antigens were performed. Antisera prepared in rabbits against these antigens, antisera from patients with proven infections of and serum from patients believed to be infected with were titrated. In both the HA and BF tests absolute specificity was not observed with hydatid fluid, scolices, cyst and membrane antigens of the two cestode species. hydatid fluid proved to be the antigen of choice in both the HA and BF tests of sera from humans with hydatid infections. Diagnostic tests with antisera were not as satisfactory, suggesting that tests with crude antigens will not detect human chronic infections with this species of parasite.

By precipitation with TCA, 7 fractions were obtained from hydatid fluid, and 11 from cysts. A number of these fractions were non-reactive in both the HA and BF tests. Fractionated antigens were more sensitive than whole antigens when used with antisera prepared in rabbits. For diagnostic serology, the purified antigens were in some instances more active in the HA test but less reactive in the BF test. For the diagnosis of hydatid infection, the whole hydatid fluid of porcine origin was as reactive as the antigen fractions. For the diagnosis of hydatid infections, the fractionated antigens prepared from whole cysts were superior.


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