1921
Volume 7, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Summary

The studies reported here show that the Turlock virus can be isolated in a culture system which utilizes trypsinized chick-embryo cells as the substrate for viral growth. Viral strains isolated by this technique can be rapidly identified in a colorimetric neutralization test based on the use of chick-embryo cells, a single dilution of the viral isolate and a single dilution of a pretested standard immune serum.

A series of 222 mosquito-pool suspensions was tested in parallel in mice and embryonated eggs and in chick-embryo-cell cultures; 12 strains of Turlock virus were recovered. The isolation results suggest that the tissue-culture method used was comparable in sensitivity to the mouse-embryonated-egg host system.

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/content/journals/10.4269/ajtmh.1958.7.536
1958-09-01
2017-09-20
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