Volume 7, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Comparisons of different techniques for the isolation of from naturally infected hosts were made with the following findings:

  • 1.  The use of multiple-animal passages did not result in the recovery of a significantly larger number of infections.
  • 2.  The use of cortisone in the dosages employed in the present work did not result in increased recovery rates.
  • 3.  Intraperitoneal inoculation was found to be much more efficient than intracerebral inoculation, possibly due to the much larger inocula which could be employed.
  • 4.  Intraperitoneal inoculation followed by sacrifice of inoculated mice at 1 and 2 weeks was about as efficient as intraperitoneal inoculation of mice followed by serological testing and examination at 6 to 8 weeks.
  • 5.  Infection was much less efficient than intraperitoneal inoculation, but was successful on a number of occasions.
  • 6.  Rabbits inoculated intraperitoneally became infected no more consistently than mice in spite of much larger inocula, thus indicating no advantage in the use of this animal.
  • 7.  An efficient technique for routine use was devised.


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