Volume 6, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Certain experiments have recently been designed in which it was desirable to transfer specific strains of from the Shaffer-Frye (S-F) medium to a diphasic medium with the mixed bacterial flora of another strain of . In order to accomplish this it was necessary to separate the particular flora to be used from its accompanying strain of amebas. It was routine practice to take a loopful (4 mm.) of inoculum from the top of the fluid column of the culture of in diphasic medium and transfer it to a similar medium. Since the multiplication of the amebas in such a culture occurs in the bottom of the fluid phase, it seemed unlikely that any amebas would be carried over with a loop transfer taken from the top. It was therefore surprising to find that in a high per cent of such transfers was present when the cultures were examined after 24 to 48 hours incubation at 37° C.


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