Volume 2, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


While experimental culture of has been attempted for many years, few persons have succeeded in obtaining a pure culture, i.e. a culture without bacteria. Trussel (1940) obtained a bacteria-free culture of , and later Adler and Pulvertaft (1944) and Johnson, Trussel, and Jahn (1945) succeeded in obtaining a pure culture by utilizing penicillin. Subsequently Quisno and Foter (1945) and others reported experiments in which penicillin and streptomycin were utilized. In our country, Asami (1952), by using an original culture method and adding penicillin, succeeded in making a pure culture of .

In view of the fact that a pure culture is necessary in order to know the various biological properties of , to ascertain its pathogenicity to the human body and for the study of medicines for its treatment, we made experiments for this purpose and succeeded easily in culturing free from bacteria as shown below.


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