Volume 2, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Determinations on the numbers of amebae obtainable from cultures of with 17 individual species of bacterial symbionts in whole-egg medium showed differences ranging from 20,000 to 450,000 amebae per culture.

-organism could not be propagated through indefinite serial transfer in egg white medium even with enrichments of cholesterol and B vitamins. With enrichment of cholesterol the ameba was propagated in egg white medium with and , respectively, through indefinite serial transfer.

Evidence was obtained indicating that produces a gelatinase and/or a protease that liberates starch grains from particles of ground rice, and that rice proteolysis may be adaptive.

Using the Eldredge-tube method of measuring carbon dioxide, more of the gas was demonstrable from cultures of with two bacterial symbionts than from the symbiont alone except when glucose-enriched medium was used. The increase was attributable to substrate furnished through the metabolism of the amebae. No differences in production of carbon dioxide were demonstrable from cultures of , and of without amebae. This result was attributed to utilization of rice flour by .


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