Volume 1, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



  • 1.  Trophozoites from various cultures of strain 200 were evaluated as to their infectivity for rabbits.
  • 2.  When cultivated with a mixed bacterial flora, strain 200 produced a fatal ulcerative amebiasis in about 85 per cent of the inoculated animals as compared to 35 per cent when cultivated with a single bacterial associate, organism .
  • 3.  Trophozoites from bacteria-free cultures, with as an associate, produced but one infection when inoculated into 24 animals.
  • 4.  The infectivity of trophozoites of grown with was restored after the amebae were re-established in LER culture with bacteria.
  • 5.  observations demonstrated that amebae grown in the absence of bacteria (amebae- cultures) became readapted to an environment with bacteria only with difficulty. Hence, this is offered as a possible explanation for the low infectivity of these amebae when inoculated intracecally into rabbits, although the possible influence of encystation on infectivity must be considered.


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