Volume s1-31, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



  • 1.  heat-treated at 45°C., 48°C., and 50°C. for 10 minutes survived when transplanted onto unheated or trypanosomes heated at 45°C. and 48°C. for 10 minutes, but failed to survive when associated with trypanosomes heat-treated at 50°C.
  • 2.  Subcultures of after heating at 48°C. and 50°C. failed to produce growth.
  • 3.  Oxygen uptake studies indicated that there is a pronounced respiration with 1 ml. of with a count of 100,000,000 per ml. heat-treated at 48°C. for 10 minutes, but no respiration occurs with heat-treated at 50°C. for 10 minutes.
  • 4.  The efficacy of associates in the cultivation of was demonstrated after heat-treatment that rendered the trypanosomes non-viable but did not extend to temperatures that inhibited their respiration.


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