Volume s1-31, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



  • 1.  Investigations were made on effects of acetic acid and some other chemical compounds on larvae of the dog and cat hookworm, , in fecal-soil cultures.
  • 2.  The sources of hookworm eggs were worms in two young cats which were inoculated subcutaneously with larvae from a dog infection. Larvae were recovered from fecal-soil cultures with the aid of a Baermann apparatus.
  • 3.  Results of effects of chemicals on hookworm larvae introduced into soil cultures demonstrated that all the compounds tested, acetic acid in particular, acted to some extent upon the larvae.
  • 4.  Applications of five or 10 per cent concentrations of acetic acid to cat fecal-soil cultures for 24 hours after the larvae appeared at the surface of the culture killed over 98 per cent of the larvae in the cultures as determined by the number of surviving larvae in the control cultures.
  • 5.  Application of 10 per cent acetic acid to newly-made cat fecal-soil cultures inhibited hatching of hookworm eggs for two days, and limited hookworm survival to 2.1 larvae per gram of fecal material during the first five days of treatment.
  • 6.  The following 10 chemical compounds tested for the first time on hookworm or other strongyle larvae exhibited some lethal action: trichloroacetic acid, lactic acid, tartaric acid, citric acid, sodium acetate, calcium lactate, lead acetate, ferric acetate, manganese chloride and amyl acetate.
  • 7.  The order of highest effectiveness of eight of the chemicals tested against hookworm larvae was acetic acid, trichloroacetic acid, oxalic acid, citric acid, lactic acid, tartaric acid, ammonium sulfate and sodium hydrogen phosphate.


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