Volume s1-30, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



  • 1.  Bacteria-free cultures of with active have been maintained through 50 serial transfers and are in use for the production of antigens and for the inoculation of experimental animals with the ameba.
  • 2.  Similar cultures, with heat-treated at 48°C. for 10 minutes, have been maintained through 25 serial transfers.
  • 3.  Some growth of amebas was demonstrated in microtubes with heat-treated at 48°C. for 10 minutes; no growth of amebas could be demonstrated with treated at 52°C.
  • 4.  Rich suspensions of were required for good growth of in microcultures.
  • 5.  failed to grow with rich suspensions of from a dialysate medium in routine use for the cultivation of the trypanosome.
  • 6.  A new experimental method of approach has been provided for problems of amebiasis.


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