Volume s1-24, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



In Florence flask cultures of the L.E.S. medium the average yield of , based on 20 experiments, was 1,530,000 per flask but was scanty in media made either from egg white or egg yolk.

The addition to egg white medium of cholesterol and 8 vitamins of the B complex group stimulated growth of to a point well over 1,000,000 amoebae per flask but was without effect on yolk medium.

Cholesterol alone or the vitamins alone in egg white medium had no effect on growth of .

The counts of amoebae obtained from medium enriched with the amino acids of casein hydrolysate and with the 4 purine bases were slightly higher than those from an unenriched medium.

Two commercial products of dehydrated egg albumin did not support growth of .

Ovalbumin, ovomucin and ovomucoid when added in combination to yolk medium showed some stimulating effect on the growth of .

In one instance an infusate of coagulated egg white plus the vitamins and cholesterol produced a fair crop of amoebae.


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  • Received : 08 Dec 1943
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