1921
Volume s1-15, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

In the for July, 1927, Colonel Charles F. Craig, M. C., U. S. A., described a complement-fixation test for detecting the presence of infection with . Ever since that time efforts have been made to perfect a pure antigen, free from bacterial content. The antigen prepared by the following method is practically bacteria-free and seems to be of greater value as a diagnostic aid in amebiasis than those formerly used.

PREPARATION OF ANTIGEN

  • 1.  . Take twelve to twenty cultures (prepared as recommended in the preceding paper), containing on an average 1,800,000 cysts per culture, and allow them to stand in a vertical position in an ice-box for twenty-four hours. All of the cysts will now be in the bottom of the tubes. Carefully draw off and discard all but 1 cc. of the supernatant fluid by means of a suction pump, large dropper or pipette.

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/content/journals/10.4269/ajtmh.1935.s1-15.685
1935-11-01
2017-11-22
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  • Received : 26 Jul 1935

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