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Many workers in the field of protozoology have observed the occasional occurrence of encystment in cultures of Endamoeba histolytica. This is particularly true when the culture medium used contains starch granules. However, as far as the author is aware, no one has been able to so influence cultures of E. histolytica as to cause such encystment consistently. During the past six months encystment of cultures of E. histolytica has been produced daily by use of the following technique: