Volume s1-15, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The trophozoites of and and of have been observed in aqueous smears and the essential findings recorded. Material for a similar study of the trophozoites of has not been available. The observations indicate that certain features in the morphology of the amoebae can be demonstrated in the aqueous medium, such as (1) the distensibility of the ectoplasm of the , (2) the relative fragility of the ectoplasm of the and , (3) the resiliency of the ectoplasm of the , (4) the remarkable strength of the nuclear membrane of the and .

Some of these characteristics together with the visibility of the nuclei help to differentiate the species of amoebae in the human stool.

It also has been recorded that the annoyance of can be eliminated by the use of the aqueous smear.

I have been unable to find any reference in the literature to the use of aqueous smears in the study of the amoebae of man. However, it does not seem possible that such a simple procedure has not been used by some workers.

It is my pleasure to acknowledge the generous and valuable advice of Dr. C. M. Johnson, protozoölogist at the Gorgas Memorial Laboratory and the efficient assistance of J. F. Buckner and H. A. Down of the Hospital Corps, U. S. Navy.


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