Volume s1-6, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



  • 1.  A medium consisting of Locke solution containing one part to seven of inactivated human, horse, or rabbit blood serum has been found very efficient in the cultivation of . The best results were obtained when inactivated human blood serum was used.
  • 2.  The excellent growth of obtained in this medium demonstrates that coagulated egg or blood agar slants used as a sub-stratum, are not essential for the growth and development of this species, and that neither egg or blood are essential as a part of the food supply of this species.
  • 3.  In this medium has remained alive and motile for a period of eleven days and successful transfers have been obtained in the medium after a period of eight days. The best results in transferring cultures are obtained by making transfers every twenty-four or forty-eight hours.
  • 4.  Transfers of , in this medium, made at intervals of twenty-four and forty-eight hours, have been continued for over three months in this laboratory and the amoebae have not changed in morphology or in their capability for reproduction during this time.


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