1921
Volume 102, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Malaria antigen detection through rapid diagnostic tests (RDTs) is widely used to diagnose malaria and estimate prevalence. To support more sensitive next-generation RDT development and screen asymptomatic malaria, we developed and evaluated the Q-Plex Human Malaria Array (Quansys Biosciences, Logan, UT), which quantifies the antigens commonly used in RDTs—–specific histidine-rich protein 2 (HRP2), specific lactate dehydrogenase ( LDH), specific LDH ( LDH), and Pan malaria lactate dehydrogenase (Pan LDH), and human C-reactive protein (CRP), a biomarker of severity in malaria. At threshold levels yielding 99.5% or more diagnostic specificity, diagnostic sensitivities against polymerase chain reaction–confirmed malaria for HRP2, LDH, LDH, and Pan LDH were 92.7%, 71.5%, 46.1%, and 83.8%, respectively. culture strains and samples from Peru indicated that HRP2 and  LDH combined improves detection of parasites with and deletions. This array can be used for antigen-based malaria screening and detecting deletion mutants of .

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Supplemental file

  • Received : 14 Oct 2019
  • Accepted : 13 Jan 2020
  • Published online : 16 Mar 2020
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