Volume 102, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Host seeking is an essential process in mosquito reproduction. Field releases of modified mosquitoes for population replacement rely on successful host seeking by female mosquitoes, but host-seeking ability is rarely tested in a realistic context. We tested the host-seeking ability of female mosquitoes using a semi-field system. Females with different infection types (Mel-, AlbB-infected, and uninfected) or from different origins (laboratory and field) were released at one end of a semi-field cage and recaptured as they landed on human experimenters 15 m away. Mosquitoes from each population were then identified with molecular tools or through minimal dusting with fluorescent powder. -infected and uninfected populations had similar average durations to landing and overall recapture proportions, as did laboratory and field-sourced These results indicate that the host-seeking ability of mosquitoes is not negatively affected by infection or long-term laboratory maintenance. This method provides an approach to study the host-seeking ability of mosquitoes in a realistic setting, which will be useful when evaluating strains of mosquitoes that are planned for releases into the field to suppress arbovirus transmission.


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Supplemental tables and figures

  • Received : 08 Jul 2019
  • Accepted : 04 Oct 2019
  • Published online : 25 Nov 2019
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