Volume 101, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A substantial decline of malaria transmission intensity has been observed in sub-Saharan Africa over the past two decades and may affect the diagnostic performance of malaria rapid diagnostic test (mRDT) and microscopy. Diagnostic performance of histidine-rich protein II (HRP-II)/pan-lactate dehydrogenase (pLDH)–based mRDT and microscopy was evaluated against polymerase chain reaction (PCR) for the diagnosis of infection among 316 primary schoolchildren in Kibiti district, in 2016. Polymerase chain reaction detected more cases of infection than mRDT or microscopy. Using PCR as reference, the sensitivity and specificity of mRDT were 75.9% (95% CI = 62.8–86.1) and 96.9% (95% CI = 94.0–98.7), respectively, whereas that of microscopy were 63.8% (95% CI = 50.1–76.0) and 95.7% (95% CI = 92.5–97.9), respectively. Polymerase chain reaction and other molecular methods should be considered for use in schools and other epidemiological surveys as supplement to mRDT or microscopy.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...


Data & Media loading...

  • Received : 23 May 2019
  • Accepted : 11 Jul 2019
  • Published online : 19 Aug 2019

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error