1921
Volume 102, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Proposed interventions for eliminating drug-resistant malaria include the targeting of asymptomatic carriers through screening and treatment. We report on the diagnostic performance of the recently developed ultrasensitive rapid diagnostic test (uRDT) compared with screening with conventional RDTs (cRDT) and polymerase chain reaction (PCR) under field conditions in Cambodia in a total of 2,729 individuals. The positivity by quantitative PCR (qPCR) was 3.8% (26/678) in those screened during active case detection and 0.5% (10/2,051) in the cross-sectional survey. Compared with qPCR, the sensitivity of the uRDTs was 53.8% (95% CI: 33.4–73.4%) when used in active case detection and 60.0% (95% CI: 26.2–87.8%) in the cross-sectional survey. The uRDTs did not show a significant improvement in diagnostic performance over cRDTs when used for active case detection and for a malaria prevalence survey in the context of this low-transmission setting.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.19-0349
2019-12-09
2020-08-13
Loading full text...

Full text loading...

/deliver/fulltext/14761645/102/2/tpmd190349.html?itemId=/content/journals/10.4269/ajtmh.19-0349&mimeType=html&fmt=ahah

References

  1. Sochantha T, Hewitt S, Nguon C, Okell L, Alexander N, Yeung S, Vannara H, Rowland M, Socheat D, 2006. Insecticide-treated bednets for the prevention of Plasmodium falciparum malaria in Cambodia: a cluster-randomized trial. Trop Med Int Health 11: 11661177.
    [Google Scholar]
  2. WHO, 2018. World Malaria Report 2018. Geneva, Switzerland: World Health Organization.
    [Google Scholar]
  3. Ashley EA et al., Tracking Resistance to Artemisinin Collaboration, 2014. Spread of artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med 371: 411423.
    [Google Scholar]
  4. Ministry of Health Kingdom of Cambodia, 2017. Cambodia Malaria Elimination Action Framework (2016–2020) | MESA. Available at: http://mesamalaria.org/resource-hub/cambodia-malaria-elimination-action-framework-2016-2020. Accessed October 1, 2019.
    [Google Scholar]
  5. Bousema T, Okell L, Felger I, Drakeley C, 2014. Asymptomatic malaria infections: detectability, transmissibility and public health relevance. Nat Rev Microbiol 12: 833840.
    [Google Scholar]
  6. Gryseels C et al., 2015. High mobility and low use of malaria preventive measures among the Jarai male youth along the Cambodia-Vietnam border. Am J Trop Med Hyg 93: 810818.
    [Google Scholar]
  7. Guyant P, Canavati SE, Chea N, Ly P, Whittaker MA, Roca-Feltrer A, Yeung S, 2015. Malaria and the mobile and migrant population in Cambodia: a population movement framework to inform strategies for malaria control and elimination. Malar J 14: 252.
    [Google Scholar]
  8. Bannister-Tyrrell M et al., 2019. Forest goers and multidrug-resistant malaria in Cambodia: an ethnographic study. Am J Trop Med Hyg 100: 11701178.
    [Google Scholar]
  9. Das S et al., 2017. Performance of a high-sensitivity rapid diagnostic test for Plasmodium falciparum malaria in asymptomatic individuals from Uganda and Myanmar and naive human challenge infections. Am J Trop Med Hyg 97: 15401550.
    [Google Scholar]
  10. Hoyer S et al., 2012. Focused screening and treatment (FSAT): a PCR-based strategy to detect malaria parasite carriers and contain drug resistant P. falciparum, Pailin, Cambodia. PLoS One 7: e45797.
    [Google Scholar]
  11. Canier L et al., 2015. Malaria PCR detection in Cambodian low-transmission settings: dried blood spots versus venous blood samples. Am J Trop Med Hyg 92: 573577.
    [Google Scholar]
  12. Hofmann NE et al., 2018. Assessment of ultra-sensitive malaria diagnosis versus standard molecular diagnostics for malaria elimination: an in-depth molecular community cross-sectional study. Lancet Infect Dis 10: 11081116.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.19-0349
Loading
/content/journals/10.4269/ajtmh.19-0349
Loading

Data & Media loading...

  • Received : 06 May 2019
  • Accepted : 01 Oct 2019
  • Published online : 09 Dec 2019

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error