1921
Volume 101, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Angiostrongyliasis is a foodborne disease caused by a zoonotic nematode, , which produces eosinophilic meningitis or meningoencephalitis (EOM) in humans. Definitive diagnosis is rarely possible because worms are almost never recovered from patients. Human disease can be diagnosed by clinical symptoms and serological tests. Presently, diagnosis is performed by serological detection of antibodies against specific somatic antigens (molecular mass 29–31 kDa) extracted from female worms. The life cycle of must be maintained in the laboratory to provide a source of this diagnostic antigen. Here, we cloned and expressed recombinant galectin-2 (rAcGal2) corresponding to a 31-kDa antigenic peptide. Recombinant protein was purified and used in immunoblot tests, which showed reactions with human serum panels consisting of six confirmed angiostrongyliasis and 24 clinically diagnosed cases of EOM-associated with angiostrongyliasis, 160 samples from patients with other parasitic infections, and 30 samples from normal healthy subjects. Accuracy, sensitivity, specificity, and positive and negative predictive values were 95.0%, 93.3%, 95.3%, 75.7%, and 98.9%, respectively. The test was nonreactive with sera of human gnathostomiasis and cysticercosis, two diseases that could present similar neurological symptoms. Recombinant AcGal2 has potential as a diagnostic antigen and could replace native parasite antigens in further development of an angiostrongyliasis serodiagnostic test kit.

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References

  1. Cross JH, 1978. Clinical manifestations and laboratory diagnosis of eosinophilic meningitis syndrome associated with angiostrongyliasis. Southeast Asian J Trop Med Public Health 9: 161170.
    [Google Scholar]
  2. Pien FD, Pien BC, 1999. Angiostrongylus cantonensis eosinophilic meningitis. Int J Infect Dis 3: 161163.
    [Google Scholar]
  3. Tsai HC et al., 2001. Eosinophilic meningitis caused by Angiostrongylus cantonensis: report of 17 cases. Am J Med 111: 109114.
    [Google Scholar]
  4. Wang QP, Lai DH, Zhu XQ, Chen XG, Lun ZR, 2008. Human angiostrongyliasis. Lancet Infect Dis 8: 621630.
    [Google Scholar]
  5. Eamsobhana P, Lim PE, Yong HS, 2013. Genetic diversity of the rat lungworm, Angiostrongylus cantonensis, the major cause of eosinophilic meningitis. Hawaii J Med Public Health 72: 1517.
    [Google Scholar]
  6. Thiengo SC, Simoes RO, Fernandez MA, Maldonado A, 2013. Angiostrongylus cantonensis and rat lungworm disease in Brazil. Hawaii J Med Public Health 72: 1822.
    [Google Scholar]
  7. Tsai HC, Chen YS, Yen CM, 2013. Human parasitic meningitis caused by Angiostrongylus cantonensis infection in Taiwan. Hawaii J Med Public Health 72: 2627.
    [Google Scholar]
  8. Wang QP, Chen XG, Lun ZR, 2007. Invasive fresh water snail, China. Emerg Infect Dis 13: 11191120.
    [Google Scholar]
  9. Tesana S, Srisawangwong T, Sithithaworn P, Laha T, Andrews R, 2009. Prevalence and intensity of infection with third stage larvae of Angiostrongylus cantonensis in mollusks from Northeast Thailand. Am J Trop Med Hyg 80: 983987.
    [Google Scholar]
  10. Panackel C, Cherian G, Vijayakumar K, Sharma RN, 2006. Eosinophilic meningitis due to Angiostrongylus cantonensis. Indian J Med Microbiol 24: 220221.
    [Google Scholar]
  11. Lai CH, Yen CM, Chin C, Chung HC, Kuo HC, Lin HH, 2007. Eosinophilic meningitis caused by Angiostrongylus cantonensis after ingestion of raw frogs. Am J Trop Med Hyg 76: 399402.
    [Google Scholar]
  12. Slom TJ et al., 2002. An outbreak of eosinophilic meningitis caused by Angiostrongylus cantonensis in travelers returning from the Caribbean. N Engl J Med 346: 668675.
    [Google Scholar]
  13. Tsai HC, Lee SS, Huang CK, Yen CM, Chen ER, Liu YC, 2004. Outbreak of eosinophilic meningitis associated with drinking raw vegetable juice in southern Taiwan. Am J Trop Med Hyg 71: 222226.
    [Google Scholar]
  14. Punyagupta S, 1979. Angiostrongyliasis: clinical features and human pathology. Cross JH, ed. Studies on Angiostrongyliasis in Eastern Asia and Australia. Taipei, Taiwan: U.S. Naval Medical Research Unit No. 2, 138150.
    [Google Scholar]
  15. Cross JH, Chen ER, 2007. Angiostrongyliasis. Murrell KD, Fried B, eds. Food-Borne Parasitic Zoonoses: Fish and Plant Borne Parasites. New York, NY: Springer, 263290.
    [Google Scholar]
  16. Graeff-Teixeira C, da Silva AC, Yoshimura K, 2009. Update on eosinophilic meningoencephalitis and its clinical relevance. Clin Microbiol Rev 22: 322348.
    [Google Scholar]
  17. Nuamtanong S, 1996. The evaluation of the 29 and 31 kDa antigens in female Angiostrongylus cantonensis for serodiagnosis of human angiostrongyliasis. Southeast Asian J Trop Med Public Health 27: 291296.
    [Google Scholar]
  18. Maleewong W, Sombatsawat P, Intapan PM, Wongkham C, Chotmongkol V, 2001. Immunoblot evaluation of the specificity of the 29-kDa antigen from young adult female worms Angiostrongylus cantonensis for immunodiagnosis of human angiostrongyliasis. Asian Pac J Allergy Immunol 19: 267273.
    [Google Scholar]
  19. Intapan PM, Maleewong W, Sawanyawisuth K, Chotmongkol V, 2003. Evaluation of human IgG subclass antibodies in the serodiagnosis of angiostrongyliasis. Parasitol Res 89: 425429.
    [Google Scholar]
  20. Eamsobhana P, Yoolek A, Punthuprapasa P, Suvouttho S, 2004. A dot-blot ELISA comparable to immunoblot for the specific diagnosis of human parastrongyliasis. J Helminthol 78: 287291.
    [Google Scholar]
  21. Eamsobhana P, Prasartvit A, Gan XX, Yong HS, 2015. Evaluation of dot immunogold filtration assay (DIGFA) for rapid serodiagnosis of eosinophilic meningitis due to Angiostrongylus cantonensis (Nematoda: Metastrongyloidea). Trop Biomed 32: 121125.
    [Google Scholar]
  22. Eamsobhana P, Tungtrongchitr A, Wanachiwanawin D, Yong HS, 2018. Immunochromatographic test for rapid serological diagnosis of human angiostrongyliasis. Int J Infect Dis 73: 6971.
    [Google Scholar]
  23. Morassutti AL, Perelygin A, Levert K, Lin SC, Lee YM, da Silva AJ, Wilkins PP, Graeff-Teixeira C, 2013. Expression of recombinant antigenic proteins from Angiostrongylus cantonensis: a brief report. Hawaii J Med Public Health 72: 5862.
    [Google Scholar]
  24. Elkins DB, Haswell-Elkins M, Anderson RM, 1986. The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection. Trans R Soc Trop Med Hyg 80: 774792.
    [Google Scholar]
  25. Chotmongkol V, Sawanyawisuth K, Thavornpitak Y, 2000. Corticosteroid treatment of eosinophilic meningitis. Clin Infect Dis 31: 660662.
    [Google Scholar]
  26. Somboonpatarakun C, Rodpai R, Intapan PM, Sanpool O, Sadaow L, Wongkham C, Insawang T, Boonmars T, Maleewong W, 2018. Immuno-proteomic analysis of Trichinella spiralis, T. pseudospiralis, and T. papuae extracts recognized by human T. spiralis-infected sera. Parasitol Res 117: 201212.
    [Google Scholar]
  27. Kolaskar AS, Tongaonkar PC, 1990. A semi-empirical method for prediction of antigenic determinants on protein antigens. FEBS Lett 276: 172174.
    [Google Scholar]
  28. Galen RS, 1980. Predictive value and efficiency of laboratory testing. Pediatr Clin North Am 27: 861869.
    [Google Scholar]
  29. Vitta A, Yoshino TP, Kalambaheti T, Komalamisra C, Waikagul J, Ruangsittichai J, Dekumyoy P, 2010. Application of recombinant SMR-domain containing protein of Angiostrongylus cantonensis in immunoblot diagnosis of human angiostrongyliasis. Southeast Asian J Trop Med Public Health 41: 785799.
    [Google Scholar]
  30. Li ZY, Lv ZY, Wei J, Liao Q, Zheng HQ, Wu ZD, 2012. Cloning and characterization of a novel gene encoding 16 kDa protein (Ac16) from Angiostrongylus cantonensis. Parasitol Res 110: 21452153.
    [Google Scholar]
  31. Vitta A, Dekumyoy P, Komalamisra C, Kalambaheti T, Yoshino TP, 2016. Cloning and expression of a 16-kDa recombinant protein from Angiostrongylus cantonensis for use in immunoblot diagnosis of human angiostrongyliasis. Parasitol Res 115: 41154122.
    [Google Scholar]
  32. Cognato BB, Handali S, Morassutti AL, da Silva AJ, Graeff-Teixeira C, 2018. Heterologous expression of three antigenic proteins from Angiostrongylus cantonensis: ES-7, Lec-5, and 14-3-3 in mammalian cells. Mol Biochem Parasitol 221: 3235.
    [Google Scholar]
  33. Eamsobhana P, Tungtrongchitr A, Wanachiwanawin D, Yong HS, Mak JW, 1998. Characterization of a 31-kDa specific antigen from Parastrongylus cantonensis (Nematoda: Metastrongylidae). Int Med Res J 2: 912.
    [Google Scholar]
  34. Morassutti AL, Levert K, Perelygin A, da Silva AJ, Wilkins P, Graeff-Teixeira C, 2012. The 31-kDa antigen of Angiostrongylus cantonensis comprises distinct antigenic glycoproteins. Vector Borne Zoonotic Dis 12: 961968.
    [Google Scholar]
  35. Elola MT, Wolfenstein-Todel C, Troncoso MF, Vasta GR, Rabinovich GA, 2007. Galectins: matricellular glycan-binding proteins linking cell adhesion, migration, and survival. Cell Mol Life Sci 64: 16791700.
    [Google Scholar]
  36. Hainz U, Jurgens B, Heitger A, 2007. The role of indoleamine 2, 3-dioxygenase in transplantation. Transpl Int 20: 118127.
    [Google Scholar]
  37. Hewitson JP, Harcus YM, Curwen RS, Dowle AA, Atmadja AK, Ashton PD, Wilson A, Maizels RM, 2008. The secretome of the filarial parasite, Brugia malayi: proteomic profile of adult excretory-secretory products. Mol Biochem Parasitol 160: 821.
    [Google Scholar]
  38. Klion AD, Donelson JE, 1994. OvGa1BP, a filarial antigen with homology to vertebrate galactoside–binding proteins. Mol Biochem Parasitol 65: 305315.
    [Google Scholar]
  39. Morassutti AL, Levert K, Pinto PM, da Silva AJ, Wilkins P, Graeff-Teixeira C, 2012. Characterization of Angiostrongylus cantonensis excretory-secretory proteins as potential diagnostic targets. Exp Parasitol 130: 2631.
    [Google Scholar]
  40. Zhou R, Caspi RR, 2010. Ocular immune privilege. F1000 Biol Rep 2: 3.
    [Google Scholar]
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  • Received : 15 Mar 2019
  • Accepted : 01 Jul 2019
  • Published online : 05 Aug 2019
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