1921
Volume 101, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract.

is a parasite that causes watery diarrhea among both children and adults. However, because many physicians do not routinely ask for diagnostic test, cryptosporidiosis prevalence is likely underestimated. The current study investigated the prevalence of cryptosporidiosis among children admitted to the hospital with gastrointestinal symptoms. Stool sample was collected from each child and subjected to routine microbiological culture presence was tested by three different methods: real-time PCR (RT-PCR), quick antigen, and microscopic examination with acid-fast staining. Each positive specimen was further tested with BioFire FilmArray Multiplex PCR (bioMérieux SA, Marcy-l'Etoile, France) to determine that is the only pathogen in the sample. Demographic and epidemiological data were collected from the patients’ medical records. Of 291 patient stool samples, nine were positive for or The average age of the nine -positive cases was 2.3 years, lower than the average age of the study population (4.2 years). Of the positive cases, 66.7% were Arabs and 33.3% were Jews. The main complaint of children with was diarrhea. Regarding the laboratory methods for identification, of the positive samples, 100% (9/9) were identified by RT-PCR, 88% (8/9) were positive by antigen test, and only 67% (6/9) were positive by microscopic examination with acid-fast staining. Because of the low incidence of among patients in our facility, the prevalence of cryptosporidiosis still cannot be established. Nevertheless, among the other pathogens found in stool samples, is the second leading cause of hospitalization due to gastrointestinal disease in children in our area.

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  • Received : 20 Jan 2019
  • Accepted : 01 Apr 2019
  • Published online : 20 May 2019

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