1921
Volume 101, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The conventional method of detecting in fecal samples has poor diagnostic sensitivity. Detection of -specific antibodies increases the sensitivity; however, most tests are ELISAs that use parasite extract which may cross-react with the sera of other helminth infections. To improve the serological diagnosis of strongyloidiasis, this study aimed at developing a sensitive and specific lateral flow rapid dipstick test. Two recombinant proteins, recombinant NIE (rNIE) and recombinant Ss1a (rSs1a), were used in preparing the dipstick, with gold-conjugated antihuman IgG4 as detector reagent. In parallel, the corresponding ELISA was performed. Both assays demonstrated diagnostic sensitivity of 91.3% (21/23) when tested with serum samples of patients with infection, and 100% specificity with 82 sera of asymptomatic (healthy) and those with other parasitic infections. The ELISA and dipstick test results were positively correlated to each other ( = 0.6114, = 0.0019). The developed lateral flow dipstick test may improve the serodiagnosis of strongyloidiasis and merit further validation studies.

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References

  1. Schar F, Trostdorf U, Giardina F, Khieu V, Muth S, Marti H, Vounatsou P, Odermatt P, 2013. Strongyloides stercoralis: global distribution and risk factors. PLoS Negl Trop Dis 7: e2288.
    [Google Scholar]
  2. Buonfrate D, Mena MA, Angheben A, Requena-Mendez A, Munoz J, Gobbi F, Albonico M, Gotuzzo E, Bisoffi Z, COHEMI Project Study Group, 2015. Prevalence of strongyloidiasis in Latin America: a systematic review of the literature. Epidemiol Infect 143: 452460.
    [Google Scholar]
  3. Beknazarova M, Whiley H, Ross K, 2016. Strongyloidiasis: a disease of socioeconomic disadvantage. Int J Environ Res Public Health 13: E517.
    [Google Scholar]
  4. Atkins NS, Conway DJ, Lindo JF, Bailey JW, Bundy DA, 1999. L3 antigen-specific antibody isotype responses in human strongyloidiasis: correlations with larval output. Parasite Immunol 21: 517526.
    [Google Scholar]
  5. Prendki V, Fenaux P, Durand R, Thellier M, Bouchaud O, 2011. Strongyloidiasis in man 75 years after initial exposure. Emerg Infect Dis 17: 931932.
    [Google Scholar]
  6. Hasegawa H, Hayashida S, Ikeda Y, Sato H, 2009. Hyper-variable regions in 18S rDNA of Strongyloides spp. as markers for species-specific diagnosis. Parasitol Res 104: 869874.
    [Google Scholar]
  7. Moghaddassani H, Mirhendi H, Hosseini M, Rokni M, Mowlavi G, Kia E, 2011. Molecular diagnosis of Strongyloides stercoralis infection by PCR detection of specific DNA in human stool samples. Iran J Parasitol 6: 2330.
    [Google Scholar]
  8. Verweij JJ, Canales M, Polman K, Ziem J, Brienen EA, Polderman AM, van Lieshout L, 2009. Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR. Trans R Soc Trop Med Hyg 103: 342346.
    [Google Scholar]
  9. ten Hove RJ, van Esbroeck M, Vervoort T, van den Ende J, van Lieshout L, Verweij JJ, 2009. Molecular diagnostics of intestinal parasites in returning travellers. Eur J Clin Microbiol Infect Dis 28: 10451053.
    [Google Scholar]
  10. Saugar JM, Merino FJ, Martin-Rabadan P, Fernandez-Soto P, Ortega S, Garate T, Rodriguez E, 2015. Application of real-time PCR for the detection of Strongyloides spp. in clinical samples in a reference center in Spain. Acta Trop 142: 2025.
    [Google Scholar]
  11. Requena-Mendez A, Chiodini P, Bisoffi Z, Buonfrate D, Gotuzzo E, Munoz J, 2013. The laboratory diagnosis and follow up of strongyloidiasis: a systematic review. PLoS Negl Trop Dis 7: e2002.
    [Google Scholar]
  12. Krolewiecki AJ et al., 2010. Improved diagnosis of Strongyloides stercoralis using recombinant antigen-based serologies in a community-wide study in northern Argentina. Clin Vaccine Immunol 17: 16241630.
    [Google Scholar]
  13. Bisoffi Z et al., 2014. Diagnostic accuracy of five serologic tests for Strongyloides stercoralis infection. PLoS Negl Trop Dis 8: e2640.
    [Google Scholar]
  14. Ramanathan R, Burbelo PD, Groot S, Iadarola MJ, Neva FA, Nutman TB, 2008. A luciferase immunoprecipitation systems assay enhances the sensitivity and specificity of diagnosis of Strongyloides stercoralis infection. J Infect Dis 198: 444451.
    [Google Scholar]
  15. Ravi V, Ramachandran S, Thompson RW, Andersen JF, Neva FA, 2002. Characterization of a recombinant immunodiagnostic antigen (NIE) from Strongyloides stercoralis L3-stage larvae. Mol Biochem Parasitol 125: 7381.
    [Google Scholar]
  16. Arifin N, Yunus MH, Nolan TJ, Lok JB, Noordin R, 2018. Identification and preliminary evaluation of a novel recombinant protein for serodiagnosis of strongyloidiasis. Am J Trop Med Hyg 98: 11651170.
    [Google Scholar]
  17. Rascoe LN, Price C, Shin SH, McAuliffe I, Priest JW, Handali S, 2015. Development of Ss-NIE-1 recombinant antigen based assays for immunodiagnosis of strongyloidiasis. PLoS Negl Trop Dis 9: e0003694.
    [Google Scholar]
  18. Pak BJ et al., 2014. Development of a rapid serological assay for the diagnosis of strongyloidiasis using a novel diffraction-based biosensor technology. PLoS Negl Trop Dis 8: e3002.
    [Google Scholar]
  19. Schneider CA, Rasband WS, Eliceiri KW, 2012. NIH Image to ImageJ: 25 years of image analysis. Nat Methods 9: 671675.
    [Google Scholar]
  20. Arifin N, Hanafiah KM, Ahmad H, Noordin R, 2018. Serodiagnosis and early detection of Strongyloides stercoralis infection. J Microbiol Immunol Infect pii: S1684–1182(18)304262. Available at: https://doi.org/10.1016/j.jmii.2018.10.001.
    [Google Scholar]
  21. Atkins NS, Lindo JF, Lee MG, Conway DJ, Bailey JW, Robinson RD, Bundy DA, 1997. Humoral responses in human strongyloidiasis: correlations with infection chronicity. Trans R Soc Trop Med Hyg 91: 609613.
    [Google Scholar]
  22. Subathra M, Senthilkumar TMA, Ramadass P, Ramaswamy V, 2011. Evaluation of the cocktail recombinant antigens, LipL32 and LipL41 for serodiagnosis of canine leptospirosis. World J Microbiol Biotechnol 27: 10771082.
    [Google Scholar]
  23. Yunus MH, Tan Farrizam SN, Abdul Karim IZ, Noordin R, 2018. A lateral flow rapid test for human toxocariasis developed using three Toxocara canis recombinant antigens. Am J Trop Med Hyg 98: 3238.
    [Google Scholar]
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  • Received : 17 Jan 2019
  • Accepted : 19 May 2019
  • Published online : 17 Jun 2019
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