Volume 101, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We compared the diagnostic performance of the standard method (Kato-Katz) with two recently developed methods (Mini-FLOTAC and Flukefinder) for the detection and quantification of eggs in human stool. Uninfected human stool samples were artificially spiked with eggs to reach final concentrations of 14, 28, 41, or 96 eggs per gram of stool (epg). Only Flukefinder showed 100% sensitivity in all but the samples with the lowest concentration of eggs (14 epg), in which it had a sensitivity of 60%. Each of the methods underestimated the true fecal egg counts (FECs), Flukefinder resulting in the most biased egg counts (egg counts 0.18 times lower than the expected FECs). Only the Flukefinder resulted in more precise results (coefficient of variance < 30%) from FECs of 96 epg onward. The outcome of this study indicates that the Flukefinder is a useful alternative diagnostic method for human fascioliasis in stool.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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  1. Cwiklinski K, O’Neill SM, Donnelly S, Dalton JP, , 2016. A prospective view of animal and human fasciolosis. Parasite Immunol 38: 558568. [Google Scholar]
  2. Mas-Coma S, Bargues MD, Valero MA, , 2018. Human fascioliasis infection sources, their diversity, incidence factors, analytical methods and prevention measures. Parasitology 145: 16651699. [Google Scholar]
  3. Mas-Coma S, Bargues MD, Valero MA, , 2005. Fascioliasis and other plant-borne trematode zoonoses. Int J Parasitol 35: 12551278. [Google Scholar]
  4. Adela Valero M, Perez-Crespo I, Khoubbane M, Artigas P, Panova M, , 2012. Fasciola hepatica phenotypic characterization in Andean human endemic areas: valley versus altiplanic patterns analyzed in liver flukes from sheep from Cajamarca and Mantaro, Peru. Infect Genet Evol 12: 403410. [Google Scholar]
  5. Marcos L, Maco V, Samalvides F, Terashima A, Espinoza JR, Gotuzzo E, , 2006. Risk factors for Fasciola hepatica infection in children: a case-control study. Trans R Soc Trop Med Hyg 100: 158166. [Google Scholar]
  6. Marcos LA, Terashima A, Leguia G, Canales M, Espinoza JR, Gotuzzo E, , 2007. Fasciola hepatica infection in Peru: an emergent disease. Rev Gastroenterol Peru 27: 389396. [Google Scholar]
  7. Cabada MM, Lopez M, Cruz M, Delgado JR, Hill V, White AC, Jr., 2016. Treatment failure after multiple courses of triclabendazole among patients with fascioliasis in Cusco, Peru: a case series. PLoS Negl Trop Dis 10: e0004361. [Google Scholar]
  8. Espinoza JR, Terashima A, Herrera-Velit P, Marcos LA, , 2010. Human and animal fascioliasis in Peru: impact in the economy of endemic zones. Rev Peru Med Exp Salud Publica 27: 604612. [Google Scholar]
  9. Marcos L, Romani L, Florencio L, Terashima A, Canales M, Nestares J, Huayanay L, Gotuzzo E, , 2007. Hyperendemic and mesoendemic zones of Fasciola infection surrounding urban Lima: an emerging disease?. Rev Gastroenterol Peru 27: 3136. [Google Scholar]
  10. Marcos Raymundo LA, Maco Flores V, Terashima Iwashita A, Samalvides Cuba F, Gotuzzo Herencia E, , 2002. Clinical characteristics of chronic infection by Fasciola hepatica in children. Rev Gastroenterol Peru 22: 228233. [Google Scholar]
  11. Lopez M, White AC, Jr. Cabada MM, , 2012. Burden of Fasciola hepatica infection among children from Paucartambo in Cusco, Peru. Am J Trop Med Hyg 86: 481485. [Google Scholar]
  12. Cabada MM, Goodrich MR, Graham B, Villanueva-Meyer PG, Lopez M, Arque E, White AC, Jr., 2014. Fascioliasis and eosinophilia in the highlands of Cuzco, Peru and their association with water and socioeconomic factors. Am J Trop Med Hyg 91: 989993. [Google Scholar]
  13. Gonzalez LC, Esteban JG, Bargues MD, Valero MA, Ortiz P, Naquira C, Mas-Coma S, , 2011. Hyperendemic human fascioliasis in Andean valleys: an altitudinal transect analysis in children of Cajamarca province, Peru. Acta Trop 120: 119129. [Google Scholar]
  14. Mas-Coma S, Bargues MD, Valero MA, , 2014. Diagnosis of human fascioliasis by stool and blood techniques: update for the present global scenario. Parasitology 141: 19181946. [Google Scholar]
  15. Cringoli G, Maurelli MP, Levecke B, Bosco A, Vercruysse J, Utzinger J, Rinaldi L, , 2017. The Mini-FLOTAC technique for the diagnosis of helminth and protozoan infections in humans and animals. Nat Protoc 12: 17231732. [Google Scholar]
  16. Rinaldi L, Gonzalez S, Guerrero J, Aguilera LC, Musella V, Genchi C, Cringoli G, , 2012. A one-health integrated approach to control fascioliasis in the Cajamarca valley of Peru. Geospat Health 6: S67S73. [Google Scholar]
  17. Faria RN, Cury MC, Lima WS, , 2008. Concordância entre duas técnicas coproparasitológicas para diagnóstico de Fasciola hepatica em bovinos. Arq Bras Med Vet Zootec 60: 10231025. [Google Scholar]

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  • Received : 14 Dec 2018
  • Accepted : 14 Mar 2019
  • Published online : 06 May 2019

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