1921
Volume 100, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Gamma irradiation using a cobalt-60 source is a commonly used method for the inactivation of infectious specimens to be handled safely in subsequent laboratory procedures. Here, we determined irradiation doses to safely inactivate liquid proteinaceous specimens harboring different emerging/reemerging viral pathogens known to cause neglected tropical and other diseases of regional or global public health importance. By using a representative arenavirus, bunyavirus, coronavirus, filovirus, flavivirus, orthomyxovirus, and paramyxovirus, we found that these enveloped viruses differed in their susceptibility to irradiation treatment with adsorbed doses for inactivation of a target dose of 1 × 10 50% tissue culture infectious dose (TCID)/mL ranging from 1 to 5 MRads. This finding seemed generally inversely correlated with genome size. Our data may help to guide other facilities in testing and verifying safe inactivation procedures.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.18-0937
2019-03-11
2020-09-19
Loading full text...

Full text loading...

/deliver/fulltext/14761645/100/5/tpmd180937.html?itemId=/content/journals/10.4269/ajtmh.18-0937&mimeType=html&fmt=ahah

References

  1. Sullivan R, Fassolitis AC, Larkin EP, Read RB Jr., Peeler JT, 1971. Inactivation of thirty viruses by gamma radiation. Appl Microbiol 22: 6165.
    [Google Scholar]
  2. Elliott LH, McCormick JB, Johnson KM, 1982. Inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation. J Clin Microbiol 16: 704708.
    [Google Scholar]
  3. Mitchell SW, McCormick JB, 1984. Physicochemical inactivation of Lassa, Ebola, and Marburg viruses and effect on clinical laboratory analyses. J Clin Microbiol 20: 486489.
    [Google Scholar]
  4. House C, Mikiciuk PE, Berninger ML, 1990. Laboratory diagnosis of African horse sickness: comparison of serological techniques and evaluation of storage methods of samples for virus isolation. J Vet Diagn Invest 2: 4450.
    [Google Scholar]
  5. Haddock E, Feldmann F, Feldmann H, 2016. Effective chemical inactivation of Ebola virus. Emerg Infect Dis 22: 12921294.
    [Google Scholar]
  6. Hume AJ, Ames J, Rennick LJ, Duprex WP, Marzi A, Tonkiss J, Mühlberger E, 2016. Inactivation of RNA viruses by gamma irradiation: a study on mitigating factors. Viruses 8: E204.
    [Google Scholar]
  7. Haddock E, Feldmann F, 2017. Validating the inactivation effectiveness of chemicals on Ebola virus. Methods Mol Biol 1628: 251257.
    [Google Scholar]
  8. Division of Select Agents and Toxins (DSAT) of the Centers for Disease Control and Prevention, 2018. Available at: https://www.selectagents.gov/irg-inactivation.html.
  9. Animal and Plant Health Inspection Services of the Department of Agriculture, 2018. Available at: https://www.aphis.usda.gov/aphis/ourfocus/animalhealth/animal-and-animal-product-import-information/sa_ag_select_agent.
  10. Ebihara H et al., 2007. In vitro and in vivo characterization of recombinant Ebola viruses expressing enhanced green fluorescent protein. J Infect Dis 196 (Suppl 2): S313S322.
    [Google Scholar]
  11. Bray M, Davis K, Geisbert T, Schmaljohn C, Huggins J, 1998. A mouse model for evaluation of prophylaxis and therapy of Ebola hemorrhagic fever. J Infect Dis 178: 651661.
    [Google Scholar]
  12. Ebihara H, Takada A, Kobasa D, Jones S, Neumann G, Theriault S, Bray M, Feldmann H, Kawaoka Y, 2006. Molecular determinants of Ebola virus virulence in mice. PLoS Pathog 2: e73.
    [Google Scholar]
  13. Lomax ME, Folkes LK, O’Neill P, 2013. Biological consequences of radiation-induced DNA damage: relevance to radiotherapy. Clin Oncol (R Coll Radiol) 25: 578585.
    [Google Scholar]
  14. Ohshima H, Iida Y, Matsuda A, Kuwabara M, 1996. Damage induced by hydroxyl radicals generated in the hydration layer of gamma-irradiated frozen aqueous solution of DNA. J Radiat Res 37: 199207.
    [Google Scholar]
  15. Jordan RT, Kempe LL, 1956. Inactivation of some animal viruses with gamma radiation from cobalt-60. Proc Soc Exp Biol Med 91: 212215.
    [Google Scholar]
  16. Kenny MT, Albright KL, Emery JB, Bittle JL, 1969. Inactivation of rubella virus by gamma radiation. J Virol 4: 807810.
    [Google Scholar]
  17. Niedrig M, Donoso-Mantke O, Schädler R; ENIVD members, 2007. The European Network for Diagnostics of Imported Viral Diseases (ENIVD)—12 years of strengthening the laboratory diagnostic capacity in Europe. Euro Surveill 2007 Apr 19;12: E070419.5.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.18-0937
Loading
/content/journals/10.4269/ajtmh.18-0937
Loading

Data & Media loading...

  • Received : 23 Nov 2018
  • Accepted : 16 Jan 2019
  • Published online : 11 Mar 2019
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error