1921
Volume 100, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Rotavirus has been one of the major etiological agents causing severe diarrhea in infants and young children worldwide. In Thailand, rotavirus contributes to one-third of reported pediatric diarrheal cases. We studied stool samples from 1,709 children with acute gastroenteritis and 1,761 children with no reported gastroenteritis whose age ranged from 3 months to 5 years from four different regions in Thailand between March 2008 and August 2010. The samples were tested for the presence of rotavirus by real-time reverse transcription–polymerase chain reaction (RT-PCR) amplification of gene and enzyme-linked immunosorbent assay. The positive samples were further characterized for their G and P genotypes ( and genes) by conventional RT-PCR. From all four regions, 26.8% of cases and 1.6% of controls were positive for rotavirus, and G1P[8] was the most predominant genotype, followed by G2P[4], G3P[8], and G9P[8]. In addition, the uncommon genotypes including G1P[4], G1P[6], G2P[6], G2P[8], G4P[6], G9P[4], G9P[6], G12P[6], and G12P[8] were also detected at approximately 14% of all samples tested. Interestingly, G5P[19], a recombinant genotype between human and animal strains, and G1P7[5], a reassortant vaccine strain which is closely related to four human-bovine reassortant strains of RotaTeq vaccine, were detected in control samples. Data reported in this study will provide additional information on molecular epidemiology of rotavirus infection in Thailand before the impending national implementation of rotavirus vaccination program.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.18-0763
2019-03-25
2020-09-19
Loading full text...

Full text loading...

/deliver/fulltext/14761645/100/5/tpmd180763.html?itemId=/content/journals/10.4269/ajtmh.18-0763&mimeType=html&fmt=ahah

References

  1. World Health Organization, 2016. Estimated Rotavirus Deaths for Children under 5 Years of Age: 2013, 215 000. Available at: http://www.who.int/immunization/monitoring_surveillance/burden/estimates/rotavirus/en/. Accessed January 26, 2018.
    [Google Scholar]
  2. Tate JE, Burton AH, Boschi-Pinto C, Parashar UD; World Health Organization-Coordinated Global Rotavirus Surveillance Network, 2016. Global, regional, and national estimates of rotavirus mortality in children <5 years of age, 2000–2013. Clin Infect Dis 62 (Suppl 2): S96S105.
    [Google Scholar]
  3. Matthijnssens J et al., 2008. Recommendations for the classification of group A rotaviruses using all 11 genomic RNA segments. Arch Virol 153: 16211629.
    [Google Scholar]
  4. KU LEUVEN, Laboratory of Viral Metagenomics, 2018. Rotavirus Classification Working Group. Available at: http://www.rega.kuleuven.be/cev/viralmetagenomics/virus-classification/rcwg. Accessed July 23, 2018.
    [Google Scholar]
  5. Santos N, Hoshino Y, 2005. Global distribution of rotavirus serotypes/genotypes and its implication for the development and implementation of an effective rotavirus vaccine. Rev Med Virol 15: 2956.
    [Google Scholar]
  6. Iturriza-Gomara M, 2014. EuroRotaNet: Annual Report 2014. Available at: http://www.thl.fi/documents/533963/1527557/EuroRotaNetAnnualReport6th_2014.pdf/7e4386c0-0e09-4e18-8576-95031eaf2680. Accessed September 5, 2018.
    [Google Scholar]
  7. Patton JT, 2012. Rotavirus diversity and evolution in the post-vaccine world. Discov Med 13: 8597.
    [Google Scholar]
  8. Armah GE, Steele AD, Esona MD, Akran VA, Nimzing L, Pennap G, 2010. Diversity of rotavirus strains circulating in West Africa from 1996 to 2000. J Infect Dis 202: S64S71.
    [Google Scholar]
  9. World Health Organization, 2015. Vaccine Preventable Diseases Surveillance: Global Rotavirus Surveillance and Information Bulletin. Data period: January–December 2013. Available at: http://www.who.int/immunization/monitoring_surveillance/resources/WHO_Global_RV_Surv_Bulletin_Jan_2015_Final.pdf?ua=1. Accessed November 21, 2018.
    [Google Scholar]
  10. Dennehy PH, 2012. Rotavirus infection: an update on management and prevention. Adv Pediatr 59: 4774.
    [Google Scholar]
  11. Maneekarn N, Ushijima H, 2000. Epidemiology of rotavirus infection in Thailand. Pediatr Int 42: 415421.
    [Google Scholar]
  12. Jiraphongsa C, Bresee JS, Pongsuwanna Y, Kluabwang P, Poonawagul U, Arporntip P, Kanoksil M, Premsri N, Intusoma U; Rotavirus Surveillance Project Thailand Study Group, 2005. Epidemiology and burden of rotavirus diarrhea in Thailand: results of sentinel surveillance. J Infect Dis 192 (Suppl 1): S87S93.
    [Google Scholar]
  13. Khamrin P et al., 2006. Emergence of human G9 rotavirus with an exceptionally high frequency in children admitted to hospital with diarrhea in Chiang Mai, Thailand. J Med Virol 78: 273280.
    [Google Scholar]
  14. Khamrin P, Peerakome S, Tonusin S, Malasao R, Okitsu S, Mizuguchi M, Ushijima H, Maneekarn N, 2007. Changing pattern of rotavirus G genotype distribution in Chiang Mai, Thailand from 2002 to 2004: decline of G9 and reemergence of G1 and G2. J Med Virol 79: 17751782.
    [Google Scholar]
  15. Khananurak K, Vutithanachot V, Simakachorn N, Theamboonlers A, Chongsrisawat V, Poovorawan Y, 2010. Prevalence and phylogenetic analysis of rotavirus genotypes in Thailand between 2007 and 2009. Infect Genet Evol 10: 537545.
    [Google Scholar]
  16. Theamboonlers A, Bhattarakosol P, Chongsrisawat V, Sungkapalee T, Wutthirattanakowit N, Poovorawan Y, 2008. Molecular characterization of group A human rotaviruses in Bangkok and Buriram, Thailand during 2004–2006 reveals the predominance of G1P[8], G9P[8] and a rare G3P[19] strain. Virus Genes 36: 289298.
    [Google Scholar]
  17. Maiklang O, Vutithanachot V, Vutithanachot C, Hacharoen P, Chieochansin T, Poovorawan Y, 2012. Prevalence of group A genotype human rotavirus among children with dirarrhea in Thailand, 2009–2011. Southeast Asian J Trop Med Public Health 43: 904916.
    [Google Scholar]
  18. Khamrin P, Maneekarn N, Malasao R, Nguyen TA, Ishida S, Okitsu S, Ushijima H, 2010. Genotypic linkages of VP4, VP6, VP7, NSP4, NSP5 genes of rotaviruses circulating among children with acute gastroenteritis in Thailand. Infect Genet Evol 10: 467472.
    [Google Scholar]
  19. Maneekarn N, Khamrin P, 2014. Rotavirus associated gastroenteritis in Thailand. Virus Dis 25: 201207.
    [Google Scholar]
  20. Silapong S, Sakpaisal P, Bodhidatta L, Lertsethtakarn P, Sethabutr O, Vansith K, Meng CY, Swierczewski BE, Mason CJ, 2017. Genotypic distribution of rotavirus in Phnom Penh, Cambodia: an association of G9 with more severe diseases. Am J Trop Med Hyg 96: 947952.
    [Google Scholar]
  21. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S, 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30: 27252729.
    [Google Scholar]
  22. Chan-It W et al., 2011. Detection and genetic characterization of norovirus infections in children with acute gastroenteritis in Japan, 2007–2009. Clin Lab 57: 213220.
    [Google Scholar]
  23. Chaimongkol N, Khamrin P, Malasao R, Thongprachum A, Ushijima H, Maneekarn N, 2012. Genotypic linkages of gene segments of rotaviruses circulating in pediatric patients with acute gastroenteritis in Thailand. Infect Genet Evol 12: 13811391.
    [Google Scholar]
  24. Arista S, Giammanco GM, De Grazia S, Ramirez S, Lo Biundo C, Colomba C, Cascio A, Martella V, 2006. Heterogeneity and temporal dynamics of evolution of G1 human rotaviruses in a settled population. J Virol 80: 1072410733.
    [Google Scholar]
  25. Abdel-Haq N, Amjad M, McGrath E, Chearskul P, Amer A, Salimnia H, Asmar BI, 2011. Emergence of human rotavirus genotype G9 in metropolitan Detroit between 2007 and 2009. J Med Microbiol 60: 761767.
    [Google Scholar]
  26. Oh HK, Hong SH, Ahn BY, Min HK, 2012. Phylogenetic analysis of the rotavirus genotypes originated from children < 5 years of age in 16 cities in South Korea, between 2000 and 2004. Osong Public Health Res Perspect 3: 3642.
    [Google Scholar]
  27. Dong HJ, Qian Y, Huang T, Zhu RN, Zhao LQ, Zhang Y, Li RC, Li YP, 2013. Identification of circulating porcine-human reassortant G4P[6] rotavirus from children with acute diarrhea in China by whole genome analyses. Infect Genet Evol 20: 155162.
    [Google Scholar]
  28. Mullick S, Mukherjee A, Ghosh S, Pazhani GP, Sur D, Manna B, Nataro JP, Levine MM, Ramamurthy T, Chawla-Sarkar M, 2014. Community based case-control study of rotavirus gastroenteritis among young children during 2008--2010 reveals vast genetic diversity and increased prevalence of G9 strains in Kolkata. Plos One 9: e112970.
    [Google Scholar]
  29. Barnes GL, Callaghan SL, Kirkwood CD, Bogdanovic-Sakran N, Johnston LJ, Bishop RF, 2003. Excretion of serotype G1 rotavirus strains by asymptomatic staff: a possible source of nosocomial infection. J Pediatr 142: 722725.
    [Google Scholar]
  30. Phillips G, Lopman B, Rodrigues LC, Tam CC, 2010. Asymptomatic rotavirus infections in England: prevalence, characteristics, and risk factors. Am J Epidemiol 171: 10231030.
    [Google Scholar]
  31. Maneekarn N, Khamrin P, Chan-it W, Peerakome S, Sukchai S, Pringprao K, Ushijima H, 2006. Detection of rare G3P[19] porcine rotavirus strains in Chiang Mai, Thailand, provides evidence for origin of the VP4 genes of Mc323 and Mc345 human rotaviruses. J Clin Microbiol 44: 41134119.
    [Google Scholar]
  32. Chan-It W, Khamrin P, Saekhow P, Pantip C, Thongprachum A, Peerakorne S, Ushijima H, Maneekarn N, 2008. Multiple combinations of P[13]-like genotype with G3, G4, and G5 in porcine rotaviruses. J Clin Microbiol 46: 11691173.
    [Google Scholar]
  33. Kittigul L, Swangsri T, Pombubpa K, Howteerakul N, Diraphat P, Hirunpetcharat C, 2014. Rotavirus infection in children and adults with acute gastroenteritis in Thailand. Southeast Asian J Trop Med Public Health 45: 816824.
    [Google Scholar]
  34. Yodmeeklin A, Khamrin P, Kumthip K, Malasao R, Ukarapol N, Ushijima H, Maneekarn N, 2018. Increasing predominance of G8P[8] species A rotaviruses in children admitted to hospital with acute gastroenteritis in Thailand, 2010–2013. Arch Virol 163: 21652178.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.18-0763
Loading
/content/journals/10.4269/ajtmh.18-0763
Loading

Data & Media loading...

  • Received : 19 Sep 2018
  • Accepted : 17 Jan 2019
  • Published online : 25 Mar 2019
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error