Volume 99, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



is a cosmopolitan cestode infecting dogs, cats, and humans. Praziquantel is a highly effective cestocidal drug and resistance in adult cestodes has not been reported. From 2016 to 2018, a population of dogs with cestode infections that could not be eliminated despite multiple treatments with praziquantel or epsiprantel was identified. Cases of were clinically resistant to praziquantel and could not be resolved despite increasing the dose, frequency, and duration of treatment. Resistant isolates were identified and characterized by sequencing the 28S, 12S, and voltage-gated calcium channel beta subunit genes. Cases were only resolved following treatment with nitroscanate or a compounded pyrantel/praziquantel/oxantel product. Clinicians should be aware of this alarming development as treatment options for cestodes are limited in both human and veterinary medicine.


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  1. Gates MC, Nolan TJ, , 2009. Endoparasite prevalence and recurrence across different age groups of dogs and cats. Vet Parasitol 166: 153158. [Google Scholar]
  2. Pugh RE, , 1987. Effects on the development of Dipylidium caninum and on the host reaction to this parasite in the adult flea (Ctenocephalides felis felis). Parasitol Res 73: 171177. [Google Scholar]
  3. Zimmermann HR, , 1937. Life-history studies on cestodes of the genus dipylidium from the dog. Zeitschrift für Parasitenkunde 9: 717729. [Google Scholar]
  4. Low VL, Prakash BK, Tan TK, Sofian-Azirun M, Anwar FHK, Vinnie-Siow WY, AbuBakar S, , 2017. Pathogens in ectoparasites from free-ranging animals: infection with Rickettsia asembonensis in ticks, and a potentially new species of Dipylidium in fleas and lice. Vet Parasitol 245: 102105. [Google Scholar]
  5. Tüzer E, Bilgin Z, Oter K, Erçin S, Tinar R, , 2010. Efficacy of praziquantel injectable solution against feline and canine tapeworms. Turkiye Parazitol Derg 34: 1720. [Google Scholar]
  6. Manger BR, Brewer MD, , 1989. Epsiprantel, a new tapeworm remedy. Preliminary efficacy studies in dogs and cats. Br Vet J 145: 384388. [Google Scholar]
  7. Knaus M, 2014. Efficacy of a novel topical fipronil, (S)-methoprene, eprinomectin and praziquantel combination against naturally acquired intestinal nematode and cestode infections in cats. Vet Parasitol 202: 1825. [Google Scholar]
  8. Charles SD, 2005. Evaluation of the efficacy of emodepside+praziquantel topical solution against cestode (Dipylidium caninum, Taenia taeniaeformis, and Echinococcus multilocularis) infections in cats. Parasitol Res 97 (Suppl 1): S33S40. [Google Scholar]
  9. Schroeder I, Altreuther G, Schimmel A, Deplazes P, Kok DJ, Schnyder M, Krieger KJ, , 2009. Efficacy of emodepside plus praziquantel tablets (Profender tablets for dogs) against mature and immature cestode infections in dogs. Parasitol Res 105 (Suppl 1): S31S38. [Google Scholar]
  10. Sharp ML, McCurdy HD, , 1985. Anthelmintic efficacy of febantel combined with praziquantel in dogs. J Am Vet Med Assoc 187: 254255. [Google Scholar]
  11. Cabello RR, Ruiz AC, Feregrino RR, Romero LC, Zavala JT, , 2011. Dipylidium caninum infection. BMJ Case Rep doi: 10.1136/bcr.07.2011.4510. [Google Scholar]
  12. Fourie JJ, Crafford D, Horak IG, Stanneck D, , 2013. Prophylactic treatment of flea-infested dogs with an imidacloprid/flumethrin collar (Seresto®, Bayer) to preempt infection with Dipylidium caninum. Parasitol Res 112 (Suppl 1): 3346. [Google Scholar]
  13. Beugnet F, Delport P, Luus H, Crafford D, Fourie J, , 2013. Preventive efficacy of Frontline® Combo and Certifect® against Dipylidium caninum infestation of cats and dogs using a natural flea (Ctenocephalides felis) infestation model. Parasite 20: 7. [Google Scholar]
  14. Beugnet F, Meyer L, Fourie J, Larsen D, , 2017. Preventive efficacy of NexGard Spectra® against Dipylidium caninum infection in dogs using a natural flea (Ctenocephalides felis) infestation model. Parasite 24: 16. [Google Scholar]
  15. Wang W, Wang L, Liang YS, , 2012. Susceptibility or resistance of praziquantel in human schistosomiasis: a review. Parasitol Res 111: 18711877. [Google Scholar]
  16. Schmid K, Rohdich N, Zschiesche E, Kok DJ, Allan MJ, , 2010. Efficacy, safety and palatability of a new broad-spectrum anthelmintic formulation in dogs. Vet Rec 167: 647651. [Google Scholar]
  17. Beugnet F, Labuschagne M, Fourie J, Jacques G, Farkas R, Cozma V, Halos L, Hellmann K, Knaus M, Rehbein S, , 2014. Occurrence of Dipylidium caninum in fleas from client-owned cats and dogs in Europe using a new PCR detection assay. Vet Parasitol 205: 300306. [Google Scholar]
  18. von Nickisch-Rosenegk M, Lucius R, Loos-Frank B, , 1999. Contributions to the phylogeny of the Cyclophyllidea (Cestoda) inferred from mitochondrial 12S rDNA. J Mol Evol 48: 586596. [Google Scholar]
  19. Kumar S, Stecher G, Tamura K, , 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33: 18701874. [Google Scholar]
  20. Darriba D, Taboada GL, Doallo R, Posada D, , 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9: 772. [Google Scholar]
  21. Jeziorski MC, Greenberg RM, , 2006. Voltage-gated calcium channel subunits from platyhelminths: potential role in praziquantel action. Int J Parasitol 36: 625632. [Google Scholar]
  22. Ito A, Budke CM, , 2014. Culinary delights and travel? A review of zoonotic cestodiases and metacestodiases. Travel Med Infect Dis 12: 582591. [Google Scholar]
  23. Reinemeyer CR, Hutchens DE, Eckblad WP, Marchiondo AA, Shugart JI, , 2006. Dose-confirmation studies of the cestocidal activity of pyrantel pamoate paste in horses. Vet Parasitol 138: 234239. [Google Scholar]
  24. Babes RM, Selescu T, Domocos D, Babes A, , 2017. The anthelminthic drug praziquantel is a selective agonist of the sensory transient receptor potential melastatin type 8 channel. Toxicol Appl Pharmacol 336: 5565. [Google Scholar]
  25. Mahmud R, Lim YAL, Amir A, , 2017. Cestodes: Tapeworms. Medical Parasitology: A Textbook. Cham, Switzerland: Springer International Publishing, 117–134.
  26. Doenhoff MJ, Cioli D, Utzinger J, , 2008. Praziquantel: mechanisms of action, resistance and new derivatives for schistosomiasis. Curr Opin Infect Dis 21: 659667. [Google Scholar]
  27. Dayan AD, , 2003. Albendazole, mebendazole and praziquantel. Review of non-clinical toxicity and pharmacokinetics. Acta Trop 86: 141159. [Google Scholar]
  28. Whittaker JH, Carlson SA, Jones DE, Brewer MT, , 2017. Molecular mechanisms for anthelmintic resistance in strongyle nematode parasites of veterinary importance. J Vet Pharmacol Ther 40: 105115. [Google Scholar]
  29. Cioli D, Pica-Mattoccia L, Basso A, Guidi A, , 2014. Schistosomiasis control: praziquantel forever? Mol Biochem Parasitol 195: 2329. [Google Scholar]
  30. Vale N, Gouveia MJ, Rinaldi G, Brindley PJ, Gärtner F, Correia da Costa JM, , 2017. Praziquantel for schistosomiasis: single-drug metabolism revisited, mode of action, and resistance. Antimicrob Agents Chemother 61: e02582. [Google Scholar]

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  • Received : 27 Jun 2018
  • Accepted : 30 Jul 2018
  • Published online : 17 Nov 2018

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