1921
Volume 100, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The standard cultivation procedure for requires gas exchange and a microaerophilic atmosphere. A novel system using a commercially available cell culture device (Petaka G3; Celartia Ltd., Powell, OH) was assessed for long-term cultivation of a reference laboratory clone in normal air. Parasite growth during 30 days was similar, or better, in Petaka G3 than that in the standard cultivation method with gas exchange in a CO incubator. The successful cultivation of in the Petaka G3 device suggests that low O content available in hemoglobin and dissolved gas in the blood is sufficient for long-term cultivation. This finding may open the way to novel methods to cultivate and adapt field isolates to conditions with more ease.

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References

  1. Bass CC, 1911. A new conception of immunity. Its application to the cultivation of protozoa and bacteria from the blood and to therapeutic measures. J Am Med Assoc 57: 1534.
    [Google Scholar]
  2. Bass CC, Johns FM, 1912. The cultivation of malarial plasmodia (Plasmodium vivax and Plasmodium falciparum) in vitro. J Exp Med 16: 567579.
    [Google Scholar]
  3. Haynes JD, Diggs CL, Hines FA, Desjardins RE, 1976. Culture of human malaria parasites P. falciparum. Nature 263: 767769.
    [Google Scholar]
  4. Trager W, Jensen JB, 1976. Human malaria parasites in continuous culture. Science 193: 673675.
    [Google Scholar]
  5. Grun JL, Weidanz WP, 1987. Cultivation of Plasmodium falciparum in commercially available sera depleted of natural antibodies reactive with human erythrocytes. J Parasitol 73: 384388.
    [Google Scholar]
  6. Basco LK, 2003. Molecular epidemiology of malaria in Cameroon. XV. Experimental studies on serum substitutes and supplements and alternative culture media for in vitro drug sensitivity assays using fresh isolates of Plasmodium falciparum. Am J Trop Med Hyg 69: 168173.
    [Google Scholar]
  7. Basco LK, 2006. Molecular epidemiology of malaria in Cameroon. XXIII. Experimental studies on serum substitutes and alternative culture media for in vitro drug sensitivity assays using clinical isolates of Plasmodium falciparum. Am J Trop Med Hyg 75: 777782.
    [Google Scholar]
  8. Srivastava K, Singh S, Singh P, Puri SK, 2007. In vitro cultivation of Plasmodium falciparum: studies with modified medium supplemented with Albumax II and various animal sera. Exp Parasitol 116: 171174.
    [Google Scholar]
  9. Zolg JW, Macleod AJ, Dickson IH, Scaife JG, 1982. Plasmodium falciparum: modifications of the in vitro culture conditions improving parasitic yields. J Parasitol 68: 10721080.
    [Google Scholar]
  10. Ferrer J, Rosal MD, Vidal JM, Prats C, Valls J, Herreros EA, Lopez D, Gargallo D, 2008. Effect of the haematocrit layer geometry on Plasmodium falciparum static thin-layer in vitro cultures. Malar J 7: 203.
    [Google Scholar]
  11. Allen RJW, Kirk K, 2010. Plasmodium falciparum culture: the benefits of shaking. Mol Biochem Parasitol 169: 6365.
    [Google Scholar]
  12. Scheibel LW, Ashton SH, Trager W, 1979. Plasmodium falciparum: microaerophilic requirements in human red blood cells. Exp Parasitol 47: 410418.
    [Google Scholar]
  13. Waki S, Miyagami T, Nakazawa S, Igarashi I, Suzuki M, 1987. Maintenance and propagation of human malaria parasites in culture using an ordinary CO2 incubator. Trans R Soc Trop Med Hyg 78: 418.
    [Google Scholar]
  14. Binh VQ, Luty AJF, Kremsner PG, 1997. Differential effects of human serum and cells on the growth of Plasmodium falciparum adapted to serum-free in vitro culture conditions. Am J Trop Med Hyg 57: 594600.
    [Google Scholar]
  15. Haruki K, Kobayashi F, Fujino T, Matsui T, Tsuji M, 2001. Evaluation of Anaeropack® (AnP) type as tools of Plasmodium falciparum cultivation and drug sensitivity tests. Jpn J Trop Med Hyg 29: 365370.
    [Google Scholar]
  16. Pascual A, Basco LK, Baret E, Amalvict R, Travers D, Rogier C, Pradines B, 2011. Use of the atmospheric generators for capnophilic bacteria Genbag-CO2 for the evaluation of in vitro Plasmodium falciparum susceptibility to standard anti-malarial drugs. Malar J 20: 8.
    [Google Scholar]
  17. Grunwald D, Mayol J-F, Ronot X, 2010. Cycle Cellulaire et Cytométrie en Flux. Cachan, France: Lavoisier, 217219.
    [Google Scholar]
  18. Janse CJ, Haghparast A, Sperança MA, Ramesar J, Kroeze H, del Portillo HA, Waters AP, 2003. Malaria parasites lacking eEF1A have a normal S/M phase yet grow more slowly due to a longer G1 phase. Mol Microbiol 50: 15391551.
    [Google Scholar]
  19. Moloney MB, Pawluk AR, Ackland NR, 1990. Plasmodium falciparum growth in deep culture. Trans R Soc Trop Med Hyg 84: 516518.
    [Google Scholar]
  20. Li T, Glushakova S, Zimmerberg J, 2003. A new method for culturing Plasmodium falciparum shows replication at the highest erythrocyte densities. J Infect Dis 187: 159162.
    [Google Scholar]
  21. Preechapornkul P, Chotivanich K, Imwong M, Dondorp AM, Lee SJ, Day NPJ, White NJ, Pukrittayakamee S, 2010. Optimizing the culture of Plasmodium falciparum in hollow fiber bioreactors. Southeast Asian J Trop Med Public Health 41: 761769.
    [Google Scholar]
  22. Dalton JP, Demanga CG, Reiling SJ, Wunderlich J, Eng JWL, Rohrbach P, 2012. Large-scale growth of the Plasmodium falciparum malaria parasite in a wave bioreactor. Int J Parasitol 42: 215220.
    [Google Scholar]
  23. Lazarus MD, Schneider TG, Taraschi TF, 2008. A new model for hemoglobin ingestion and transport by the human malaria parasite Plasmodium falciparum. J Cell Sci 121: 19371949.
    [Google Scholar]
  24. Divo AA, Geary TG, Jensen JB, Ginsburg H, 1985. The mitochondrion of Plasmodium falciparum visualized by rhodamine 123 fluorescence. J Protozool 32: 442446.
    [Google Scholar]
  25. Van Dooren GG, Stimmler LM, McFadden GI, 2006. Metabolic maps and functions of the Plasmodium mitochondrion. FEMS Microbiol Rev 30: 596630.
    [Google Scholar]
  26. Guyton AC, Hall J, 1996. Textbook of Medical Physiology, 9th edition. Philadelphia, PA: W. B. Saunders Company.
    [Google Scholar]
  27. Boron WF, 2003. Transport of oxygen and carbon dioxide in the blood. Boron WF, Boulpaep EL, eds. Medical Physiology. A Cellular and Molecular Approach. Philadelphia, PA: Saunders.
    [Google Scholar]
  28. Krungkrai J, Burat D, Kudan S, Krungkrai S, Prapunwattana P, 1999. Mitochondrial oxygen consumption in asexual and sexual bloodstages of the human malarial parasite, Plasmodium falciparum. Southeast Asian J Trop Med Public Health 30: 636642.
    [Google Scholar]
  29. Briolant S et al., 2007. Influence of oxygen on asexual blood cycle and susceptibility of Plasmodium falciparum to chloroquine: requirement of a standardized in vitro assay. Malar J 6: 44.
    [Google Scholar]
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  • Received : 25 Jun 2018
  • Accepted : 05 Dec 2018
  • Published online : 28 Jan 2019

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