1921
Volume 100, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract.

The standard cultivation procedure for requires gas exchange and a microaerophilic atmosphere. A novel system using a commercially available cell culture device (Petaka G3; Celartia Ltd., Powell, OH) was assessed for long-term cultivation of a reference laboratory clone in normal air. Parasite growth during 30 days was similar, or better, in Petaka G3 than that in the standard cultivation method with gas exchange in a CO incubator. The successful cultivation of in the Petaka G3 device suggests that low O content available in hemoglobin and dissolved gas in the blood is sufficient for long-term cultivation. This finding may open the way to novel methods to cultivate and adapt field isolates to conditions with more ease.

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  • Received : 25 Jun 2018
  • Accepted : 05 Dec 2018
  • Published online : 28 Jan 2019

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