1921
Volume 99, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Cutaneous leishmaniasis is endemic in Salta province, which belongs to the northwest of Argentina. spp. DNA from Giemsa-stained slides of up to 12 years in storage of patients from Salta was characterized through polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP). One hundred smears positive for microscopy, classified in a semiquantitative scale for amastigote density, were analyzed. Also, Leishmanin skin test (LST) results were included. DNA extraction was carried out applying lysis buffer with proteinase K, and then DNA was amplified with ribosomal internal transcribed spacer 1 primers. PCR products were digested with HaeIII enzyme. All PCR-positive smears (74/100) belonged to subgenus. A statistically significant, directly proportional relationship between semiquantitative microscopy and PCR results was detected. All patients had LST-positive results (induration ≥ 5 mm), and the smears of those with smaller induration (LST < 19 mm) gave a higher proportion of positive PCR results. This study determined that smear age did not affect PCR positivity, which allows retrospective analyzes and suggests smears might be useful for molecular complementary diagnosis. Because () is the main circulating species in the study area, determining subgenus in all analyzed samples confirms previous findings. PCR positivity showed statistically significant differences according to semiquantitative microscopy, highlighting the importance of parasite burden in the diagnostic sensitivity of the method. Considering that smears of patients with smaller LST induration were more positive in PCR, a negative smear from patients with positive LST response, but < 19 mm, could actually represent a false-negative result.

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References

  1. Grimaldi G, Tesh RB, , 1993. Leishmaniases of the new world: current concepts and implications for future research. Clin Microbiol Rev 6: 230250. [Google Scholar]
  2. Espinosa OA, Serrano MG, Camargo EP, Teixeira MMG, Shaw JJ, , 2016. An appraisal of the taxonomy and nomenclature of trypanosomatids presently classified as Leishmania and Endotrypanum. Parasitology 145: 430442. [Google Scholar]
  3. Desjeux P, , 2004. Leishmaniasis: current situation and new perspectives. Comp Immunol Microbiol Infect Dis 27: 305318. [Google Scholar]
  4. Salomon OD, Quintana MG, Zaidenberg M, , 2008. Urban distribution of phlebotominae in a cutaneous leishmaniasis focus, Argentina. Mem Inst Oswaldo Cruz 103: 282287. [Google Scholar]
  5. Barrio AB, Parodi CM, Locatelli FM, Mora MC, Basombrío MA, Korenaga M, Hashiguchi Y, Garcia Bustos MF, Gentile A, Marco JD, , 2012. Leishmania infantum and human visceral leishmaniasis, Argentina. Emerg Infect Dis 18: 354355. [Google Scholar]
  6. Frank FM, Fernandez MM, Taranto NJ, Cajal SP, Margni RA, Castro E, Thomaz-Soccol V, Malchiodi E, , 2003. Characterization of human infection by Leishmania spp. in the northwest of Argentina: immune response, double infection with Trypanosoma cruzi and species of Leishmania involved. Parasitology 126: 3139. [Google Scholar]
  7. Marco JD, 2005 Species assignation of Leishmania from human and canine American tegumentary leishmaniasis cases by multilocus enzyme electrophoresis in north Argentina. Am J Trop Med Hyg 72: 606611. [Google Scholar]
  8. Marco JD, Uezato H, Mimori T, Barroso PA, Korenaga M, Nonaka S, Basombrío MA, Taranto NJ, Hashiguchi Y, , 2006. Are cytochrome B gene sequencing and polymorphism-specific polymerase chain reaction as reliable as multilocus enzyme electrophoresis for identifying Leishmania spp. from Argentina? Am J Trop Med Hyg 75: 256260. [Google Scholar]
  9. Marco JD, 2012. Polymorphism-specific PCR enhances the diagnostic performance of American tegumentary leishmaniasis and allows the rapid identification of Leishmania species from Argentina. BMC Infect Dis 12: 191. [Google Scholar]
  10. Ramirez JR, Agudelo S, Muskus C, Alzate JF, Berberich C, Barker D, Velez ID, , 2000. Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitologic diagnosis. J Clin Microbiol 38: 37683773. [Google Scholar]
  11. Motazedian H, Karamian M, Noyes HA, Ardehali S, , 2002. DNA extraction and amplification of Leishmania from archived, Giemsa-stained slides, for the diagnosis of cutaneous leishmaniasis by PCR. Ann Trop Med Parasitol 96: 3134. [Google Scholar]
  12. Schönian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HDFH, Presber W, Jaffe CL, , 2003. PCR diagnosis and characterization of Leishmania in local and imported clinical samples. Diagn Microbiol Infect Dis 47: 349358. [Google Scholar]
  13. Kheirandish F, Chegeni Sharafi A, Kazemi B, Mohebali M, Sarlak A, Tarahi MJ, Holakouee K, Hajaran H, , 2013. Identification of Leishmania species using PCR assay on Giemsa-stained slides prepared from cutaneous leishmaniasis patients. Iran J Parasitol 8: 382388. [Google Scholar]
  14. Arevalo J, 2007. Influence of Leishmania (Viannia) species on the response to antimonial treatment in patients with American tegumentary leishmaniasis. J Infect Dis 195: 18461851. [Google Scholar]
  15. Brustoloni YM, Lima RB, Venâncio da Cunha R, Dorval ME, Teruya Oshiro E, Lyrio de Oliveira AL, Pirmez C, , 2007. Sensitivity and specificity of polymerase chain reaction in Giemsa- stained slides for diagnosis of visceral leishmaniasis in children. Mem Inst Oswaldo Cruz 102: 497500. [Google Scholar]
  16. Kazemi-Rad E, Mohebali M, Hajjaran H, Rezaei S, Mamishi S, , 2008. Diagnosis and characterization of Leishmania species in Giemsa-stained slides by PCR-RFLP. Iran J Public Health 37: 5460. [Google Scholar]
  17. Koarashi Y, 2016. Identification of causative Leishmania species in Giemsa-stained smears prepared from patients with cutaneous leishmaniasis in Peru using PCR-RFLP. Acta Trop 158: 8387. [Google Scholar]
  18. El Tai NO, Osman OF, El Fari M, Presber W, Schönian G, , 2000. Genetic heterogeneity of ribosomal internal transcribed spacer in clinical samples of Leishmania donovani spotted on filter paper as revealed by single-strand conformation polymorphisms and sequencing. Trans R Soc Trop Med Hyg 94: 575579. [Google Scholar]
  19. Krolewiecki AJ, Almazan MC, Quipildor M, Juarez M, Gil JF, Espinosa M, Canabire M, Cajal SP, , 2017. Reappraisal of leishmanin skin test (LST) in the management of American cutaneous leishmaniasis: a retrospective analysis from a reference center in Argentina. PLoS Negl Trop Dis 11: 111. [Google Scholar]
  20. Romero GAS, De Farias Guerra MV, Paes MG, De Oliveira Macêdo V, , 2001. Comparison of cutaneous leishmaniasis due to Leishmania (Viannia) braziliensis and L. (V.) guyanensis in Brazil: therapeutic response to meglumine antimoniate. Am J Trop Med Hyg 65: 456465. [Google Scholar]
  21. Croft SL, , 2001. Monitoring drug resistance in leishmaniasis. Trop Med Int Health 6: 899905. [Google Scholar]
  22. Garcia L, Kindt A, Bermudez H, Llanos-cuentas A, De Doncker S, Arevalo J, Quispe Tintaya KW, Dujardin J, , 2004. Culture-independent species typing of neotropical Leishmania for clinical validation of a PCR-based assay targeting heat shock protein 70 genes. J Clin Microbiol 42: 22942297. [Google Scholar]
  23. Barrio AB, Garcia Bustos MF, Mora MC, Parodi CM, Ramos F, Moreno S, Basombrío MA, , 2009. Identificación por PS-PCR de especies de Leishmania y su correlación con características clínicas, epidemiológicas y terapéuticas en Salta, Argentina. Rev Argentina Salud Pública 1: 3033. [Google Scholar]
  24. Locatelli FM, 2014. The isolation and molecular characterization of Leishmania spp. from patients with American tegumentary leishmaniasis in northwest Argentina. Acta Trop 131: 1621. [Google Scholar]
  25. Chiaramonte MG, Zwirner NW, Caropresi SL, Heredia V, Taranto NJ, Malchiodi EL, , 1996. Estudio de casos de leishmaniasis en la provincia de Salta. Evidencias de infeccion mixta por Trypanosoma cruzi y Leishmania spp. Med (Buenos Aires) 56: 259268. [Google Scholar]
  26. Sosa-Estani S, 1998. Clinical features and diagnosis of mucocutaneous leishmaniasis in patients of an endemic area in Salta. Med (Buenos Aires) 58: 685691. [Google Scholar]
  27. Sosa-Estani S, Segura EL, Salomón OD, Gómez A, Peralta M, Coutada V, Ruiz LM, , 2000. Tegumentary leishmaniasis in northern Argentina: distribution of infection and disease, in three municipalities of Salta, 1990–1992. Rev Soc Bras Med Trop 33: 573582. [Google Scholar]
  28. Quintana MG, Salomón OD, De Grosso MSL, , 2010. Distribution of phlebotomine sand flies (Diptera: Psychodidae) in a primary forest-crop interface, Salta, Argentina. J Med Entomol 47: 10031010. [Google Scholar]
  29. Krolewiecki AJ, 2013. Restricted outbreak of American tegumentary leishmaniasis with high microfocal transmission. Am J Trop Med Hyg 88: 578582. [Google Scholar]
  30. Salomon OD, Sosa-Estani S, Dri L, Donnet M, Galarza R, Recalde H, Tijera A, , 2002. Leishmaniosis teguemtnaria en Las Lomitas, provincia de Formosa, Argentina, 1992–2001. Med (Buenos Aires) 62: 562568. [Google Scholar]
  31. Salomon OD, Orellano PW, Quintana MG, Perez S, Sosa-Estani S, Acardi S, Lamfri M, , 2006. Transmision de la leishmaniasis tegumentaria en la Argentina. Med (Buenos Aires) 66: 211219. [Google Scholar]
  32. Córdoba-Lanús E, Lizarralde De Grosso M, Piñero JE, Valladares B, Salomón OD, , 2006. Natural infection of Lutzomyia neivai with Leishmania spp. in northwestern Argentina. Acta Trop 98: 15. [Google Scholar]
  33. Pita-pereira D, Souza GD, Zwetsch A, Alves CR, Britto C, Rangel EF, , 2009. Short Report: first report of Lutzomyia (Nyssomyia) neivai (Diptera : Psychodidae : Phlebotominae) naturally infected by Leishmania (Viannia) braziliensis in a periurban area of south Brazil using a multiplex polymerase chain reaction assay. Am J Trop Med Hyg 80: 593595. [Google Scholar]
  34. Convit J, Pinardi ME, Rondón AJ, , 1972. Diffuse cutaneous leishmaniasis: a disease due to an immunological defect of the host. Trans R Soc Trop Med Hyg 66: 603610. [Google Scholar]
  35. Maurer-Cecchini A, 2009. Immunological determinants of clinical outcome in Peruvian patients with tegumentary leishmaniasis treated with pentavalent antimonials. Infect Immun 77: 20222029. [Google Scholar]
  36. Weigle KA, de Davalos M, Heredia P, Molineros R, Saravia NG, D’Alessandro A, , 1987. Diagnosis of cutaneous and mucocutaneous leishmaniasis in Colombia: a comparison of seven methods. Am J Trop Med Hyg 36: 489496. [Google Scholar]
  37. Jara M, 2013. Real-time PCR assay for detection and quantification of Leishmania (Viannia) organisms in skin and mucosal lesions: exploratory study of parasite load and clinical parameters. J Clin Microbiol 51: 18261833. [Google Scholar]
  38. Volpini A, Marques M, Lopes dos Santos S, Machado-Coelho G, Mayrink W, Romanha A, , 2006. Leishmania identification by PCR of Giemsa-stained lesion imprint slides stored for up to 36 years. Eur Soc Clin Microbiol Infect Dis 12: 815818. [Google Scholar]
  39. Al-Jawabreh A, Schoenian G, Hamarsheh O, Presber W, , 2006. Clinical diagnosis of cutaneous leishmaniasis: a comparison study between standardized graded direct microscopy and ITS1-PCR of Giemsa-stained smears. Acta Trop 99: 5561. [Google Scholar]
  40. Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL, , 2006. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol 44: 14351439. [Google Scholar]
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  • Received : 16 Mar 2018
  • Accepted : 14 Aug 2018
  • Published online : 24 Sep 2018

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