1921
Volume 99, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The present study aims to develop a method for rapid diagnosis of malaria using loop-mediated isothermal amplification (LAMP) combined with a lateral flow device (LFD). By adding the biotin-labeled and fluorescein amidite-labeled loop primers to the LAMP reaction solution, the end product can be visualized on a LFD. The entire procedure takes approximately 42 minutes to complete, LAMP assay exhibited high sensitivity, as the detection limit was 0.01 pg/μL for all five species. It was demonstrated that all ( = 90) and ( = 56) were positively amplified by LAMP-LFD assay, whereas healthy donor samples ( = 8) were negative. However, not all mixed infections were positive, and other infected nonmalaria samples were negative. Loop-mediated isothermal amplification-LFD represents a robust approach with potential suitability for use in resource-constrained laboratories. We believe that LAMP-LFD has a potential to be developed as point-of-care diagnostic tool in future.

Loading

Article metrics loading...

The graphs shown below represent data from March 2017
/content/journals/10.4269/ajtmh.18-0177
2018-06-25
2019-09-20
Loading full text...

Full text loading...

/deliver/fulltext/14761645/99/3/tpmd180177.html?itemId=/content/journals/10.4269/ajtmh.18-0177&mimeType=html&fmt=ahah

References

  1. WHO, 2017. The World Malaria Report 2017. Geneva, Switzerland: World Health Organization.
  2. Singh B, , 2016. Plasmodium knowlesi: an update. Microbiol Aust 37: 3942. [Google Scholar]
  3. Notomi T, Okayama H, Masubuchi H, Yonekawa T, Watanabe K, Amino N, Hase T, , 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28: E63. [Google Scholar]
  4. Nagamine K, Hase T, Notomi T, , 2002. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16: 223229. [Google Scholar]
  5. Yongkiettrakul S, Jaroenram W, Arunrut N, Chareanchim W, Pannengpetch S, Suebsing R, Kiatpathomchai W, Pornthanakasem W, Yuthavong Y, Kongkasuriyachai D, , 2014. Application of loop-mediated isothermal amplification assay combined with lateral flow dipstick for detection of Plasmodium falciparum and Plasmodium vivax. Parasitol Int 63: 777784. [Google Scholar]
  6. Lau YL, Lai MY, Fong MY, Jelip J, Mahmud R, , 2016. Loop-mediated isothermal amplification assay for identification of five human Plasmodium species in Malaysia. Am J Trop Med Hyg 94: 336339. [Google Scholar]
  7. Han ET, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, Jin L, Takeo S, Tsuboi T, , 2007. Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. J Clin Microbiol 45: 25212528. [Google Scholar]
  8. Li Y, Kumar N, Gopalakrishnan A, Ginocchio C, Manji R, Bythrow M, Lemieux B, Kong H, , 2013. Detection and species identification of malaria parasites by isothermal tHDA amplification directly from human blood without sample preparation. J Mol Diagn 15: 634641. [Google Scholar]
  9. Zhang Y, Yao Y, Du W, Wu K, Xu W, Lin M, Tan H, Li J, , 2017. Development of loop-mediated isothermal amplification with Plasmodium falciparum unique genes for molecular diagnosis of human malaria. Pathog Glob Health 111: 247255. [Google Scholar]
  10. Imai K, 2017. A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION nanopore sequencer. BMC Infect Dis 17: 621. [Google Scholar]
  11. Piera KA, Aziz A, William T, Bell D, González IJ, Barber BE, Anstey NM, Grigg MJ, , 2017. Detection of Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax using loop-mediated isothermal amplification (LAMP) in a co-endemic area in Malaysia. Malar J 16: 29. [Google Scholar]
  12. Patel JC, 2013. Real-time loop-mediated isothermal amplification (RealAmp) for the species-specific identification of Plasmodium vivax. PLoS One 8: e54986. [Google Scholar]
  13. Sattabongkot J, Tsuboi T, Han ET, Bantuchai S, Buates S, , 2014. Loop-mediated isothermal amplification assay for rapid diagnosis of malaria infections in an area of endemicity in Thailand. J Clin Microbiol 52: 14711477. [Google Scholar]
  14. Lalle M, Possenti A, Dubey JP, Pozio E, , 2017. Loop-mediated isothermal amplification-lateral-flow dipstick (LAMP-LFD) to detect Toxoplasma gondii oocyst in ready-to-eat salad. Food Microbiol 70: 137142. [Google Scholar]
  15. Wang Y, Li H, Wang Y, Zhang L, Xu J, Ye C, , 2017. Loop-mediated isothermal amplification label-based gold nanoparticles lateral flow biosensor for detection of Enterococcus faecalis and Staphylococcus aureus. Front Microbiol 8: 192. [Google Scholar]
  16. Foo FP, Chan YY, Mohamed M, Wong WK, Najian N, Lim BH, , 2017. Development of a thermostabilised triplex LAMP assay with dry-reagent four target lateral flow dipstick for detection of Entamoeba histolytica and non-pathogenic Entamoeba spp. Anal Chim Acta 966: 7180. [Google Scholar]
  17. Al-Soud WA, Radstrom P, , 2001. Purification and characterization of PCR inhibitory components in blood cells. J Clin Microbiol 39: 485493. [Google Scholar]
  18. Guthrie R, Susi A, , 1963. A simple phenylalanine method for detecting phenylketonuria in large populations of newborn infants. Pediatrics 32: 338343. [Google Scholar]
http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.18-0177
Loading
/content/journals/10.4269/ajtmh.18-0177
Loading

Data & Media loading...

  • Received : 28 Feb 2018
  • Accepted : 07 May 2018
  • Published online : 25 Jun 2018

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error