Volume 98, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We describe the deployment of a custom-designed molecular diagnostic TaqMan Array Card (TAC) to screen for 31 bacterial, protozoal, and viral etiologies in blood from outbreaks of acute febrile illness in Tanzania during 2015–2017. On outbreaks notified to the Tanzanian Ministry of Health, epidemiologists were dispatched and specimens were collected, transported to a central national laboratory, and tested by TAC within 2 days. This algorithm streamlined investigation, diagnosed a typhoid outbreak, and excluded dozens of other etiologies. This method is usable in-country and may be incorporated into algorithms for diagnosing outbreaks.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Prasad N, Murdoch DR, Reyburn H, Crump JA, 2015. Etiology of severe febrile illness in low- and middle-income countries: a systematic review. PLoS One 10: e0127962.
    [Google Scholar]
  2. Iroh Tam PY, Obaro SK, Storch G, 2016. Challenges in the etiology and diagnosis of acute febrile illness in children in low- and middle-income countries. J Pediatric Infect Dis Soc 5: 190205.
    [Google Scholar]
  3. Balajee SA, Arthur R, Mounts AW, 2016. Global health security: building capacities for early event detection, epidemiologic workforce, and laboratory response. Health Secur 14: 424432.
    [Google Scholar]
  4. Liu J et al., 2016. Development of a taqman array card for acute-febrile-illness outbreak investigation and surveillance of emerging pathogens, including ebola virus. J Clin Microbiol 54: 4958.
    [Google Scholar]
  5. Liu J et al., 2014. Development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study. Lancet Infect Dis 14: 716724.
    [Google Scholar]
  6. Diaz MH et al., 2013. Optimization of multiple pathogen detection using the taqman array card: application for a population-based study of neonatal infection. PLoS One 8: e66183.
    [Google Scholar]
  7. Nga TV et al., 2010. The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens. BMC Infect Dis 10: 125.
    [Google Scholar]
  8. Liu J et al., 2016. Use of quantitative molecular diagnostic methods to identify causes of diarrhoea in children: a reanalysis of the gems case-control study. Lancet 388: 12911301.
    [Google Scholar]

Data & Media loading...

  • Received : 25 Jan 2018
  • Accepted : 20 Feb 2018
  • Published online : 02 Apr 2018
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error