1921
Volume 100, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Microscopic examination of stool samples has been considered to be the “gold standard” for diagnosis of intestinal parasites. Recently, polymerase chain reaction (PCR) has been approved by the World Health Organization as the method of choice for the diagnosis of . Of the 106 stool samples collected from the Esmeraldas and Pichincha provinces of Ecuador, all (100%) were positive for / by light microscopy, whereas using real-time PCR (RT-PCR) DNA amplification, 74 (69.8%) were positive for and only three (2.8%) were positive for . Some 29 (27.4%) samples were negative for the presence of either or , this may be due the presence of , which is morphologically identical to / and not specifically targeted by the RT-PCR used. These results indicate the necessity of reevaluating the epidemiology of amebiasis in Ecuador as the prominent species found are nonpathogenic.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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References

  1. Stanley SL, Jr., 2003. Amoebiasis. Lancet 361: 10251034. [Google Scholar]
  2. Fotedar R, Stark D, Beebe N, Marriott D, Ellis J, Harkness J, , 2007. Laboratory diagnostic techniques for Entamoeba species. Clin Microbiol Rev 20: 511532. [Google Scholar]
  3. Jackson TF, , 1998. Entamoeba histolytica and Entamoeba dispar are distinct species; clinical, epidemiological and serological evidence. Int J Parasitol 28: 181186. [Google Scholar]
  4. Oliveira FM, Neumann E, Gomes MA, Caliari MV, , 2015. Entamoeba dispar: could it be pathogenic. Trop Parasitol 5: 914. [Google Scholar]
  5. Formenti F, Perandin F, Bonafini S, Degani M, Bisoffi Z, , 2015. Evaluation of the new ImmunoCard STAT!® CGE test for the diagnosis of amebiasis [in French]. Bull Soc Pathol Exot 108: 171174. [Google Scholar]
  6. Calderaro A, Gorrini C, Bommezzadri S, Piccolo G, Dettori G, Chezzi C, , 2006. Entamoeba histolytica and Entamoeba dispar: comparison of two PCR assays for diagnosis in a non-endemic setting. Trans R Soc Trop Med Hyg 100: 450457. [Google Scholar]
  7. Gatti S, 2002. Amebic infections due to the Entamoeba histolytica-Entamoeba dispar complex: a study of the incidence in a remote rural area of Ecuador. Am J Trop Med Hyg 67: 123127. [Google Scholar]
  8. Levecke B, Dreesen L, Barrionuevo-Samaniego M, Benitez Ortiz W, Praet N, Brandt J, Dorny P, , 2011. Molecular differentiation of Entamoeba spp. in a rural community of Loja province, south Ecuador. Trans R Soc Trop Med Hyg 105: 737739. [Google Scholar]
  9. Verweij JJ, Oostvogel F, Brienen EA, Nang-Beifubah A, Ziem J, Polderman AM, , 2003. Prevalence of Entamoeba histolytica and Entamoeba dispar in northern Ghana. Trop Med Int Health 8: 11531156. [Google Scholar]
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  • Received : 30 Dec 2017
  • Accepted : 15 Aug 2018

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