Volume 100, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Microscopic examination of stool samples has been considered to be the “gold standard” for diagnosis of intestinal parasites. Recently, polymerase chain reaction (PCR) has been approved by the World Health Organization as the method of choice for the diagnosis of . Of the 106 stool samples collected from the Esmeraldas and Pichincha provinces of Ecuador, all (100%) were positive for / by light microscopy, whereas using real-time PCR (RT-PCR) DNA amplification, 74 (69.8%) were positive for and only three (2.8%) were positive for . Some 29 (27.4%) samples were negative for the presence of either or , this may be due the presence of , which is morphologically identical to / and not specifically targeted by the RT-PCR used. These results indicate the necessity of reevaluating the epidemiology of amebiasis in Ecuador as the prominent species found are nonpathogenic.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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  • Received : 30 Dec 2017
  • Accepted : 15 Aug 2018
  • Published online : 05 Nov 2018
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