1921
Volume 98, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

More than 80% of the global burden of the is contributed by mainly three countries (India, Indonesia, and Pakistan). Reports from last decades have highlighted the occurrence of severe malaria which was earlier considered to be benign. The recent trends of increasing –associated morbidity and mortality emphasizes the need for early and accurate diagnosis of malaria for the timely management of patients. Microscopy is considered a gold standard but needs experienced laboratory technologists. Over the last few years, Polymerase chain reaction (PCR) is being used as a highly sensitive and specific test but it requires expensive equipment which limits its use in the field. Therefore, in the present study, utility of visually improved loop-mediated isothermal amplification (LAMP) for the detection of was evaluated targeting 18SrRNA gene in 145 microscopically confirmed and 20 negative patients. Sensitivity and specificity of LAMP was assessed with respect to microscopy and multiplex nested PCR (nPCR). Results of the LAMP assay was also correlated with rapid diagnostic test, multiplex nPCR and real-time PCR results. Overall, sensitivity and specificity of –specific LAMP compared with microscopy were found to be 100% and 85%, respectively. Furthermore, detection limit for LAMP was found to be 0.8 copies/μL and it was also able to detect three complicated cases of which were missed by microscopy. This study showed a LAMP assay to be a rapid and very sensitive method for the early diagnosis of both complicated and uncomplicated malaria.

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References

  1. World Health Organization, 2017. World Malaria Report 2017. Geneva, Switzerland: WHO.
  2. Sharma S, Aggarwal KC, Deswal S, Raut D, Roy N, Kapoor R, , 2013. The unusual presentation of a usual organism—the changing spectrum of the clinical manifestations of Plasmodium vivax malaria in children: a retrospective study. J Clin Diagn Res 7: 19641967. [Google Scholar]
  3. Tjitra E, Anstey NM, Sugiarto P, Warikar N, Kenangalem E, Karyana M, Lampah DA, Price RN, , 2008. Multidrug-resistant Plasmodium vivax associated with severe and fatal malaria: a prospective study in Papua, Indonesia. PLoS Med 5: e128. [Google Scholar]
  4. Kochar DK, Das A, Kochar SK, Saxena V, Sirohi P, Garg S, Kochar A, Khatri MP, Gupta V, , 2009. Severe Plasmodium vivax malaria: a report on serial cases from Bikaner in northwestern India. Am J Trop Med Hyg 80: 194198. [Google Scholar]
  5. Fabbri C, de Cássia Mascarenhas-Netto R, Lalwani P, Melo GC, Magalhães BML, Alexandre MAA, Lacerda MVG, Lima ES, , 2013. Lipid peroxidation and antioxidant enzymes activity in Plasmodium vivax malaria patients evolving with cholestatic jaundice. Malar J 12: 315. [Google Scholar]
  6. Ketema T, Bacha K, , 2013. Plasmodium vivax associated severe malaria complications among children in some malaria endemic areas of Ethiopia. BMC Public Health 13: 637. [Google Scholar]
  7. Kaushik JS, Gomber S, Dewan P, , 2012. Clinical and epidemiological profiles of severe malaria in children from Delhi, India. J Health Popul Nutr 30: 113116. [Google Scholar]
  8. Sharma R, Gohain S, Chandra J, Kumar V, Chopra A, Chatterjee S, Aneja S, Dutta AK, , 2012. Plasmodium vivax malaria admissions and risk of mortality in a tertiary-care children’s hospital in north India. Paediatr Int Child Health 32: 152157. [Google Scholar]
  9. Naha K, Dasari S, Prabhu M, , 2012. Spectrum of complications associated with Plasmodium vivax infection in a tertiary hospital in south-western India. Asian Pac J Trop Med 5: 7982. [Google Scholar]
  10. Chotivanich K, Silamut K, Day NPJ, , 2007. Laboratory diagnosis of malaria infection—a short review of methods. NZJ Med Lab Sci 61: 47. [Google Scholar]
  11. Lau Y-L, Lai M-Y, Fong M-Y, Jelip J, Mahmud R, , 2016. Loop-mediated isothermal amplification assay for identification of five human Plasmodium species in Malaysia. Am J Trop Med Hyg 94: 336339. [Google Scholar]
  12. WHO, 2017. Malaria Rapid Diagnostic Test Performance, Vol. 7. Results of WHO product testing of malaria RDTs: round 7 (2015–2016). Available at: http://www.who.int/malaria/publications/atoz/978924151268/en/.
  13. Britton S, Cheng Q, McCarthy JS, , 2016. Novel molecular diagnostic tools for malaria elimination: a review of options from the point of view of high-throughput and applicability in resource limited settings. Malar J 15: 88. [Google Scholar]
  14. Perandin F, 2004. Development of a real-time PCR assay for detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for routine clinical diagnosis. J Clin Microbiol 42: 12141219. [Google Scholar]
  15. Piera KA, Aziz A, William T, Bell D, González IJ, Barber BE, Anstey NM, Grigg MJ, , 2017. Detection of Plasmodium knowlesi, Plasmodium falciparum and Plasmodium vivax using loop-mediated isothermal amplification (LAMP) in a co-endemic area in Malaysia. Malar J 16: 29. [Google Scholar]
  16. Snounou G, Viriyakosol S, Zhu XP, Jarra W, Pinheiro L, do Rosario VE, Thaithong S, Brown KN, , 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61: 315320. [Google Scholar]
  17. Hänscheid T, Grobusch MP, , 2002. How useful is PCR in the diagnosis of malaria? Trends Parasitol 18: 395398. [Google Scholar]
  18. Singh R, Singh DP, Savargaonkar D, Singh OP, Bhatt RM, Valecha N, , 2017. Evaluation of SYBR green I based visual loop-mediated isothermal amplification (LAMP) assay for genus and species-specific diagnosis of malaria in P. vivax and P. falciparum endemic regions. J Vector Borne Dis 54: 5460. [Google Scholar]
  19. Patel JC, 2014. Field evaluation of a real-time fluorescence loop-mediated isothermal amplification assay, RealAmp, for the diagnosis of malaria in Thailand and India. J Infect Dis 210: 11801187. [Google Scholar]
  20. Surabattula R, Vejandla MP, Mallepaddi PC, Faulstich K, Polavarapu R, , 2013. Simple, rapid, inexpensive platform for the diagnosis of malaria by loop mediated isothermal amplification (LAMP). Exp Parasitol 134: 333340. [Google Scholar]
  21. World Health Organization, 2012. Management of Severe Malaria, 3rd edition. Geneva, Switzerland: WHO
  22. Singh B, Bobogare A, Cox-Singh J, Snounou G, Abdullah MS, Rahman HA, , 1999. A genus- and species-specific nested polymerase chain reaction malaria detection assay for epidemiologic studies. Am J Trop Med Hyg 60: 687692. [Google Scholar]
  23. Han E-T, Watanabe R, Sattabongkot J, Khuntirat B, Sirichaisinthop J, Iriko H, Jin L, Takeo S, Tsuboi T, , 2007. Detection of four Plasmodium species by genus- and species-specific loop-mediated isothermal amplification for clinical diagnosis. J Clin Microbiol 45: 25212528. [Google Scholar]
  24. MedCalc, 2015. MedCalc Statistical Software. Version 15.6.1. Ostend, Belgium: MedCalc Software bvba. Available at: https://www.medcalc.org/. Accessed October 5, 2017.
  25. World Health Organization, 2017. World Malaria Report 2017. Geneva, Switzerland: WHO.
  26. Lucchi NW, 2016. Evaluation of the illumigene malaria LAMP: a robust molecular diagnostic tool for malaria parasites. Sci Rep 6: 36808. [Google Scholar]
  27. Perera RS, Ding XC, Tully F, Oliver J, Bright N, Bell D, Chiodini PL, Gonzalez IJ, Polley SD, , 2017. Development and clinical performance of high throughput loop-mediated isothermal amplification for detection of malaria. PLoS One 12: e0171126. [Google Scholar]
  28. Tao Z-Y, 2011. Adaptation of a visualized loop-mediated isothermal amplification technique for field detection of Plasmodium vivax infection. Parasit Vectors 4: 115. [Google Scholar]
  29. Singh R, Savargaonkar D, Bhatt R, Valecha N, , 2013. Rapid detection of Plasmodium vivax in saliva and blood using loop mediated isothermal amplification (LAMP) assay. J Infect 67: 245247. [Google Scholar]
  30. Ghayour Najafabadi Z, Oormazdi H, Akhlaghi L, Meamar AR, Nateghpour M, Farivar L, Razmjou E, , 2014. Detection of Plasmodium vivax and Plasmodium falciparum DNA in human saliva and urine: loop-mediated isothermal amplification for malaria diagnosis. Acta Trop 136: 4449. [Google Scholar]
  31. Valecha N, 2009. Histopathology of fatal respiratory distress caused by Plasmodium vivax malaria. Am J Trop Med Hyg 81: 758762. [Google Scholar]
  32. Mehndiratta S, Rajeshwari K, Dubey AP, , 2013. Multiple-organ dysfunction in a case of Plasmodium vivax malaria. J Vector Borne Dis 50: 7173. [Google Scholar]
  33. Lee H-J, Baek J-H, Chae M-H, Joo H, Lee J-S, Chung M-H, Park Y-K, Kim J-T, , 2013. A case of vivax malaria complicated by adult respiratory distress syndrome and successful management with extracorporeal membrane oxygenation. Korean J Parasitol 51: 551555. [Google Scholar]
  34. Leal-Santos FA, Silva SBR, Crepaldi NP, Nery AF, Martin TOG, Alves-Junior ER, Fontes CJF, , 2013. Altered platelet indices as potential markers of severe and complicated malaria caused by Plasmodium vivax: a cross-sectional descriptive study. Malar J 12: 462. [Google Scholar]
  35. Dondorp AM, 2005. Estimation of the total parasite biomass in acute falciparum malaria from plasma PfHRP2. PLoS Med 2: e204. [Google Scholar]
  36. Hendriksen ICE, 2012. Diagnosing severe falciparum malaria in parasitaemic African children: a prospective evaluation of plasma PfHRP2 measurement. PLoS Med 9: e1001297. [Google Scholar]
  37. Barber BE, William T, Grigg MJ, Parameswaran U, Piera KA, Price RN, Yeo TW, Anstey NM, , 2015. Parasite biomass-related inflammation, endothelial activation, microvascular dysfunction and disease severity in vivax malaria. PLoS Pathog 11: e1004558. [Google Scholar]
  38. Ochola LB, Vounatsou P, Smith T, Mabaso ML, Newton CR, , 2006. The reliability of diagnostic techniques in the diagnosis and management of malaria in the absence of a gold standard. Lancet Infect Dis 6: 582588. [Google Scholar]
  39. Murray CK, Gasser RA, Magill AJ, Miller RS, , 2008. Update on rapid diagnostic testing for malaria. Clin Microbiol Rev 21: 97110. [Google Scholar]
  40. Sattabongkot J, Tsuboi T, Han E-T, Bantuchai S, Buates S, , 2014. Loop-mediated isothermal amplification assay for rapid diagnosis of malaria infections in an area of endemicity in Thailand. J Clin Microbiol 52: 14711477. [Google Scholar]
  41. Singh N, Shukla MM, Shukla MK, Mehra RK, Sharma S, Bharti PK, Singh MP, Singh A, Gunasekar A, , 2010. Field and laboratory comparative evaluation of rapid malaria diagnostic tests versus traditional and molecular techniques in India. Malar J 9: 191. [Google Scholar]
  42. Ranjan P, Ghoshal U, , 2016. Utility of nested polymerase chain reaction over the microscopy and immuno-chromatographic test in the detection of Plasmodium species and their clinical spectrum. Parasitol Res 115: 33753385. [Google Scholar]
  43. Elahi R, Mohon AN, Khan WA, Haque R, Alam MS, , 2013. Performance of a HRP-2/pLDH based rapid diagnostic test at the Bangladesh-India-Myanmar border areas for diagnosis of clinical malaria. Malar J 12: 378. [Google Scholar]
  44. Kochar DK, 2010. Clinical features of children hospitalized with malaria—a study from Bikaner, northwest India. Am J Trop Med Hyg 83: 981989. [Google Scholar]
  45. Poon LLM, 2006. Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem 52: 303306. [Google Scholar]
  46. Sriworarat C, Phumee A, Mungthin M, Leelayoova S, Siriyasatien P, , 2015. Development of loop-mediated isothermal amplification (LAMP) for simple detection of Leishmania infection. Parasit Vectors 8: 591. [Google Scholar]
  47. Yang H-W, 2013. Loop-mediated isothermal amplification targeting 18S ribosomal DNA for rapid detection of Acanthamoeba. Korean J Parasitol 51: 269277. [Google Scholar]
  48. Mewara A, Khurana S, Yoonus S, Megha K, Tanwar P, Gupta A, Sehgal R, , 2017. Evaluation of loop-mediated isothermal amplification assay for rapid diagnosis of Acanthamoeba keratitis. Indian J Med Microbiol 35: 9094. [Google Scholar]
  49. Hopkins H, 2013. Highly sensitive detection of malaria parasitemia in a malaria-endemic setting: performance of a new loop-mediated isothermal amplification kit in a remote clinic in Uganda. J Infect Dis 208: 645652. [Google Scholar]
  50. Polley SD, Mori Y, Watson J, Perkins MD, González IJ, Notomi T, Chiodini PL, Sutherland CJ, , 2010. Mitochondrial DNA targets increase sensitivity of malaria detection using loop-mediated isothermal amplification. J Clin Microbiol 48: 28662871. [Google Scholar]
  51. Yamamura M, Makimura K, Ota Y, , 2009. Evaluation of a new rapid molecular diagnostic system for Plasmodium falciparum combined with DNA filter paper, loop-mediated isothermal amplification, and melting curve analysis. Jpn J Infect Dis 62: 2025. [Google Scholar]
  52. Lucchi NW, Ljolje D, Silva-Flannery L, Udhayakumar V, , 2016. Use of malachite green-loop mediated isothermal amplification for detection of Plasmodium spp. parasites. PLoS One 11: e0151437. [Google Scholar]
  53. Mugambi RM, Agola EL, Mwangi IN, Kinyua J, Shiraho EA, Mkoji GM, , 2015. Development and evaluation of a loop mediated isothermal amplification (LAMP) technique for the detection of hookworm (Necator americanus) infection in fecal samples. Parasit Vectors 8: 574. [Google Scholar]
  54. Shiraho EA, Eric AL, Mwangi IN, Maina GM, Kinuthia JM, Mutuku MW, Mugambi RM, Mwandi JM, Mkoji GM, , 2016. Development of a loop mediated isothermal amplification for diagnosis of Ascaris lumbricoides in fecal samples. J Parasitol Res 2016: 7376207. [Google Scholar]
  55. Verma S, Avishek K, Sharma V, Negi NS, Ramesh V, Salotra P, , 2013. Application of loop-mediated isothermal amplification assay for the sensitive and rapid diagnosis of visceral leishmaniasis and post-kala-azar dermal leishmaniasis. Diagn Microbiol Infect Dis 75: 390395. [Google Scholar]
  56. Kong Q-M, Lu S-H, Tong Q-B, Lou D, Chen R, Zheng B, Kumagai T, Wen L-Y, Ohta N, Zhou X-N, , 2012. Loop-mediated isothermal amplification (LAMP): early detection of Toxoplasma gondii infection in mice. Parasit Vectors 5: 2. [Google Scholar]
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  • Received : 04 Nov 2017
  • Accepted : 27 Jan 2018
  • Published online : 19 Mar 2018

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