Volume 100, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The potential for transfusion transmission of dengue virus (DENV), chikungunya virus (CHIKV), and Zika virus (ZIKV) has raised concerns about the safety of the blood supply in endemic areas. In this study, nucleic acid testing (NAT) for ZIKV, DENV, and CHIKV RNA was performed in asymptomatic blood donor samples in the city of Campinas, located in the southeast region of Brazil (1962 in 2015 and 1775 in 2016). The prevalence of reactive NAT was 0.15% in 2015 and 0.62% in 2016 for dengue, 0.05% in 2015 and 0.17% in 2016 for Zika, and 0% in both years for chikungunya. These results demonstrate the weakness of the clinical interview in screening these blood donors. Furthermore, positivity for ZIKV was detected in March 2015, 1 year before the first reported cases in the region. These data attest the feasibility of using donor samples held in library as a tool for retrospective epidemiological evaluation, which is particularly interesting considering emerging pathogens, for which data on their spread and penetrance are initially scarce.


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  1. World Health Organization Zika Virus Research Agenda, 2016. WHO Reference Number: WHO/ZIKV/PHR/16.1. Geneva, Switzerland: World Health Organization [Google Scholar]
  2. Cao B, Diamond MS, Mysorekar IU, , 2017. Maternal-fetal transmission of Zika virus: routes and signals for infection. J Interferon Cytokine Res 37: 287294. [Google Scholar]
  3. Turmel JM, Abgueguen P, Hubert B, Vandamme YM, Maquart M, Le Guillou-Guillemette H, Leparc-Goffart I, , 2016. Late sexual transmission of Zika virus related to persistence in the semen. Lancet 387: 2501. [Google Scholar]
  4. Folkers KM, Caplan AL, Igel LH, , 2017. Zika, sexual transmission and prudent public health policy. Public Health 148: 6668. [Google Scholar]
  5. Musso D, Nhan T, Robin E, Roche C, Bierlaire D, Zisou K, Shan Yan A, Cao-Lormeau VM, Broult J, , 2014. Potential for Zika virus transmission through blood transfusion demonstrated during an outbreak in French Polynesia, November 2013 to February 2014. Euro Surveill 19: 20761. [Google Scholar]
  6. Motta IJF, 2016. Evidence for transmission of Zika virus by platelet transfusion. New Engl J Med 375: 11. [Google Scholar]
  7. Barjas-Castro ML, 2016. Probable transfusion-trasmitted Zika virus in Brazil. Transfusion 56: 16841688. [Google Scholar]
  8. Katz LM, Rossmann SN, , 2017. Zika and the blood suply. A work in progress. Arch Pathol Lab Med 141: 8592. [Google Scholar]
  9. Municipal Health Department of Campinas, 2016. Epidemiological Bulletin, November 24th, Campinas. Available at: http://www.saude.campinas.sp.gov.br/vigilancia/informes/2016/Informe_Epidemiologico_Arboviroses_24_novembro_2016.pdf. Acessed June 14, 2018.
  10. Matos D, 2016. Probable and possible transfusion-transmitted dengue associated with NS1 antigen-negative but RNA confirmed-positive red blood cells. Transfusion 56: 215222. [Google Scholar]
  11. Stramer SL, 2012. Dengue viremia in blood donors identified by RNA and detection of dengue transfusion transmission during the 2007 dengue outbreak in Puerto Rico. Transfusion 52: 16571666. [Google Scholar]
  12. Levi JE, Nishiya A, Félix AC, Salles NA, Sampaio LR, Hangai F, Sabino EC, Mendrone A, Jr., 2015. Real-time symptomatic case of transfusion-transmitted dengue. Transfusion 55: 961964. [Google Scholar]
  13. Júnior DL, Covas DT, Bianco C, Sabino E, Marques JFC, Levi JE, Salles NA, Gonçales NSL, Kashima S, , 2014. Official comunique: chikungunya virus–press release of the Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular regarding the safety of transfusions and transplants. Rev Bras Hematol Hemoter 36: 309310. [Google Scholar]
  14. CDC-Centers for Disease Control and Prevention, 2016. Zika Virus. Available at: https://www.cdc.gov/zika/. Accessed May 20, 2016.
  15. Kuehnert MJ, 2016. Screening of blood donations for Zika virus infection—Puerto Rico, April 3–June 11, 2016. MMWR Morb Mortal Wkly Rep 65: 627628. [Google Scholar]
  16. Marano G, Pupella S, Vaglio S, Liumbruno GM, Grazzini G, , 2016. Zika vírus and the never-ending story of emerging pathogens and transfusion medicine. Blood Transfus 14: 95100. [Google Scholar]
  17. Añez G, Jiang Z, Heisey DA, Kerby S, Rios M, Chikungunya Virus Collaborative Study Group; , 2015. Collaborative study for the characterization of a chikungunya virus RNA reference reagent for use in nucleic acid testing. Vox Sang 109: 312318. [Google Scholar]
  18. Baylis SA, Hanschmann KO, Schnierle BS, Trösemeier JH, Blümel J, Zika Virus Collaborative Study Group; , 2017. Harmonization of nucleic acid testing for Zika virus: development of the 1st World Health Organization International Standard. Transfusion 57: 748761. [Google Scholar]
  19. Stone M, 2017. Relative analytical sensitivity of donor NAT screening and diagnostic real-time PCR assays for detection of Zika virus RNA. Transfusion 57:734747. [Google Scholar]
  20. Simmons G, 2016. High incidence of chikungunya virus and frequency of viremic blood donations during epidemic, Puerto Rico, USA, 2014. Emerg Infect Dis 22: 12211228. [Google Scholar]
  21. Busch MP, For the International Component of the NHLBI Recipient Epidemiology and Donor Evaluation Study-III (REDS-III) , 2016. Duration of dengue viremia in blood donors and relationships between donor viremia, infection incidence and clinical case reports during a large epidemic. J Infect Dis 214: 4954. [Google Scholar]
  22. Marks P, Peterson L, , 2017. Decision making in the face of uncertainty: the challenge of emerging infectious diseases. Transfusion 57: 723728. [Google Scholar]
  23. Galel S, 2017. First Zika positive donations in the continental United States. Transfusion 57: 762769. [Google Scholar]
  24. Williamson P, 2017. First cases of Zika virus infected US blood donors outside states with areas of active transmission. Transfusion 57: 770778. [Google Scholar]
  25. Sabino E, Loureiro P, Lopes ME, Capuani L, McClure C, Chowdhury D, 2016. Transfusion-transmitted dengue associated clinical symptoms during the 2012 epidemic in Brazil. J Infect Dis 213: 694702. [Google Scholar]
  26. Silva MMO, 2016. Accuracy of dengue reporting by National Surveillance System, Brazil. Emerg Infect Dis 22: 336339. [Google Scholar]
  27. Lanteri MC, Kleinman SH, Glynn SA, Musso D, Hoots WK, Custer BC, Sabino ES, Bush MP, , 2016. Zika virus: a new threat to the safety of the blood supply with worldwide impact and implications. Transfusion 56: 19071914. [Google Scholar]
  28. Brasil. Ministério da Saúde. Portal Dengue Chikungunya e Zika, 2015. Epidemiological Bulletin: Monitoring Cases of Dengue Fever, Chikungunya Fever and Zika Virus fever up to Epidemiological Week 45, 2015. Available at: http://portalarquivos2.saude.gov.br/images/pdf/2015/novembro/26/2015-dengue-SE45.pdf. Accessed June14, 2018.
  29. Zanluca C, Melo VC, Mosimann AL, Santos GI, Santos CN, Luz K, , 2015. First report of autochthonous transmission of Zika virus in Brazil. Mem Inst Oswaldo Cruz 110: 569572. [Google Scholar]
  30. Lowe R, Barcellos C, Brasil P, Cruz OG, Honório NA, Kuper H, Carvalho MS, , 2018. The Zika virus epidemic in Brazil: from discovery to future implications. Int J Environ Res Public Health 9: 15. [Google Scholar]
  31. Department of Health Surveillance, Municipal Health Department, Campinas, 2016. Technical Report: Zika Virus. Available at: http://www.saude.campinas.sp.gov.br/vigilancia/informes/2016/Informe_Tecnico_01_Zika_Virus_jun_2016.pdf. Accessed September 22, 2018.
  32. Dıaz-Quinonez JA, Lopez-Martınez I, Torres-Longoria B, Vazquez-Pichardo M, Cruz-Ramırez E, Ramırez-Gonzalez JE, Ruiz-Matus C, Kuri-Morales P, , 2016. Evidence of the presence of the Zika virus in Mexico since early 2015. Virus Genes 52: 855857. [Google Scholar]
  33. Lednicky J, 2016. Zika virus outbreak in Haiti in 2014: molecular and clinical data. PLoS Negl Trop Dis 10: e0004687. [Google Scholar]

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  • Received : 30 Oct 2017
  • Accepted : 24 Sep 2018
  • Published online : 05 Nov 2018

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