Volume 99, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



spp. are emerging infectious pathogens, especially in the Americas. Although is primarily a parasite of dogs, polymerase chain reaction-confirmed human infections have been reported from Mexico, Venezuela, and Costa Rica. This study reports the presence of DNA in 13.7% of 205 dogs from urban areas in Peru and of those, five were analyzed for phylogenetic variation using the Tandem Repeat Protein 36 (TRP36) gene. The use of the TRP36 gene for such analysis was validated against 16S rRNA and heat shock protein genes using Shannon’s entropy bioinformatic approach. When compared with other strains previously reported, three unique and novel strains were detected. In addition, the TRP36 amino acid tandem repeat sequences of the Peruvian strains share close similarity to an strain detected from four human blood bank samples in Costa Rica. This study reports for the first time domestic dogs infected with strains closely related to a zoonotic strain, which may be of public health concern as dogs can be chronically infected with this pathogen.


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  1. Dantas-Torres F, Chomel BB, Otranto D, , 2012. Ticks and tick-borne diseases: a one health perspective. Trends Parasitol 28: 437446. [Google Scholar]
  2. Eremeeva ME, 2007. Bacteremia, fever, and splenomegaly caused by a newly recognized Bartonella species. N Engl J Med 356: 23812387. [Google Scholar]
  3. Arraga-Alvarado CM, Qurollo BA, Parra OC, Berrueta MA, Hegarty BC, Breitschwerdt EB, , 2014. Molecular evidence of Anaplasma platys infection in two women from Venezuela. Am J Trop Med Hyg 91: 11611165. [Google Scholar]
  4. Allison RW, Little SE, , 2013. Diagnosis of rickettsial diseases in dogs and cats. Vet Clin Pathol 42: 127144. [Google Scholar]
  5. de Castro MB, Machado RZ, de Aquino LP, Alessi AC, Costa MT, , 2004. Experimental acute canine monocytic ehrlichiosis: clinicopathological and immunopathological findings. Vet Parasitol 119: 7386. [Google Scholar]
  6. Vinasco J, Li O, Alvarado A, Diaz D, Hoyos L, Tabachi L, Sirigireddy K, Ferguson C, Moro MH, , 2007. Molecular evidence of a new strain of Ehrlichia canis from South America. J Clin Microbiol 45: 27162719. [Google Scholar]
  7. Gray J, Dantas-Torres F, Estrada-Pena A, Levin M, , 2013. Systematics and ecology of the brown dog tick, Rhipicephalus sanguineus. Ticks Tick Borne Dis 4: 171180. [Google Scholar]
  8. Uspensky I, Ioffe-Uspensky I, , 2002. The dog factor in brown dog tick Rhipicephalus sanguineus (Acari: Ixodidae) infestations in and near human dwellings. Int J Med Microbiol 291 (Suppl 33): 156163. [Google Scholar]
  9. Eiras DF, Craviotto MB, Vezzani D, Eyal O, Baneth G, , 2013. First description of natural Ehrlichia canis and Anaplasma platys infections in dogs from Argentina. Comp Immunol Microbiol Infect Dis 36: 169173. [Google Scholar]
  10. Vieira RF, 2011. Ehrlichiosis in Brazil. Rev Bras Parasitol Vet 20: 112. [Google Scholar]
  11. McCown ME, Monterroso VH, Cardona W, , 2014. Surveillance for Ehrlichia canis, Anaplasma phagocytophilum, Borrelia burgdorferi, and Dirofilaria immitis in dogs from three cities in Colombia. J Spec Oper Med 14: 8690. [Google Scholar]
  12. Diniz PP, 2013. Infection of domestic dogs in Peru by zoonotic Bartonella species: a cross-sectional prevalence study of 219 asymptomatic dogs. PLoS Negl Trop Dis 7: e2393. [Google Scholar]
  13. Huerto-Medina E, Damaso-Mata B, , 2015. Factors associated with Ehrlichia canis infection in dogs infested with ticks from Huanuco, Peru. Rev Peru Med Exp Salud Publica 32: 756760. [Google Scholar]
  14. Diniz PPVP, Schwartz DS, De Morais HSA, Breitschwerdt EB, , 2007. Surveillance for zoonotic vector-borne infections using sick dogs from southeastern Brazil. Vector Borne Zoonotic Dis 7: 689697. [Google Scholar]
  15. Perez M, Rikihisa Y, Wen B, , 1996. Ehrlichia canis-like agent isolated from a man in Venezuela: antigenic and genetic characterization. J Clin Microbiol 34: 21332139. [Google Scholar]
  16. Unver A, Perez M, Orellana N, Huang H, Rikihisa Y, , 2001. Molecular and antigenic comparison of Ehrlichia canis isolates from dogs, ticks, and a human in Venezuela. J Clin Microbiol 39: 27882793. [Google Scholar]
  17. Moro PL, Shah J, Li O, Gilman RH, Harris N, Moro MH, , 2009. Short report: serologic evidence of human ehrlichiosis in Peru. Am J Trop Med Hyg 80: 242244. [Google Scholar]
  18. Aguiar DM, 2013. Genetic diversity of Ehrlichia canis in Brazil. Vet Microbiol 164: 315321. [Google Scholar]
  19. Harrus S, Waner T, , 2011. Diagnosis of canine monocytotropic ehrlichiosis (Ehrlichia canis): an overview. Vet J 187: 292296. [Google Scholar]
  20. Aguiar DM, Zhang X, Braga IA, Taques II, McBride JW, , 2016. Detection of genotype-specific Ehrlichia canis exposure in Brazilian dogs by TRP36 peptide ELISA. Ticks Tick Borne Dis 7: 142145. [Google Scholar]
  21. Aguiar DM, 2014. A novel Ehrlichia genotype strain distinguished by the TRP36 gene naturally infects cattle in Brazil and causes clinical manifestations associated with ehrlichiosis. Ticks Tick Borne Dis 5: 537544. [Google Scholar]
  22. Bouza-Mora L, Dolz G, Solorzano-Morales A, Romero-Zuniga JJ, Salazar-Sanchez L, Labruna MB, Aguiar DM, , 2017. Novel genotype of Ehrlichia canis detected in samples of human blood bank donors in Costa Rica. Ticks Tick Borne Dis 8: 3640. [Google Scholar]
  23. Diniz PP, 2010. High prevalence of tick-borne pathogens in dogs from an Indian reservation in northeastern Arizona. Vector Borne Zoonotic Dis 10: 117123. [Google Scholar]
  24. INEI, 2007. Instituto Nacional de Estadística e Informática, Censo Nationales 2007. Lima, Peru: Presidencia del Consejo de Ministros.
  25. Barber RM, 2010. Evaluation of brain tissue or cerebrospinal fluid with broadly reactive polymerase chain reaction for Ehrlichia, Anaplasma, spotted fever group Rickettsia, Bartonella, and Borrelia species in canine neurological diseases (109 cases). J Vet Intern Med 24: 372378. [Google Scholar]
  26. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, , 1990. Basic local alignment search tool. J Mol Biol 215: 403410. [Google Scholar]
  27. Kimura M, , 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16: 111120. [Google Scholar]
  28. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S, , 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 27312739. [Google Scholar]
  29. Edgar RC, , 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 17921797. [Google Scholar]
  30. Waterhouse AM, Procter JB, Martin DM, Clamp M, Barton GJ, , 2009. Jalview version 2—a multiple sequence alignment editor and analysis workbench. Bioinformatics 25: 11891191. [Google Scholar]
  31. Shenkin PS, Erman B, Mastrandrea LD, , 1991. Information-theoretical entropy as a measure of sequence variability. Proteins 11: 297313. [Google Scholar]
  32. Jari Oksanen 2017. Ordination Methods, Diversity Analysis and Other Functions for Community and Vegetation Ecologists. Available at: https://cran.r-project.org/web/packages/vegan/index.html. Accessed June 1, 2017.
  33. Wickham H, , 2016. Use R! ggplot2: Elegant Graphics for Data Analysis. New York, NY: Springer.
  34. R Core Team, 2017. R: A language and Environment for Statistical Computing. Available at: http://www.R-project.org. Accessed June 1, 2017.
  35. Perez M, Bodor M, Zhang C, Xiong Q, Rikihisa Y, , 2006. Human infection with Ehrlichia canis accompanied by clinical signs in Venezuela. Ann N Y Acad Sci 1078: 110117. [Google Scholar]
  36. Bronson E, Emmons LH, Murray S, Dubovi EJ, Deem SL, , 2008. Serosurvey of pathogens in domestic dogs on the border of Noel Kempff Mercado National Park, Bolivia. J Zoo Wildl Med 39: 2836. [Google Scholar]
  37. Lopez J, Abarca K, Mundaca MI, Caballero C, Valiente-Echeverria F, , 2012. Molecular identification of Ehrlichia canis in a dog from Arica, Chile. Rev Chilena Infectol 29: 527530. [Google Scholar]
  38. McCown ME, Opel T, Grzeszak B, , 2013. Vector-borne disease surveillance in Puerto Rico: pathogen prevalence rates in canines? Implications for public health and the U.S. Military? Applying the one health concept. J Spec Oper Med 13: 5963. [Google Scholar]
  39. Barbet AF, Bekker CP, Dasch GA, Palmer GH, Ray SC, Rikihisa Y, Rurangirwa FR, , 2001. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and ‘HGE agent’ as subjective synonyms of Ehrlichia phagocytophila. Int J Syst Evol Microbiol 51: 21452165. [Google Scholar]
  40. Walker DH, Dumler JS, , 1996. Emergence of the ehrlichioses as human health problems. Emerg Infect Dis 2: 1829. [Google Scholar]
  41. Sumner JW, Nicholson WL, Massung RF, , 1997. PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species. J Clin Microbiol 35: 20872092. [Google Scholar]
  42. Hsieh YC, Lee CC, Tsang CL, Chung YT, , 2010. Detection and characterization of four novel genotypes of Ehrlichia canis from dogs. Vet Microbiol 146: 7075. [Google Scholar]
  43. McBride JW, Doyle CK, Zhang X, Cardenas AM, Popov VL, Nethery KA, Woods ME, , 2007. Identification of a glycosylated Ehrlichia canis 19-kilodalton major immunoreactive protein with a species-specific serine-rich glycopeptide epitope. Infect Immun 75: 7482. [Google Scholar]
  44. Cárdenas AM, Doyle CK, Zhang X, Nethery K, Corstvet RE, Walker DH, McBride JW, , 2007. Enzyme-linked immunosorbent assay with conserved immunoreactive glycoproteins gp36 and gp19 has enhanced sensitivity and provides species-specific immunodiagnosis of Ehrlichia canis infection. Clin Vaccine Immunol 14: 123128. [Google Scholar]
  45. Yu X-j, McBride JW, Walker DH, , 2007. Restriction and expansion of Ehrlichia strain diversity. Vet Parasitol 143: 337346. [Google Scholar]

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  • Received : 05 Oct 2017
  • Accepted : 19 Apr 2018
  • Published online : 25 Jun 2018

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