Volume 98, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Cutaneous leishmaniasis (CL) is diagnosed mainly by light microscopy of smears made using lesion material. Histopathology is usually done in atypical presentations or when lesion smears are negative. Tissue impression smears (TIS) made from skin biopsy specimens were compared with histopathology for the diagnosis of CL. Out of the 111 patients included, 83 (74.8%) were positive by either methods. The TIS was positive in 70.3% whereas histopathology was positive in 56.8% of patients. Tissue impression smears can be used as a supplementary diagnostic test that gives sensitive and rapid results when tissue biopsies are used as the source of lesion material for diagnosis of CL.


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  1. Alvar J, Vélez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, den Boer M, WHO Leishmaniasis Control Team; , 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS One 7: e35671. [Google Scholar]
  2. Karunaweera ND, Rajapaksa US, , 2009. Is leishmaniasis in Sri Lanka benign and be ignored? J Vector Borne Dis 46: 1317. [Google Scholar]
  3. Siriwardana H, Chandrawansa PH, Sirimanna G, Karunaweera ND, , 2012. Leishmaniasis in Sri Lanka: a decade old story. Sri Lanka J Infect Dis 2: 212. [Google Scholar]
  4. de Vries HJC, Reedijk SH, Schallig HDFH, , 2015. Cutaneous leishmaniasis: recent developments in diagnosis and management. Am J Clin Dermatol 16: 99109. [Google Scholar]
  5. Aronson N, Herwaldt BL, Libman M, Pearson R, Lopez-Velez R, Weina P, Carvalho EM, Ephros M, Jeronimo S, Magill A, , 2016. Diagnosis and treatment of leishmaniasis: clinical practice guidelines by the Infectious Diseases Society of America (IDSA) and the American Society of Tropical Medicine and Hygiene (ASTMH). Clin Infect Dis 63: 15391557. [Google Scholar]
  6. Ranawaka RR, Abeygunasekara PH, Weerakoon HS, , 2012. Correlation of clinical, parasitological and histopathological diagnosis of cutaneous leishmaniasis in an endemic region in Sri Lanka. Ceylon Med J 57: 149152. [Google Scholar]
  7. Center for Disease Control and Prevention, 2011. Practical Guide for Laboratory Diagnosis of Leishmaniasis. Available at: https://www.cdc.gov/parasites/leishmaniasis/health_professionals/index.html#dx. Accessed March 15, 2013.
  8. World Health Organization, 2010. Control of the leishmaniases. World Health Organ Tech Rep Ser 949: 2226. Available at: http://www.who.int/leishmaniasis/resources/technical_report_series/en/. Accessed June 30, 2017. [Google Scholar]
  9. Kaul N, Gupta V, Bhardwaj S, Dogra D, Dogra N, , 2016. A new focus of cutaneous leishmaniasis in Jammu division of Jammu and Kashmir State, India. Indian J Dermatol Venereol Leprol 82: 145150. [Google Scholar]
  10. Al-hucheimi SN, Sultan BA, Al-Dhalimi MA, , 2009. A comparative study of the diagnosis of Old World cutaneous leishmaniasis in Iraq by polymerase chain reaction and microbiologic and histopathologic methods. Int J Dermatol 48: 404408. [Google Scholar]
  11. ul Bari A, ber Rahman S, , 2006. Correlation of clinical, histopathological, and microbiological findings in 60 cases of cutaneous leishmaniasis. Indian J Dermatol Venereol Leprol 72: 2832. [Google Scholar]
  12. Reed SG, , 1996. Diagnosis of leishmaniasis. Clin Dermatol 14: 471478. [Google Scholar]
  13. Ramírez JR, Agudelo S, Muskus C, Alzate JF, Berberich C, Barker D, Velez ID, , 2000. Diagnosis of cutaneous leishmaniasis in Colombia: the sampling site within lesions influences the sensitivity of parasitologic diagnosis. J Clin Microbiol 38: 37683773. [Google Scholar]
  14. Bahamdan KA, Khan AR, Tallab TM, Mourad MM, , 1996. Value of touch preparations (imprints) for diagnosis of cutaneous leishmaniasis. Int J Dermatol 35: 558560. [Google Scholar]
  15. Sousa AQ, Pompeu MML, Frutuoso MS, Lima JWO, Tinel JMBM, Pearson RD, , 2014. Short report: press imprint smear: a rapid, simple, and cheap method for the diagnosis of cutaneous leishmaniasis caused by Leishmania (Viannia) braziliensis. Am J Trop Med Hyg 91: 905907. [Google Scholar]
  16. Kirstein OD, Abbasi I, Horwitz BZ, Skrip L, Hailu A, Jaffe C, Li LL, Prow TW, Warburg A, , 2017. Minimally invasive microbiopsies: a novel sampling method for identifying asymptomatic, potentially infectious carriers of Leishmania donovani. Int J Parasitol 47: 609616. [Google Scholar]
  17. de Mello CX, Madeira M de F, , 2015. Skin scraping is the most accessible technique for the parasitological diagnosis of American tegumentary leishmaniasis. Am J Trop Med Hyg 92: 1082. [Google Scholar]
  18. Berger RS, Perez-Figaredo RA, Spielvogel RL, , 1987. Leishmaniasis: the touch preparation as a rapid means of diagnosis. J Am Acad Dermatol 16: 10961105. [Google Scholar]

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  • Received : 26 Sep 2017
  • Accepted : 10 Dec 2017
  • Published online : 15 Jan 2018

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