1921
Volume 99, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

is an emerging zoonotic pathogen present in the United States, South America, and Europe. The molecular detection of frequently relies on polymerase chain reaction (PCR) assays that target the genus coupled with DNA sequencing for species determination. However, the presence of other spp. in the sample being tested may result in false-negative results for , especially when Sanger sequencing is used. We developed a sensitive and specific quantitative PCR platform for by targeting the intergenic transcribed spacer, , and genes, which are recommended for subtyping characterization. This PCR platform achieved the limit of detection between five and 10 genomic equivalents per reaction and did not amplify DNA from other species or selected hosts. This PCR platform is a fast and cost-effective option to be used in epidemiological evaluations of reservoirs and vectors and in detecting and quantifying infection in humans.

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Supplemental file

  • Received : 23 Sep 2017
  • Accepted : 24 Jun 2018
  • Published online : 06 Aug 2018

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