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image of Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

The aim of this study was to develop a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method for specific diagnosis of . With incubation at 37°C, the gene of was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solution, the amplified RPA product can be visualized on a LF strip. The RPA assay exhibited high sensitivity with limits of detection down to 10 parasites/μL of . Nonetheless, it was demonstrated that all ( = 41) and other sp. ( = 25) were positive while negative samples ( = 8) were negative. Therefore, a combination of RPA and LF strip detection is a highly promising approach with the potential to be suitable for use in resource-limited settings.

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/content/journals/10.4269/ajtmh.17-0738
2017-12-18
2018-01-16
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.17-0738
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  • Published online : 18 Dec 2017
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