1921
Volume 98, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract.

Pyrethroid resistance has been detected in (Hemiptera: Reduviidae), which was atributed to target site insensitivity and increased oxidative metabolism of the insecticide by cytochrome P450s. Nicotinamide adenine dinucleotide phosphate (NADPH) cytochrome P450 reductase (CPR) plays an essential role in transferring electrons from NADPH to the P450-substrate complex. In this study, the full length cDNA of was isolated and gene expression was determined by quantitative polymerase chain reaction. The open reading frame is 2,046 bp long, encoding a protein of 682 amino acids. Amino acid sequence analysis indicates that the CPR and the putative and CPRs present conserved ligand-binding domains. Congruent with a previous study of our laboratory, in which the expression of three cytochrome genes (, , and genes) was induced by deltamethrin, the levels of mRNA were upregulated in the fat body of fifth instar nymphs after topical application of deltamethrin. Besides, as it was observed in the gene, it was detected overexpression of the gene in the most resistant strain of included in the study. These results suggest that CPR plays an essential role in P450-mediated resistance of to insecticides.

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/content/journals/10.4269/ajtmh.17-0404
2018-03-07
2018-09-21
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  • Received : 23 May 2017
  • Accepted : 02 Dec 2017

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