1921
Volume 97, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

The lentil lectin glycoprotein enzyme-linked immunoelectrotransfer blot (LLGP EITB, reported sensitivity 99% and specificity 100%) is used as a serologic marker of exposure to in pigs. However, only a limited number of parasites have been evaluated for cross reactivity. Pigs may host other related cestode infections, including which have not been formally evaluated for cross-reactions. We investigated a corral in Tumbes, Peru, a region where a cysticercosis elimination demonstration project was completed in 2012. In this corral, 14/19 (73.7%) 6–8-week-old piglets were reactive to GP50 on LLGP EITB, and all had circulating sp. antigens. From eight necropsied piglets; four were infected with metacestodes whereas none had evidence of infection. Two resident dogs were subsequently confirmed to have taeniasis. These results suggest GP50 cross-reactivity in infected pigs, although controlled experimental infection is needed to confirm this hypothesis.

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References

  1. Gonzalez AE, Gilman R, Garcia HH, McDonald J, Kacena K, Tsang VC, Pilcher JB, Suarez F, Gavidia C, Miranda E, 1994. Use of sentinel pigs to monitor environmental Taenia solium contamination. The Cysticercosis Working Group in Peru (CWG). Am J Trop Med Hyg 51: 847850.
    [Google Scholar]
  2. García HH et al., Cysticercosis Working Group in Perú, 2003. Hyperendemic human and porcine Taenia solium infection in Perú. Am J Trop Med Hyg 68: 268275.
    [Google Scholar]
  3. O’Neal SE, Moyano LM, Ayvar V, Rodriguez S, Gavidia C, Wilkins PP, Gilman RH, Garcia HH, Gonzalez AE; Cysticercosis Working Group in Peru, 2014. Ring-screening to control endemic transmission of Taenia solium. PLoS Negl Trop Dis 8: e3125.
    [Google Scholar]
  4. Gavidia CM, Verastegui MR, Garcia HH, Lopez-Urbina T, Tsang VC, Pan W, Gilman RH, Gonzalez AE; Cysticercosis Working Group in Peru, 2013. Relationship between serum antibodies and Taenia solium larvae burden in pigs raised in field conditions. PLoS Negl Trop Dis 7: e2192.
    [Google Scholar]
  5. Mohan VR, Tharmalingam J, Muliyil J, Oommen A, Dorny P, Vercruysse J, Vedantam R, 2013. Prevalence of porcine cysticercosis in Vellore, South India. Trans R Soc Trop Med Hyg 107: 6264.
    [Google Scholar]
  6. Sikasunge CS, Phiri IK, Phiri AM, Siziya S, Dorny P, Willingham AL 3rd, 2008. Prevalence of Taenia solium porcine cysticercosis in the eastern, southern and western provinces of Zambia. Vet J 176: 240244.
    [Google Scholar]
  7. Tsang VC, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159: 5059.
    [Google Scholar]
  8. Tsang VC, Pilcher JA, Zhou W, Boyer AE, Kamango-Sollo EI, Rhoads ML, Murrell KD, Schantz PM, Gilman RH, 1991. Efficacy of the immunoblot assay for cysticercosis in pigs and modulated expression of distinct IgM/IgG activities to Taenia solium antigens in experimental infections. Vet Immunol Immunopathol 29: 6978.
    [Google Scholar]
  9. Garcia HH et al., 2016. Elimination of Taenia solium transmission in northern Peru. N Engl J Med 374: 23352344.
    [Google Scholar]
  10. Brandt JR, Geerts S, De Deken R, Kumar V, Ceulemans F, Brijs L, Falla N, 1992. A monoclonal antibody-based ELISA for the detection of circulating excretory-secretory antigens in Taenia saginata cysticercosis. Int J Parasitol 22: 471477.
    [Google Scholar]
  11. Nguekam A, Zoli AP, Vondou L, Pouedet SM, Assana E, Dorny P, Brandt J, Losson B, Geerts S, 2003. Kinetics of circulating antigens in pigs experimentally infected with Taenia solium eggs. Vet Parasitol 111: 323332.
    [Google Scholar]
  12. Guezala MC, Rodriguez S, Zamora H, Garcia HH, Gonzalez AE, Tembo A, Allan JC, Craig PS, 2013. Development of a species-specific coproantigen ELISA for human Taenia solium taeniasis. Am J Trop Med Hyg 81: 433437.
    [Google Scholar]
  13. Gemmell MA, 1973. Surveillance of Echinococcus granulosus in dogs with arecoline hydrobromide. Bull World Health Organ 48: 649652.
    [Google Scholar]
  14. Liu GH, Lin RQ, Li MW, Liu W, Liu Y, Yuan ZG, Song HQ, Zhao GH, Zhang KX, Zhu XQ, 2011. The complete mitochondrial genomes of three cestode species of Taenia infecting animals and humans. Mol Biol Rep 38: 22492256.
    [Google Scholar]
  15. Bowles J, McManus DP, 1994. Genetic characterization of the Asian Taenia, a newly described taeniid cestode of humans. Am J Trop Med Hyg 50: 3344.
    [Google Scholar]
  16. Zhan G, Chen J, Yang Y, Liu N, Jiang W, Gu S, Wang X, Wang Z, 2014. Utility of DNA barcoding in distinguishing species of the family Taeniidae. J Parasitol 100: 542546.
    [Google Scholar]
  17. Dorny P, Phiri IK, Vercruysse J, Gabriel S, Willingham AL 3rd, Brandt J, Victor B, Speybroeck N, Berkvens D, 2004. A Bayesian approach for estimating values for prevalence and diagnostic test characteristics of porcine cysticercosis. Int J Parasitol 34: 569576.
    [Google Scholar]
  18. Cheng RWK, Ko R, 1991. Cross-reactions between crude antigens of larval Taenia solium (Cysticercus cellulosae) and other helminths of pigs. Vet Parasitol 39: 161170.
    [Google Scholar]
  19. Nguyen MT, Gabriël S, Abatih EN, Dorny P, 2016. A systematic review on the global occurrence of Taenia hydatigena in pigs and cattle. Vet Parasitol 226: 97103.
    [Google Scholar]
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  • Received : 12 May 2017
  • Accepted : 08 Aug 2017
  • Published online : 02 Oct 2017
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