1921
image of Molecular Detection and Characterization of Wolbachia pipientis from Culex quinquefasciatus Collected from Lahore, Pakistan
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

The gram-negative, pleomorphic endosymbiont is known to infect a large number of insects and other arthropods naturally. This bacterium modifies the host biology, mainly causing reproductive alterations including feminization, death of male, parthenogenesis, and importantly cytoplasmic incompatibility. -induced cytoplasmic incompatibility results in nonviable offspring and vector population suppression. In addition, this bacterium rapidly spreads and propagates within the host population. This study is the first report on detection and characterization from collected from Lahore, Pakistan. For this purpose, mosquito adults were collected from different localities of Lahore and identified at the species level. A total of 145 pairs of ovaries were individually subjected to DNA isolation, and polymerase chain reaction amplification of three (, 16S rRNA, and ) genes were investigated. In all, 128 females were found positive, representing 82.3% infection rate. The phylogenetic analysis indicated that the detected endosymbiont had 100% homology with Pip strain and supergroup B. The detection of the local strain of (Pip) will be useful in investigating its potential for the control of dengue vector () and reducing dengue transmission in Pakistan.

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/content/journals/10.4269/ajtmh.17-0329
2017-10-23
2017-11-22
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.17-0329
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  • Published online : 23 Oct 2017
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