Volume 97, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We analyzed the accuracy of Chagas Quick Test, a rapid diagnostic test, for the diagnosis of chronic Chagas disease through a retrospective study on a cohort of 669 patients consecutively examined at a single reference center in Italy, during a 7-year period. We observed high concordance with serological reference standard but low accuracy for screening purposes (sensitivity/specificity: 82.8%/98.7%) at least in our nonendemic context.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...



  1. Rassi A Jr, Rassi A, Marcondes de Rezende J, 2012. American trypanosomiasis (Chagas disease). Infect Dis Clin North Am 26: 275291.[Crossref]
    [Google Scholar]
  2. World Health Organization, 2007. WHO Consultation on International Biological Reference. Preparation for Chagas Diagnostic Tests. Available at: http://www.who.int/bloodproducts/ref_materials/WHO_Report_1st_Chagas_BRP_consultation_7-2007_final.pdf. Accessed March 13, 2017.
  3. World Health Organization, 2002. WHO Expert Committee on the Control of Chagas Disease (2000: Brasilia). Control of Chagas Disease: Second Report of the WHO Expert Committee. Available at: http://apps.who.int/iris/bitstream/10665/42443/1/WHO_TRS_905.pdf. Accessed March 13, 2017.
  4. Brasil PE, De Castro L, Hasslocher Moreno AM, Sangenis LH, Braga JU, 2010. ELISA versus PCR for diagnosis of chronic Chagas disease: systematic review and meta-analysis. BMC Infect Dis 10: 337.[Crossref]
    [Google Scholar]
  5. Sánchez-Camargo CL et al., 2014. Comparative evaluation of 11 commercialized rapid diagnostic tests for detecting Trypanosoma cruzi antibodies in serum banks in areas of endemicity and non endemicity. J Clin Microbiol 52: 25062512.[Crossref]
    [Google Scholar]
  6. Chappuis F, Mauris A, Holst M, Albajar-Viñas P, Jannin J, Luquetti AO, Jackson Y, 2010. Validation of a rapid immunochromatographic assay for diagnosis of Trypanosoma cruzi infection among Latin American migrants in Geneva, Switzerland. J Clin Microbiol 48: 29482952.[Crossref]
    [Google Scholar]
  7. Flores-Chavez M, Cruz I, Nieto J, Gárate T, Navarro M, Pérez-Ayala A, López-Vélez R, Cañavate C, 2012. Sensitivity and specificity of an operon immunochromatographic test in serum and whole blood samples for the diagnosis of Trypanosoma cruzi infection in Spain, an area of non endemicity. Clin Vaccine Immunol 19: 13531359.[Crossref]
    [Google Scholar]
  8. Reithinger R, Grijalva MJ, Chiriboga RF, de Noya BA, Torres JR, Pavia-Ruz N, Manrique-Saide P, Cardinal MV, Gürtler RE, 2010. Rapid detection of Trypanosoma cruzi in human serum by use of an immunochromatographic dipstick test. J Clin Microbiol 48: 30033007.[Crossref]
    [Google Scholar]
  9. Cypress Diagnostics, 2013. Chagas Quick Test leaflet. Available at: http://www.genius-diagnostics.al/pdf/Chagas.pdf. Accessed March 13, 2017.
  10. Carley S, Dosman S, Jones SR, Harrison M, 2005. Simple nomograms to calculate sample size in diagnostic studies. Emerg Med J 22: 180181.[Crossref]
    [Google Scholar]
  11. Leeflang MM, 2014. Systematic reviews and meta-analyses of diagnostic test accuracy. Clin Microbiol Infect 20: 105113.[Crossref]
    [Google Scholar]
  12. López-Chejade P, Roca C, Posada E, Pinazo MJ, Gascon J, Portús M, 2010. Utility of an immunochromatographic test for Chagas disease screening in primary healthcare. Enferm Infecc Microbiol Clin 28: 169171.[Crossref]
    [Google Scholar]
  13. Navarro M, Perez-Ayala A, Guionnet A, Perez-Molina JA, Navaza B, Estevez L, Norman F, Flores-Chavez M, Lopez-Velez R, 2011. Targeted screening and health education for Chagas disease tailored to at-risk migrants in Spain, 2007 to 2010. Euro Surveill 16: 19973.[Crossref]
    [Google Scholar]

Data & Media loading...

  • Received : 14 Mar 2017
  • Accepted : 05 Jul 2017
  • Published online : 14 Aug 2017
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error