Volume 97, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



In Senegal, antimalarial drugs used in treatment and prevention of malaria are one of the main reasons for the current success in controlling malaria. However, the successful control of malaria is highly dependent on continued effectiveness of these drugs which may be compromised by the spread of drug resistance. Therefore, surveillance of drug resistance in the malaria parasites is essential. The objective of this pilot study was to test the feasibility of routinely sampled malaria rapid diagnostic tests (RDTs) at a national scale to assess the temporal changes in the molecular profiles of antimalarial drug resistance markers of parasites. Overall, 9,549 positive malaria RDTs were collected from 14 health facilities across the country. A limited random set of RDTs were analyzed regarding gene polymorphisms at codon 72–76. Overall, a high but varied prevalence (> 50%) of the wild-type CVMNK haplotype was observed including a higher CVMNK prevalence in the northern part (75%) compared with the southern part of the country (59%). With caution, the study provides a proof of concept that reuse of discarded positive RDTs can be applied in large-scale surveillance of antimalarial drug resistance.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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  1. Gaye O, Soumare M, Sambou B, Faye O, Dieng Y, Diouf M, Bah IB, Dieng T, Ndir O, Diallo S, 1999. Heterogeneity of chloroquine resistant malaria in Senegal. Bull Soc Pathol Exot 92: 149152.
    [Google Scholar]
  2. Botella DMJ, Valls FJM, Martinez PML, Espacio CA, 1991. Plasmodium falciparum resistant to sulfadoxine/pyrimethamine in Senegal. Med Interna 8: 7981.
    [Google Scholar]
  3. Sokhna CS, Trape JF, Robert V, 2001. Gametocytaemia in Senegalese children with uncomplicated falciparum malaria treated with chloroquine, amodiaquine or sulfadoxine plus pyrimethamine. Parasite 8: 243250.[Crossref]
    [Google Scholar]
  4. Brasseur P, Diallo S, Guiguemde R, Guiyedi V, Kombila M, Ringwald P, Olliaro P, 1999. Amodiaquine remains effective for treating uncomplicated malaria in west and central Africa. Trans R Soc Trop Med Hyg 93: 645650.[Crossref]
    [Google Scholar]
  5. World Health Organization, 2015. World Malaria Report 2015. Available at: http://www.who.int/malaria/publications/world-malaria-report-2015/report/en/. Accessed November 1, 2017.
  6. World Health Organization, 2004/2006. The role of laboratory diagnosis to support malaria disease management: focus on the use of rapid diagnostic tests in areas of high transmission. Report of a WHO Technical Consultation, 25–26 Oct 2004. Geneva, Switzerland: WHO.
  7. World Health Organization, 2006. The Use of Malaria Rapid Diagnostic Tests, 2nd edition. Geneva, Switzerland: WHO. Available at: http://www.who.int/malaria/publications/use-malaria-rdts/en/.
  8. Roll Back Malaria Partnership, 2010. Roll Back Malaria. Progress and Impact Series: Focus on Senegal. Available at: http://www.rbm.who.int/ProgressImpactSeries/report4.
  9. Cnops L, Boderie M, Gillet P, Van Esbroeck M, Jacobs J, 2011. Rapid diagnostic testsas a source of DNA for Plasmodium species-specific real-time PCR. Malar J 10: 67.[Crossref]
    [Google Scholar]
  10. Thiam S et al., 2011. Major reduction in anti-malarial drug consumption in Senegal after nation-wide introduction of malaria rapid diagnostic tests. PLoS One 6: e18419.[Crossref]
    [Google Scholar]
  11. Ishengoma DS, Lwitiho S, Madebe RA, Nyagonde N, Persson O, Vestergaard LS, Bygbjerg IC, Lemnge MM, Alifrangis M, 2011. Using rapid diagnostic tests as source of malaria parasite DNA for molecular analyses in the era of declining malaria prevalence. Malar J 10: 6.[Crossref]
    [Google Scholar]
  12. Available at: http://www.wwarn.org/sites/default/files/attachments/procedures/MOL06_RDTs For DNA Extraction.pdf. Accessed April 2017.
  13. Wooden J, Kyes S, Sibley CH, 1993. PCR and strain identification in Plasmodium falciparum . Parasitol Today 9: 303305.[Crossref]
    [Google Scholar]
  14. Alifrangis M, Enosse S, Pearce R, Drakeley C, Roper C, Khalil IF, Nkya WM, Rønn AM, Theander TG, Bygbjerg IC, 2005. A simple, high-throughput method to detect Plasmodium falciparum single nucleotide polymorphisms in the dihydrofolatereductase, dihydropteroate synthase, and P. falciparum chloroquine resistance transporter genes using polymerase chain reaction- and enzyme-linked immunosorbent assay-based technology. Am J Trop Med Hyg 72: 155162.
    [Google Scholar]
  15. Ndiaye M et al., 2012. Assessment of the molecular marker of Plasmodium falciparum chloroquine resistance (Pfcrt) in Senegal after several years of chloroquine withdrawal. Am J Trop Med Hyg 87: 640645.[Crossref]
    [Google Scholar]
  16. Naidoo I, Roper C, 2013. Mapping ‘partially resistant’, ‘fully resistant’, and ‘super resistant’ malaria. Trends Parasitol 29: 505515.[Crossref]
    [Google Scholar]
  17. Ariey F et al., 2014. A molecular marker of artemisinin-resistant Plasmodium falciparum malaria. Nature 505: 5055.[Crossref]
    [Google Scholar]
  18. Roper C, Alifrangis M, Ariey F, Talisuna A, Menard D, Mercereau-Puijalon O, Ringwald P, 2014. Molecular surveillance for artemisinin resistance in Africa. Lancet Infect Dis 14: 668670.[Crossref]
    [Google Scholar]

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  • Received : 10 Jan 2017
  • Accepted : 16 May 2017
  • Published online : 28 Aug 2017
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