1921
Volume 97, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

RNA interference caused by exogenous double-stranded RNA (dsRNA) is used to downregulate crucial genes to control insects. The reproductive success of all oviparous species depends on vitellogenin (Vg) biosynthesis and its accumulation in the developing oocytes. Adult females of were independently injected with two dsRNAs ( dsRNA or dsRNA) or nuclease-free water (control) 24 hours before feeding, and a group of adult females not injected was also analyzed (control). and messenger RNAs silencing was verified by reverse transcription quantitative polymerase chain reaction. The transcript levels of the and genes were significantly reduced after dsRNA treatment in fat body and ovary of in relation to those detected in individuals injected with nuclease-free water and not injected (controls). Moreover, the present study demonstrated that the silencing of the or genes inhibits oviposition in the Chagas disease vector . These findings may have important implications for the development of novel vector control strategies.

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2017-08-02
2018-11-17
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  • Received : 29 Dec 2016
  • Accepted : 15 Apr 2017

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