1921
Volume 96, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

The utility of applying infected to Flinders Technology Associates (FTA) cards for storage, transport, and detection of dengue, Zika, and Barmah Forest viruses was assessed in laboratory-based experiments. The mosquitoes had been removed from Gravid Traps maintained under conditions of high temperature and humidity. RNA of all viruses could be detected in infected mosquitoes on FTA cards either individually or in pools with uninfected mosquitoes, and stored for up to 28 days. Importantly, there was only a minimal decrease in RNA levels in mosquitoes between days 0 and 28, indicating that viral RNA was relatively stable on the cards. FTA cards thus provide a mechanism for storing potentially infected mosquitoes collected in the field and transporting them to a central diagnostic facility for virus detection.

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2017-05-03
2018-10-19
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References

  1. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, Drake JM, Brownstein JS, Hoen AG, Sankoh O, Myers MF, George DB, Jaenisch T, Wint GR, Simmons CP, Scott TW, Farrar JJ, Hay SI, , 2013. The global distribution and burden of dengue. Nature 496: 504507.[Crossref]
  2. Petersen LR, Jamieson DJ, Powers AM, Honein MA, , 2016. Zika virus. N Engl J Med 374: 15521563.[Crossref]
  3. Weaver SC, Lecuit M, , 2015. Chikungunya virus and the global spread of a mosquito-borne disease. N Engl J Med 372: 12311239.[Crossref]
  4. Gama RA, Silva EM, Silva IM, Resende MC, Eiras AE, , 2007. Evaluation of the sticky MosquiTRAP for detecting Aedes (Stegomyia) aegypti (L.) (Diptera: Culicidae) during the dry season in Belo Horizonte, Minas Gerais, Brazil. Neotrop Entomol 36: 294302.[Crossref]
  5. Krockel U, Rose A, Eiras AE, Geier M, , 2006. New tools for surveillance of adult yellow fever mosquitoes: comparison of trap catches with human landing rates in an urban environment. J Am Mosq Control Assoc 22: 229238.[Crossref]
  6. Eiras AE, Buhagiar TS, Ritchie SA, , 2014. Development of the Gravid Aedes Trap for the capture of adult female container-exploiting mosquitoes (Diptera: Culicidae). J Med Entomol 51: 200209.[Crossref]
  7. Ritchie SA, Long S, Smith G, Pyke A, Knox TB, , 2004. Entomological investigations in a focus of dengue transmission in Cairns, Queensland, Australia, by using the sticky ovitraps. J Med Entomol 41: 14.[Crossref]
  8. Vilela AP, Figueiredo LB, dos Santos JR, Eiras AE, Bonjardim CA, Ferreira PC, Kroon EG, , 2010. Dengue virus 3 genotype I in Aedes aegypti mosquitoes and eggs, Brazil, 2005–2006. Emerg Infect Dis 16: 989992.[Crossref]
  9. Ritchie SA, Buhagiar TS, Townsend M, Hoffmann A, Van Den Hurk AF, McMahon JL, Eiras AE, , 2014. Field validation of the Gravid Aedes Trap (GAT) for collection of Aedes aegypti (Diptera: Culicidae). J Med Entomol 51: 210219.[Crossref]
  10. Abdelwhab EM, Luschow D, Harder TC, Hafez HM, , 2011. The use of FTA® filter papers for diagnosis of avian influenza virus. J Virol Methods 174: 120122.[Crossref]
  11. Barth H, Morel A, Mougin C, Averous G, Legrain M, Fender M, Risch S, Fafi-Kremer S, Velten M, Oudet P, Baldauf JJ, Stoll-Keller F, , 2016. Long-term storage and safe retrieval of human papillomavirus DNA using FTA elute cards. J Virol Methods 229: 6065.[Crossref]
  12. Hall-Mendelin S, Ritchie SA, Johansen CA, Zborowski P, Cortis G, Dandridge S, Hall RA, van den Hurk AF, , 2010. Exploiting mosquito sugar feeding to detect mosquito-borne pathogens. Proc Natl Acad Sci USA 107: 1125511259.[Crossref]
  13. Pyke AT, Daly MT, Cameron JN, Moore PR, Taylor CT, Hewitson GR, Humphreys JL, Gair R, , 2014. Imported Zika virus infection from the Cook Islands into Australia, 2014. PLoS Curr 6. doi:10.1371/currents.outbreaks.849adc0ad16beec4536695281707f785.
  14. van den Hurk AF, Hall-Mendelin S, Townsend M, Kurucz N, Edwards J, Ehlers G, Rodwell C, Moore FA, McMahon JL, Northill JA, Simmons RJ, Cortis G, Melville L, Whelan PI, Ritchie SA, , 2014. Applications of a sugar-based surveillance system to track arboviruses in wild mosquito populations. Vector Borne Zoonotic Dis 14: 6673.[Crossref]
  15. Warrilow D, Northill JA, Pyke A, Smith GA, , 2002. Single rapid TaqMan fluorogenic probe based PCR assay that detects all four dengue serotypes. J Med Virol 66: 524528.[Crossref]
  16. Bangs MJ, Tan R, Listiyaningsih E, Kay BH, Porter KR, , 2001. Detection of dengue viral RNA in Aedes aegypti (Diptera: Culicidae) exposed to sticky lures using reverse-transcriptase polymerase chain reaction. J Med Entomol 38: 720724.[Crossref]
  17. Johansen CA, Hall RA, van den Hurk AF, Ritchie SA, Mackenzie JS, , 2002. Detection and stability of Japanese encephalitis virus RNA and virus viability in dead infected mosquitoes under different storage conditions. Am J Trop Med Hyg 67: 656661.
  18. Turell MJ, Spring AR, Miller MK, Cannon CE, , 2002. Effect of holding conditions on the detection of West Nile viral RNA by reverse transcriptase-polymerase chain reaction from mosquito (Diptera: Culicidae) pools. J Med Entomol 39: 13.[Crossref]
  19. Hill LA, Davis JB, Hapgood G, Whelan PI, Smith GA, Ritchie SA, Cooper RD, van den Hurk AF, , 2008. Rapid identification of Aedes albopictus, Aedes scutellaris, and Aedes aegypti life stages using real-time polymerase chain reaction assays. Am J Trop Med Hyg 79: 866875.
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  • Received : 15 Dec 2016
  • Accepted : 14 Jan 2017

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