1921
Volume 97, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract.

Scabies is a human skin disease due to the burrowing ectoparasite var. resulting in intense itching and inflammation and manifesting as a skin allergy. Because of insufficient mite material and lack of in vitro propagation system for antigen preparation, scabies is a challenging disease to develop serological diagnostics. For allergen characterization, full-length tropomyosin (Sar s 10) was cloned, expressed in pET-15b, and assessed for reactivity with IgE antibodies from human sera. IgE binding was observed to Sar s 10 with sera collected from subjects with ordinary scabies, house dust mite (HDM)-positive and naive subjects and a diagnostic sensitivity of < 30% was observed. paramyosin (Sar s 11) was cloned, and expressed in pET-28a in three overlapping fragments designated Sspara1, Sspara2, and Sspara3. IgE and IgG binding was observed to Sspara2 and Sspara3 antigens with sera collected from ordinary scabies, and HDM-positive subjects, but no binding was observed with sera collected from naive subjects. Sspara2 displayed excellent diagnostic potential with 98% sensitivity and 90% specificity observed for IgE binding and 70% sensitivity for IgG. In contrast, the diagnostic sensitivity of Sspara3 was 84% for IgE binding and 40% for IgG binding. In combination, Sspara2 and Sspara3 provided an IgE sensitivity of 94%. This study shows that IgE binding to Sspara2 and Sspara3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice. The developed enzyme-linked immunosorbent assay helps direct future development of a specific diagnostic tool for scabies.

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2017-09-07
2018-11-20
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References

  1. Mellanby K, , 1944. The development of symptoms, parasitic infections and immunity in human scabies. Parasitol 35: 197206.
  2. Orkin M, Maibach H, , 1985. Cutaneous Infestation and Insect Bites. New York, NY: Marcel Deeker.
  3. Pence DB, Ueckermann E, , 2002. Sarcoptic mange in wildlife. Rev Sci Tech 21: 385398.
  4. Walton SF, Currie BJ, , 2007. Problems in diagnosing scabies, a global disease in human and animal populations. Americ Soci for Microbiol 20: 268279.
  5. Hengge UR, Currie BJ, Jager G, Lupi O, Schwartz RA, , 2006. Scabies: a ubiquitous neglected skin disease. Lancet Infect Dis 6: 769779.
  6. Payne L, Fitchett JR, , 2010. Bringing neglected tropical diseases into the spotlight. Trends Parasitol 26: 421423.
  7. Andrews RM, McCarthy J, Carapetis JR, Currie BJ, , 2009. Skin disorders, including pyoderma, scabies, and tinea infections. Pediatr Clin North Am 56: 14211440.
  8. Walton SF, Currie BJ, Kemp DJ, , 1997. A DNA fingerprinting system for the ectoparasite Sarcoptes scabiei. Mol Biochem Parasitol 85: 187196.
  9. Currie B, Huffam S, O’Brien D, Walton S, , 1997. Ivermectin for scabies. Lancet 350: 1551.
  10. Roberts LJ, Huffam SE, Walton SF, , 2005. Crusted scabies: clinical and immunological findings in seventy-eight patients and a review of the literature. J Infect 50: 375381.
  11. Granholm JM, Olazewski J, ,2005. Scabies Prevention and Control Manual. Fenton, MI: Michigan Department of Community Health.
  12. Jayaraj RB, Viberg HL, Pizzuto S, Holt D, Rolland JM, O’hehir JRE, Currie BJ, Walton SF, , 2011. A diagnostic test for scabies: IgE specificity for recombinant allergen of Sarcoptes scabiei. Diagn Microbiol Infect Dis 71: 403407.
  13. Walter B, Heukelbach J, Fengler G, Worth C, Hengge U, Feldmeier H, , 2011. Comparison of dermoscopy, skin scraping and the adhesive tape test for the diagnosis of scabies in a resource poor setting. Arch Dermatol 147: 468473.
  14. Fukuyama S, Nishimura T, Yotsumoto H, Gushi A, Tsuji M, Kanekura T, Matsuyama T, , 2010. Diagnostic usefulness of a nested polymerase chain reaction assay for detecting Sarcoptes scabiei DNA in skin scrapings from clinically suspected scabies. Br J Dermatol 163: 875899.
  15. Guldbakke KK, Khachemoune A, , 2006. Crusted scabies: a clinical review. J Drugs Dermatol 5: 221227.
  16. Bornstein S, Thebo P, Zakrisson G, , 1996. Evaluation of an enzyme-linked immunosorbant assay (ELISA) for the serological diagnosis of canine sarcoptic mange. Vet Derm 7: 2128.
  17. Bornstein S, Wallgren P, , 1997. Serodiagnosis of sarcoptic mange in pigs. Vet Rec 141: 812.
  18. Lowenstein M, Kahlbacher H, Peschke R, , 2004. On the substantial variation in serological responses in pigs to Sarcoptes scabiei var. suis using different commercially available indirect enzyme-linked immunosorbent assays. Parasitol Res 94: 2430.
  19. Casais R, Miguel P, Ana B, Paloma S, Francisco P, José MA, , 2007. Identification and heterologous expression of a Sarcoptes scabiei cDNA encoding a structural antigen with immunodiagnostic potential. Vet Res 38: 435450.
  20. Fischer K, Holt DC, Harumal P, Currie BJ, Walton SF, Kemp DJ, , 2003. Generation and characterization of cDNA clones from Sarcoptes scabiei var. hominis for an expressed sequence tag library: identification of homologues of house dust mite allergens. Am J Trop Med Hyg 68: 6164.
  21. Harumal P, Morgan M, Walton SF, Holt DC, Rode J, Arlian LG, Currie BJ, Kemp DJ, , 2003. Identification of a homologe of a house dust mite allergen in a cDNA library from Sarcoptes scabiei var. hominis and evaluation of its vaccine potential in a rabbit/S. scabiei var. canis model. Am J Trop Med Hyg 68: 5460.
  22. Holt DC, Fischer K, Allen GE, Wilson D, Wilson P, Slade R, Currie BJ, Walton SF, Kemp DJ, , 2003. Mechanisms for a novel immune evasion strategy in the scabies mite Sarcoptes scabiei: a multigene family of inactivated serine proteases. J Invest Dermatol 121: 14191424.
  23. Ljunggren EL, Nilsson D, Mattsson JG, , 2003. Expressed sequence tag analysis of Sarcoptes scabiei. Parasitol 127: 139145.
  24. Dougall A, Holt DC, Fischer K, Currie BJ, Kemp DJ, Walton SF, , 2005. Identification and characterization of Sarcoptes scabiei and Dermatophagoide spteronyssinus glutathione S-transferases: implication as a potential major allergen in crusted scabies. Am J Trop Med Hyg 73: 977984.
  25. Walton SF, Pizzutto S, Slender A, Viberg L, Holt D, Hales BJ, Kemp DJ, Currie BJ, Rolland JM, O’Hehir R, , 2010. Increased allergic immune response to Sarcoptes scabiei antigens in crusted versus ordinary scabies. Clin Vaccine Immunol 17: 14281438.
  26. Rampton M, Walton SF, Holt DC, Pasay C, Kelly A, Currie BJ, McCarthy JS, Mounsey KE, , 2013. Antibody responses to Sarcoptes scabiei apolipoprotein in a porcine model: relevance to immunodiagnosis of recent infection. PLoS One 8: e65354.
  27. Zheng Y, He R, He M, Gu X, Wang T, Lai W, Peng X, Yang G, , 2016. Characterization of Sarcoptes scabiei cofilin gene and assessment of recombinant cofilin protein as an antigen in indirect ELISA for diagnosis. BMC Infect Dis 16: 21.
  28. Fernandes J, Reshef A, Patton L, Ayuso R, Reese G, , 2003. Immunoglobulin E antibody reactivity to the major shrimp allergen, tropomyosin, in unexposed Orthodox Jews. Clin Exp Allergy 33: 956961.
  29. Yi FC, Cheong N, Shek PC, Wang DY, Chua KY, Lee BW, , 2002. Identification of shared and unique immunoglobulin E epitopes of the highly conserved tropomyosins in Blomia tropicalis and Dermatophagoides pteronyssinus. Clin Exp Allergy 32: 12031210.
  30. Reese G, Ayuso R, Lehrer SB, , 1999. Tropomyosin: an invertebrate pan-allergen? Int Arch Allergy Immunol 119: 247258.
  31. Lehrer SB, Ayuso R, Reese G, , 2003. Seafood allergy and allergens: a review. Mar Biotechnol (NY) 5: 339348.
  32. Reese G, Schicktanz S, Lauer I, Randow S, Lu¨ttkopf D, Vogel L, , 2006. Structural, immunological and functional properties of natural recombinant Pen a 1,the major allergen of brown shrimp, Penaeus aztecus. Clin Exp Allergy 36: 517524.
  33. Zhang R, ., 2012. Characterization and evaluation of a Sarcoptes scabiei allergen as a candidate vaccine. Parasit Vectors 5: 176184.
  34. Thomas WR, Smith W, , 1998. House-dust-mite allergens. Allergy 53: 821832.
  35. Tsai LC, Chao PL, Hung MW, Sun YC, Kuo IC, Chua KY, Liaw SH, , 2000. Protein sequence analysis and mapping of IgE and IgG epitopes of an allergenic 98-kDa Dermatophagoides farina paramyosin, Der f 11. Allergy 55: 141147.
  36. Thomas WR, , 2010. Geography of house dust mite allergens. Asian Pac J Allergy Immunol 28: 211224.
  37. Kenyon F, Welsh M, Parkinson J, Whitton C, Blaxter ML, Knox DP, , 2003. Expressed sequence tag survey of gene expression in the scab mite Psoroptes ovis allergens, proteases and free-radical scavengers. Parasitol 126: 451460.
  38. Huntley JF, Machell J, Nisbet AJ, Broek AD, Chua KY, Cheong N, Hales BJ, Thomas WR, , 2004. Identification of tropomyosin, paramyosin and apolipophorin/vitellogenin as three major allergens of the sheep scab mite, Psoroptes ovis. Parasite Immunol 26: 335342.
  39. Geoffrey NG, McManus DP, , 2005. Update on paramyosin in parasitic worms. Parasitol Int 54: 101107.
  40. Erban T, , 2011. Purification of tropomyosin, paramyosin, actin, tubulin, troponin and kinases for chemiproteomics and its application to different scientific fields. PLoS One 6: 19.
  41. Dahmen A, Gallin M, Schumacher M, Erttmann KD, , 1993. Molecular cloning and pre-mRNA maturation of Onchocerca volvulus paramyosin. Mol Biochem Parasitol 57: 335338.
  42. Song J, Li L, Zhigang L, Qiyuan L, Pixin R, , 2009. Sequence analysis and expression of a cDNA clone encoding tropomysin in Sinonovacula constricta. Mol Biol Rep 36: 315321.
  43. Mattson JG, Ljunggren EL, Bergstrom K, , 2001. Paramyosin from parasitic mite Sarcoptes scabiei: cDNA cloning and heterologous expression. Parasitol 122: 555562.
  44. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S, , 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30: 27252729.
  45. Nisbet AJ, Mackellar A, Wright HW, Brennan GP, Chua KY, Cheong N, Thomas JE, Huntley JF, , 2006. Molecular characterization, expression and localization of tropomyosin and paramyosin immuno-dominant allergens from sheep scab mites (Psoroptes ovis). Parasitol 133: 515523.
  46. Arlian LG, Vyszenski-Moher DL, Ahmed SG, Estes SA, , 1991. Cross-antigenicity between the scabies mite, Sarcoptes scabiei, and the house dust mite, Dermatophagoides pteronyssinus. J Invest Dermatol 96: 349361.
  47. Jeong KY, Yum HY, Lee IY, Ree HI, Hong CS, Kim DS, Yong TS, , 2004. Molecular cloning and characterization of tropomyosin, a major allergen of Chironomus kiiensis, a dominant species of non-biting midges in Korea. Clin Diagn Lab Immunol 11: 320324.
  48. Sookrung N, Chaicumpa W, Tungtrongchitr A, Vichyanond P, Bunnag C, , 2006. Periplaneta americana arginine kinase as a major cockroach allergen among Thai patients with major cockroach allergies. Environ Health Perspect 114: 875880.
  49. Seiki K, Oda H, Yoshioka H, Sakai H, Urisu A, Akiyama H, Ohno Y, , 2007. A reliable and sensitive immunoassay for the determination of crustacean protein in processed foods. J Agric Food Chem 55: 93459350.
  50. Santos ABR, Gutemberg MR, Constance O, Virginia PL, Rodrigo CL, Mario SP, Valeria SF, Rob CA, Martin DC, Arruda LK, , 2008. Cross-reactive IgE antibody responses to tropomyosins from Ascaris lumbricoides and cockroach. J Allergy Clin Immunol 121: 10401046.
  51. Walton SF, Holt DC, Currie BJ, Kemp DJ, , 2004. Scabies: a new future for neglected scabies. Adv Parasitol 57: 309376.
  52. Walton SF, Slender A, Pizzutto S, Mounsey K, Oprescu F, Thomas W, Hales B, Currie B, , 2015. Analysis of IgE binding patterns to house dust mite allergens in scabies endemic communities: insights for both diseases. Clin Exp Allergy 45: 18681872.
  53. Tsai LC, Chao PL, Shen HD, Tang RB, Chang TC, Chang ZN, Hung MW, Lee BL, Chua KY, , 1998. Isolation and characterization of a novel 98-kd Dermatophagoides farinae mite allergen. J Allergy Clin Immunol 102: 295303.
  54. Jiz M, ., 2009. Immunoglobulin E (IgE) responses to paramyosin predicts resistance to reinfestation with Schistosoma japonicum and are attenuated by IgG4. Infect Immun 77: 20512058.
  55. Gu XB, Yang GY, , 2008. A study on the genetic relationship of mites in the genus Sarcoptes (Acari: Sarcoptidae) in China. Int J Acarol 32: 183190.
  56. Gu X, Xie Y, Wang S, Peng X, Lai S, Yang G, , 2014. Immune response induced by candidate Sarcoptes scabiei var. cuniculi DNA vaccine encoding paramyosin in mice. Exp Appl Acarol 63: 401412.
  57. Beckham S, ., 2009. Characterization of a serine protease homologous to house dust mite group 3 allergens from the scabies mite Sarcoptes scabiei. J Biol Chem 284: 3441334422.
  58. Arlian LG, , 1988. Host specificity of S. scabiei var. canis (Acari: Sarcoptidae) and the role of host odor. J Med Entomol 25: 5256.
  59. Arlian LG, Morgan MS, , 2000. Serum antibody to Sarcoptes scabiei and house dust mite prior to and during infestation with S. scabiei. Vet Parasitol 90: 315326.
  60. Loukas A, Jones MK, King LT, Brindley PJ, McMannus DP, , 2001. Receptor for Fc on the surfaces of schistosomes. Infect Immun 69: 36463651.
  61. Ferreira CA, Barbosa MC, Silveira TC, Valenzuela JG, Da Silva Vaz Jr I, Masuda A, , 2002. cDNA cloning, expression and characterization of a Boophilus microplus paramyosin. Parasitol 125: 265274.
  62. Casais R, Granda V, Balseiro A, Cerro A, Dalton KP, González R, Bravo P, Prieto JM, Maria M, , 2016. Vaccination of rabbits with immunodominant antigens from Sarcoptes scabiei induced high levels of humoral responses and pro-inflammatory cytokines but confers limited protection. Parasit Vectors 9: 435.
  63. Rosner B, Spiegelman D, Willett WC, , 1990. Correction of logistic regression relative risk estimates and confidence intervals for measurement error: the case of multiple covariates measured with error. Am J Epidemiol 132: 734745.
  64. Arlian L, Morgan M, Estes S, Walton S, Kemp D, Currie BJ, , 2004. Circulating IgE in patients with ordinary and crusted scabies. J Med Entomol 41: 7477.
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  • Received : 12 Dec 2016
  • Accepted : 05 May 2017

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