Volume 97, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



We report the results of an investigation into a fatal case of hantavirus pulmonary syndrome (HPS) in Rio de Janeiro State, Brazil, where the disease had not been reported previous to 2015. Following the notification of an HPS case, serum samples were collected from the household members and work contacts of the HPS patient and tested for antibody to hantaviruses. Seroprevalence of 22% (10/45) was indicated for hantavirus out of 45 human samples tested. Blood and tissue samples were collected from 72 rodents during fieldwork to evaluate the prevalence of hantavirus infection, by using enzyme-linked immunosorbent assay IgG, and to characterize the rodent hantavirus reservoir(s), by reverse transcription polymerase chain reaction and sequencing. Antibody prevalence was 6.9%. The circulation of a single genotype, the Juquitiba hantavirus, carried by two rodent species, black-footed pigmy rice rat () and cursor grass mouse (), was shown by analysis of the nucleotide sequences of the S segment. Juquitiba hantavirus circulates in rodents of various species, but mainly in the black-footed pigmy rice rat. HPS is a newly recognized clinical entity in Rio de Janeiro State and should be considered in patients with febrile illness and acute respiratory distress.


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  1. Pan American Health Organization World Health Organization, 2013. Epidemiological Alert: Hantavirus Pulmonary Syndrome (HPS). October 17, 2013. Available at: http://www.paho.org/hq/index.php?option=com_docman&task=doc_view&gid=23388&Itemid=270. Accessed September 15, 2016. [Google Scholar]
  2. Figueiredo LT, Souza WM, Ferrés M, Enria DA, , 2014. Hantaviruses and cardiopulmonary syndrome in South America. Virus Res 187: 4354.[Crossref] [Google Scholar]
  3. World Health Organization (WHO), 2013. Sustaining the Drive to Overcome the Global Impact of Neglected Tropical Diseases: Second WHO Report on Neglected Diseases. Geneva, Switzerland: WHO. [Google Scholar]
  4. Akiner MM, Demirci B, Babuadze G, Robert V, Schaffner F, , 2016. Spread of the invasive mosquitoes Aedes aegypti and Aedes albopictus in the black sea region increases risk of chikungunya, dengue, and zika outbreaks in Europe. PLoS Negl Trop Dis 10: 15. [Google Scholar]
  5. Secretaria de Vigilância em Saúde/Ministério da Saúde (SVS/MS), 2016. Monitoramento dos Casos de Dengue, febre de Chikungunya e Febre Pelo Vírus Zika até a Semana Epidemiológica 37 de 2016. Boletim Epidemiológico Volume 47 - n° 34. Brasília: SVS/MS.
  6. Secretaria de Vigilância em Saúde/Ministério da Saúde (SVS/MS), 2014. Monitoramento dos Casos de Dengue Semana Epidemiológica (SE) 35 e Febre de Chikungunya SE 36 de 2014. Boletim Epidemiológico. Volume 45 - n° 20. Brasília: SVS/MS.
  7. Oliveira RC, Guterres A, Fernandes J, D'Andrea PS, Bonvicino CR, de Lemos ER, , 2014. Hantavirus reservoirs: current status with an emphasis on data from Brazil. Viruses 29: 19291973.[Crossref] [Google Scholar]
  8. Brazilian Ministry of Health, 2016. Confirmed Cases of Hantavirus Diseases. Brazil, Major Regions and Federative Units, 1993 to 2016 . Available at: http://portalsaude.saude.gov.br/index.php/o-ministerio/principal/secretarias/svs/hantavirose. Accessed July 20, 2016.
  9. Oliveira RC, Cordeiro-Santos M, Guterres A, Fernandes J, de Melo AX, João GA, Novais MA, Travassos da Rosa ES, Vasconcelos PF, Vilges de Oliveira S, de Albuquerque BC, de Lemos ER, , 2014. Rio Mamoré virus and hantavirus pulmonary syndrome, Brazil. Emerg Infect Dis 20: 15681570.[Crossref] [Google Scholar]
  10. Raboni SM, Levis S, Rosa ES, Bisordi I, Delfraro A, Lemos E, Correia DC, Duarte dos Santos CN, , 2007. Hantavirus infection in Brazil: development and evaluation of an enzyme immunoassay and immunoblotting based on N recombinant protein. Diagn Microbiol Infect Dis 58: 8997.[Crossref] [Google Scholar]
  11. Bonvicino CR, Otazú IB, Vilela JF, , 2005. Karyologic and molecular analysis of Proechimys Allen, 1899 (Rodentia, Echimyidae) from the Amazonian region. Arq Mus Nac (Rio de Janeiro) 63: 191200. [Google Scholar]
  12. Bonvicino CR, , 2011. Diversidade cariotipica em roedores Akodontini do Brasil. Bol Soc Bras Mastozoologia 62: 711. [Google Scholar]
  13. Padula PJ, Rossi CM, Della Valle MO, Martínez PV, Colavecchia SB, Edelstein A, Miguel SD, Rabinovich RD, Segura EL, , 2000. Development and evaluation of solid-phase enzyme immunoassay based on Andes hantavirus recombinant nucleoprotein. J Med Microbiol 49: 149155.[Crossref] [Google Scholar]
  14. Guterres A, de Oliveira RC, Fernandes J, Schrago CG, de Lemos ER, , 2015. Detection of different South American hantaviruses. Virus Res 210: 106113.[Crossref] [Google Scholar]
  15. Chu YK, Goodin D, Owen RD, Koch D, Jonsson CB, , 2009. Sympatry of 2 hantavirus strains, paraguay, 2003–2007. Emerg Infect Dis 15: 19771980.[Crossref] [Google Scholar]
  16. Oliveira RC, Teixeira BR, Mello FC, Pereira AP, Duarte AS, Bonaldo MC, Bonvicino CR, D'Andrea PS, Lemos ER, , 2009. Genetic characterization of a Juquitiba-like viral lineage in Oligoryzomys nigripes in Rio de Janeiro, Brazil. Acta Trop 112: 212218.[Crossref] [Google Scholar]
  17. Oliveira RC, Padula PJ, Gomes R, Martinez VP, Bellomo C, Bonvicino CR, Freire e Lima DI, Bragagnolo C, Caldas AC, D'Andrea PS, de Lemos ER, , 2011. Genetic characterization of hantaviruses associated with sigmodontine rodents in an endemic area for hantavirus pulmonary syndrome in southern Brazil. Vector Borne Zoonotic Dis 11: 301314.[Crossref] [Google Scholar]
  18. Sobreira M, Souza GT, Moreli ML, Borges AA, Morais FA, Figueiredo LT, Almeida AM, , 2008. A serosurvey for hantavirus infection in wild rodents from the states of Rio de Janeiro and Pernambuco, Brazil. Acta Trop 107: 150152.[Crossref] [Google Scholar]
  19. Raboni SM, Hoffmann FG, Oliveira RC, Teixeira BR, Bonvicino CR, Stella V, Carstensen S, Bordignon J, D'Andrea PS, Lemos ER, Duarte dos Santos CN, , 2009. Phylogenetic characterization of hantaviruses from wild rodents and hantavirus pulmonary syndrome cases in the state of Parana (southern Brazil). J Gen Virol 90: 21662171.[Crossref] [Google Scholar]
  20. Oliveira RC, Gentile R, Guterres A, Fernandes J, Teixeira BR, Vaz V, Valdez FP, Vicente LH, da Costa-Neto SF, Bonvicino C, D'Andrea PS, Lemos ER, , 2014. Ecological study of hantavirus infection in wild rodents in an endemic area in Brazil. Acta Trop 131: 110.[Crossref] [Google Scholar]
  21. Teixeira BR, Loureiro N, Strecht L, Gentile R, Oliveira RC, Guterres A, Fernandes J, Mattos LH, Raboni SM, Rubio G, Bonvicino CR, dos Santos CN, Lemos ER, D'Andrea OS, , 2014. Population ecology of hantavirus rodent hosts in southern Brazil. Am J Trop Med Hyg 91: 249257.[Crossref] [Google Scholar]
  22. Guterres A, de Oliveira RC, Fernandes J, Strecht L, Casado F, Gomes de Oliveira FC, D'Andrea PS, Bonvicino CR, Schrago CG, de Lemos ER Sampaio, , 2014. Characterization of Juquitiba virus in Oligoryzomys fornesi from Brazilian Cerrado. Viruses 6: 14731482.[Crossref] [Google Scholar]
  23. Umetsu F, Pardini R, , 2007. Small mammals in a mosaic of forest remnants and anthropogenic habitats—evaluating matrix quality in an Atlantic forest landscape. Landsc Ecol 22: 517530.[Crossref] [Google Scholar]
  24. Guterres A, de Oliveira RC, Fernandes J, D'Andrea PS, Bonvicino CR, Bragagnolo C, Guimarães GD, Almada GL, Machado RR, Lavocat M, Elkhoury Mda R, Schrago CG, de Lemos ER, , 2013. Phylogenetic analysis of the S segment from Juquitiba hantavirus: identification of two distinct lineages in Oligoryzomys nigripes . Infect Genet Evol 18: 262268.[Crossref] [Google Scholar]

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  • Received : 27 Oct 2016
  • Accepted : 26 Dec 2016
  • Published online : 17 Apr 2017

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