Volume 97, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Chagas disease is caused by and transmitted by feces of a triatomine that has the habit of defecating during blood feeding. The salivary glands of triatomines are important to hematophagy because their saliva is rich in anticoagulant and hemolytic proteins. The salivary glands of some species analyzed are reddish due to the presence of nitrophorins (antihemostatic activity). The present study aimed to analyze the color pattern of the salivary glands of 67 triatomine species to evaluate whether the presence of nitrophorins is a synapomorphy of or the tribe Rhodniini, or if it is shared with triatomines of the tribes Triatomini and Cavernicolini. Since only the species of the tribe Rhoniini present red glands, it is admitted that the presence of nitrophorin proteins is a synapomorphy of the tribe Rhodniini and that this tribe has derived more recently when compared with Triatomini and Cavernicolini.


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  1. World Health Organization, 2015. Chagas disease (American trypanosomiasis). Wkly Epidemiol Rec 90: 3344.
  2. Galvão C, , 2014. Vetores da Doença de Chagas no Brasil. Curitiba, Brazil: Sociedade Brasileira de Zoologia.
  3. Justi S, Galvão C, , 2017. The evolutionary origin of diversity in Chagas disease vectors. Trends Parasitol 33: 4252.
  4. Schofield CJ, Service MW, , 1988. The biosystematics of Triatominae. , ed. Biosystematics of Haematophagous Insects, Systematics Association Special. Oxford, United Kingdom: Clarenden Press, 284312.
  5. Hellmann K, Hawkins RI, , 1965. Prolixins-S and prolixin-G; two anticoagulants from Rhodnius prolixus Stal. Nature 207: 265267.
  6. Ribeiro JM, Hazzard JM, Nussenzveig RH, Champagne DE, Walker FA, , 1993. Reversible binding of nitric oxide by a salivary heme protein from a bloodsucking insect. Science 260: 539541.
  7. Lacombe D, , 1999. Anatomia e histologia de glândulas salivares de triatomíneos. Mem Inst Oswaldo Cruz 94: 557564.
  8. Montandon CE, Barros E, Vidigal PM, Mendes MT, Anhê ANBM, Ramos HJO, Oliveira CJF, Mafra C, , 2016. Comparative proteomic analysis of the saliva of the Rhodnius prolixus, Triatoma lecticularia and Panstrongylus herreri triatomines reveals a high interspecific functional biodiversity. Insect Biochem Mol Biol 71: 8390.
  9. Anhê AC, Azeredo-Oliveira MT, , 2008. Cytochemical characterization of Triatoma infestans and Panstrongylus megistus salivary gland cells (Hemiptera, Reduviidae, Triatominae). Micron 39: 11261133.
  10. Champagne DE, Nussenzveig RH, Ribeiro JMC, , 1995. Purification, partial characterization and cloning of nitric oxide-carrying heme proteins (nitrophorins) from salivary glands of the blood-sucking insect Rhodnius prolixus . J Biol Chem 270: 86918695.
  11. Montfort WR, Weichsel A, Andersen JF, , 2000. Nitrophorins and related antihemostatic lipocalins from Rhodnius prolixus and other blood-sucking arthropods. Biochim Biophys Acta 1482: 110118.
  12. Soares RPP, Gontijo NF, Romanha AJ, Diotaiuti L, Pereira MH, , 1998. Salivary heme proteins distinguish Rhodnius prolixus from Rhodnius robustus (Hemiptera: Reduviidae: Triatominae). Acta Trop 71: 285291.
  13. Pacheco DE, , 2014. Nitroforinas salivares de Rhodnius prolixus (Hemiptera: Reduviidae): Avaliação do RNAi parental e do papel da albumina do hospedeiro na sua atividade biológica. Available at: http://www.bibliotecadigital.ufmg.br/dspace/bitstream/handle/1843/BUOS-9KXJS7/dissertfinal.pdf?sequence=1. Accessed February 22, 2017.
  14. Meirelles RM, Rodrigues IS, Steindel M, Soares MJ, , 2003. Ultrastructure of the salivary glands of Rhodnius domesticus Neiva and Pinto, 1923 (Hemiptera: Reduviidae). J Submicrosc Cytol Pathol 35: 199207.
  15. Bargues MD, Marcilla A, Ramsey J, Dujardin JP, Schofield CJ, Mas-Coma S, , 2000. Nuclear rDNA-based molecular clock of the evolution of Triatominae (Hemiptera: Reduviidae), vectors of Chagas disease. Mem Inst Oswaldo Cruz 95: 567573.
  16. Justi SA, Galvão C, Schrago CG, , 2016. Geological changes of the Americas and their influence on the diversification of the neotropical kissing bugs (Hemiptera: Reduviidae: Triatominae). PLoS Negl Trop Dis 10: e0004527.
  17. Ribeiro JMC, Schneider M, Guimaraes JA, , 1995. Purification and characterization of Prolixin S (Nitrophorin 2), the salivary anticoagulant of the blood sucking bug, Rhodnius prolixus . Biochem J 308: 243249.
  18. Noeske-Jungblut C, Kratzschmar J, Haendler B, Alagon A, Possani L, Verhallen P, Donner P, Schleuning WD, , 1994. An inhibitor of collagen-induced platelet aggregation from the saliva of Triatoma pallidipennis . J Biol Chem 269: 50505053.
  19. Isawa H, Orito Y, Jingushi N, Iwanaga S, Morita A, Chinzei Y, Yuda M, , 2007. Identification and characterization of plasma kallikrein-kinin system inhibitors from salivar glands of the blood-sucking insect Triatoma infestans . FEBS J 274: 42714286.
  20. Ribeiro JMC, Assumpcao TC, Francischetti IMB, , 2012. An insight into the sialomes of bloodsucking Heteroptera. Psyche (Stuttg) 2012: 16.

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  • Received : 17 Oct 2016
  • Accepted : 03 Mar 2017

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