Volume 97, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Visceral leishmaniasis (VL) is a chronic parasitic disease associated with suppressed T cell responses. Although parasites reside intracellularly in macrophages during chronic VL, neutrophils are the first host cell to infiltrate the infection site and phagocytose the parasite. Subsets of neutrophils with unusual characteristics have been documented in human VL, but whether the total neutrophil population is aberrant during disease is not known. Therefore, we examined phenotypic characteristics of unfractionated polymorphonuclear leukocyte (neutrophils) from subjects with active VL, and compared these with neutrophils from healthy controls or subjects who have been treated for VL. The data showed decreased mRNA and diminished amounts of the neutrophil chemoattractant CXCL8 (interleukin [IL]-8), increased IL-10 mRNA and protein, and elevated transcripts encoding arginase-1, which is involved in suppressing T cell responses. Neutrophils from VL subjects showed enhanced capacity to phagocytose spp. promastigotes. The results suggest that neutrophils may contribute to immunosuppression in subjects with active VL.


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  1. World Health Organization, 2016. Leishmaniasis. Available at: http://www.who.int/topics/leishmaniasis/en/.
  2. Alvar J, Velez ID, Bern C, Herrero M, Desjeux P, Cano J, Jannin J, , den Boer M, 2012. Leishmaniasis worldwide and global estimates of its incidence. PLoS One 7: e35671. [Google Scholar]
  3. Peters NC, Sacks DL, , 2009. The impact of vector-mediated neutrophil recruitment on cutaneous leishmaniasis. Cell Microbiol 11: 12901296. [Google Scholar]
  4. Charmoy M, Auderset F, Allenbach C, Tacchini-Cottier F, , 2010. The prominent role of neutrophils during the initial phase of infection by Leishmania parasites. J Biomed Biotechnol 2010: 719361. [Google Scholar]
  5. Mocsai A, , 2013. Diverse novel functions of neutrophils in immunity, inflammation, and beyond. J Exp Med 210: 12831299. [Google Scholar]
  6. Jaillon S, Galdiero MR, Del Prete D, Cassatella MA, Garlanda C, Mantovani A, , 2013. Neutrophils in innate and adaptive immunity. Semin Immunopathol 35: 377394. [Google Scholar]
  7. Abebe T, , et al., 2013. Arginase activity: a marker of disease status in patients with visceral leishmaniasis in ethiopia. PLoS Negl Trop Dis 7: e2134. [Google Scholar]
  8. Sharma S, Davis RE, Srivastva S, Nylen S, Sundar S, Wilson ME, , 2016. A subset of neutrophils expressing markers of antigen-presenting cells in human visceral leishmaniasis. J Infect Dis 214: 15311538. [Google Scholar]
  9. Gupta G, Oghumu S, Satoskar AR, , 2013. Mechanisms of immune evasion in leishmaniasis. Adv Appl Microbiol 82: 155184. [Google Scholar]
  10. Boelaert M, , et al., 2008. Diagnostic tests for kala-azar: a multi-centre study of the freeze-dried DAT, rK39 strip test and KAtex in East Africa and the Indian subcontinent. Trans R Soc Trop Med Hyg 102: 3240. [Google Scholar]
  11. Jeronimo SMB, , Sousa AdQ, Pearson RD, 2006. Leishmaniasis. Guerrant RL, Walker DH, Weller PF, eds. Tropical Infectious Diseases. Philadelphia, PA: Elsiever, Inc., 1095–1113.
  12. Chang HK, Thalhofer C, Duerkop BA, Mehling JS, Verma S, Gollob KJ, Almeida R, Wilson ME, , 2007. Short term fluorescent labeling of a protozoan parasite to assess phagocytosis and oxidant generation. Infect Immun 75: 10171024. [Google Scholar]
  13. Metcalf JA, Gallin JI, Nauseef WM, Root RK, , 1986. Laboratory Manual of Neutrophil Function. New York, NY: Raven Press.
  14. Davis RE, Sharma S, Conceicao J, Carneiro P, Novais F, Scott P, Sundar S, Bacellar O, Carvalho EM, Wilson ME, , 2017. Phenotypic and functional characteristics of HLA-DR+ neutrophils in Brazilians with cutaneous leishmaniasis. J Leukoc Biol 101: 739749. [Google Scholar]
  15. Ueno N, Bratt CL, Rodriguez NE, Wilson ME, , 2009. Differences in human macrophage receptor usage, lysosomal fusion kinetics and survival between logarithmic and metacyclic Leishmania infantum chagasi promastigotes. Cell Microbiol 11: 18271841. [Google Scholar]
  16. Ueno N, Wilson ME, , 2012. Receptor-Mediated Phagocytosis of Leishmania: implications for intracellular survival. Trends Parasitol 28: 335344. [Google Scholar]
  17. Kumar R, Nylen S, , 2012. Immunobiology of visceral leishmaniasis. Front Immunol 3: 251. [Google Scholar]
  18. Kim GY, Lee JW, Ryu HC, Wei JD, Seong CM, Kim JH, , 2010. Proinflammatory cytokine IL-1beta stimulates IL-8 synthesis in mast cells via a leukotriene B4 receptor 2-linked pathway, contributing to angiogenesis. J Immunol 184: 39463954. [Google Scholar]
  19. Munder M, , et al., 2006. Suppression of T-cell functions by human granulocyte arginase. Blood 108: 16271634. [Google Scholar]
  20. Carvalho EM, Teixeira RS, Johnson JWD, , 1981. Cell-mediated immunity in American visceral leishmaniasis: reversible immunosuppression during acute infection. Infect Immun 33: 498502. [Google Scholar]
  21. Nauseef WM, , 2014. Detection of superoxide anion and hydrogen peroxide production by cellular NADPH oxidases. Biochim Biophys Acta 1840: 757767. [Google Scholar]

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  • Received : 05 Sep 2016
  • Accepted : 01 Jun 2017
  • Published online : 31 Jul 2017

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